Objective:To explore the effect of RNA binding ZCCHC17 on proliferation,migration and invasion ability of hepato-cellular carcinoma(HCC).Methods:RT-qPCR and Western blot were used to detect the expression levels of the cell lines ZCCHC17 selected.Then,the proliferation,migration,and invasion ability of cell lines were examined by clonformation assay,CCK-8 assay,scratch assay and Transwell invasion assay.Results:ZCCHC 17 was lowly expressed in human normal liver cell line L02(P＞0.05),high ex-pression was observed in the Bel7404 hepatocellular carcinoma cell line(P＜0.05),and low expression was observed in the MH-CC97H hepatocellular carcinoma cell line was diminished after ZCCHC17 knockdown(P＞0.05).The results of clone formation assay and CCK-8 showed that the proliferation of Bel7404 cell lines was diminished after ZCCHC17 knockdown(P＜0.05),the scratch test results showed that the migration ability of Bel7404 cell lines decreased(P＜0.05),the Transwell experiment showed that the invasion ability of Bel7404 cell lines was weakened(P＜0.05).Conclusion:High expression of ZCCHC17 can promote the proliferation,migra-tion and invasion of hepatocellular carcinoma.