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The mechanism of vitamin D regulating autophagy activity and FXR/FGF15 signaling pathway improving type 2 diabetes in rats
Zhao Yueping; Liu Ming; Zhang Qi; Zhang Rui
2026,41(2):129-135 ,DOI: 10.3969/j.issn.1005-3697.2026.02.001
Abstract:
Objective: To explore the mechanism of action of vitamin D in regulating autophagy activity and improving type 2 diabetes in rats through the FXR/FGF15 signaling pathway.Methods: 40 SPF-grade SD rats were selected. Among them, 10 rats were set as the control group, and 30 were used to establish the type 2 diabetes model. The 27 successfully modeled rats were divided into a model group, a vitamin D group, and a vitamin D + FXR inhibitor group, with 9 rats in each group. The histopathological changes in renal tissue, fasting blood glucose, insulin, body weight, renal function, inflammatory factor levels, autophagy-related proteins, and mRNA and protein expression levels of the FXR/FGF15 pathway in rats from each group were compared.Results: The renal tissue structure of rats in the control group was normal. In the model group, the rats exhibited glomerular hypertrophy, mesangial cell proliferation, and swelling, degeneration, and partial atrophy of renal tubular epithelial cells. The renal pathological damage in the vitamin D group was alleviated, while the damage in the vitamin D + FXR inhibitor group was more severe than that in the vitamin D group, with a degree between that of the model group and the vitamin D group. Compared with the control group, the model group rats exhibited elevated fasting blood glucose, creatinine (Scr), blood urea nitrogen (BUN), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and P62 protein expression. The mRNA and protein expression levels of insulin, body mass, lysosomal-associated membrane protein 1 (LAMP1), LC3-II/I protein, farnesoid X receptor (FXR), and fibroblast growth factor 15 (FGF15) decreased, with statistically significant differences (P < 0.05). Compared with the model group, the vitamin D group exhibited reduced fasting blood glucose, Scr, BUN, MCP-1, IL-6, IL-1β, TNF-α, and P62 protein expression, while insulin, body weight, LAMP1, LC3-II/I protein, FXR, FGF15 mRNA, and protein expression levels were increased (P < 0.05). Compared with the vitamin D group, the rats in the vitamin D + FXR inhibitor group exhibited increased fasting blood glucose, Scr, BUN, MCP-1, IL-6, IL-1β, TNF-α, and P62 protein expression, and decreased insulin, body weight, LAMP1, LC3-II/I protein, FXR, FGF15 mRNA, and protein expression levels, with statistically significant differences (P < 0.05). Conclusion: Vitamin D can improve renal function, and its mechanism of action may be related to regulating autophagy activity and the FXR/FGF15 pathway.
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Preliminary study on the induction of early dry age-related macular degeneration in mice using low-dose sodium iodate
Wang Longlong; Liu Xinqu; Meng Lei; Han Ziman
2026,41(2):136-140 ,DOI: 10.3969/j.issn.1005-3697.2026.02.002
Abstract:
Objective: To investigate the feasibility of establishing an early-stage age-related macular degeneration (AMD) model in C57BL/6J mice using low-dose sodium iodate (NaIO?).Methods: 24 nine-week-old specific pathogen-free (SPF) male C57BL/6J mice were randomly assigned to four groups (n=6/group): control, 10 mg/kg NaIO?, 15 mg/kg NaIO?, and 20 mg/kg NaIO?. Injury groups received a single intraperitoneal injection of NaIO?, while controls received sterile phosphate-buffered saline (PBS). At 1, 2, and 4 weeks post-injection, two mice per group were euthanized for bilateral eyeball enucleation. Retinal morphology was assessed using hematoxylin-eosin (H&E) staining of paraffin-embedded sections.Results: With the increase of sodium iodate concentration and the prolongation of treatment time, the structure of mouse retinal tissue gradually loosened, the nerve fiber layer (NFL) gradually shrank and became thinner (P < 0.05), and ganglion cell layer (GCL) cells and retinal pigment epithelium (RPE) cells gradually decreased (P < 0.05). As the concentration of sodium iodate gradually increased to 20 mg/kg and the treatment time was extended to 2 weeks, irregular deposits were observed beneath the mouse RPE layer.Conclusion: Low-dose NaIO? effectively induces early AMD-like pathology in mice, supporting its utility for mechanistic studies.
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Screening of key genes and signaling pathways in osteoarthritis based on bioinformatics
Aixin; Xie Yujie; Luo Xi; Zhang Chi
2026,41(2):141-148 ,DOI: 10.3969/j.issn.1005-3697.2026.02.003
Abstract:
Objective: To mine key genes and pathways of osteoarthritis (OA) based on bioinformatics and validate them using an in vitro chondrocyte model.Methods: This study was based on the GSE269735 dataset from the GEO database, using Weighted Gene Co-expression Network Analysis (WGCNA) to identify key gene modules, combined with differential expression gene (DEG) analysis to screen candidate genes. GO and KEGG pathway analyses were performed on the common genes between DEGs and WGCNA key gene modules, with the STRING database and Cytoscape software used to identify key genes among the DEGs. To validate the robustness of the screening results, the GSE2204879 dataset was used for cross-validation, and a plasmid was constructed with CSF-1 as the candidate gene for transfection interference experiments in OA chondrocytes. Finally, the expression changes of CSF-1 at the cellular level were detected by Western blot, and its effects on cellular function were evaluated to verify its potential role in OA.Results: WGCNA analysis identified multiple modules closely related to OA phenotypes, and key genes were co-screened with DEGs. GO and KEGG analysis showed that the main biological processes involved cytokine activity, chemokine activity, collagen binding, etc. Key genes included CCL20, CSF-1, CXCL11, CXCL3, CXCL5, and CXCR4. In the GSE2204879 dataset, CSF-1 and CXCR4 still showed a consistent expression trend. At the cellular level, CSF-1 and CXCR4 were upregulated in OA model cells. Furthermore, a CSF-1 knockdown plasmid was constructed and transfected into OA chondrocytes; the results showed that CSF-1 knockdown suppressed CXCR4 expression, reduced the expression levels of MMP3 and MMP13 in the cells, and increased the protein expression level of Col2a1, suggesting that CSF-1 may participate in the OA inflammatory response and matrix degradation process by regulating CXCR4. Conclusion: This study, combining bioinformatics and cellular experiments, has screened out multiple key genes closely related to OA. Among them, CSF-1 and CXCR4 show high expression in OA. The experiments indicate that CSF-1 may participate in the OA inflammatory response and matrix degradation process by regulating CXCR4 expression. In summary, CSF-1 may be a key pathogenic factor and potential therapeutic target for OA.
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Expression and significance of CD163 and CD105 in colorectal cancer tissues
Jing Min; Zhang Guoping; Dai Yanbo
2026,41(2):149-153 ,DOI: 10.3969/j.issn.1005-3697.2026.02.004
Abstract:
Objective: To observe the expression of CD163 and CD105 in colorectal cancer tissues and explore their clinical significance.Methods: 82 pathological specimens of colon cancer and corresponding normal tissues adjacent to the cancer were collected from our hospital following surgical resection. Immunohistochemistry (IHC) was used to detect the expression of CD163 and CD105 in the two tissue types, and the difference in positive high expression rates of CD163 and CD105 was compared. The differences between the different expression patterns of CD163 and CD105 and clinical parameters of patients, as well as the correlation between CD163 and CD105 expression in colorectal cancer tissues, were analyzed.Results: The positive high expression rates of CD163 and CD105 in colorectal cancer tissues were 68.3% and 53.7%, respectively, while those in normal tissues adjacent to the cancer were 51.2% and 0%, respectively, with statistical significance (P0.05). Immunohistochemical quantitative detection confirmed a positive correlation between the expression levels of CD163 and CD105 in colorectal cancer tissues (P<0.05).Conclusion: The high expression of CD163 and CD105 is associated with tumor TNM staging and lymph node metastasis, and their expression in colorectal cancer tissues is positively correlated. This suggests that the combined high expression pattern of CD163 and CD105 is related to the progression of colorectal cancer, and its related molecular mechanisms can be further studied.
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Effect of Niuhuang Qingxin Pill on serum inflammatory factors and neurotransmitters in elderly patients with hypertension and vertigo accompanied by anxiety and depression
2026,41(2):154-159 ,DOI: 10.3969/j.issn.1005-3697.2026.02.005
Abstract:
Objective: To explore the effects of Niuhuang Qingxin Pill on serum inflammatory factors and neurotransmitters in elderly patients with hypertension-induced vertigo accompanied by anxiety and depression.Methods: 122 elderly patients with hypertensive vertigo with anxiety and depression were divided into an observation group (n=61) and a control group (n=61). The control group was given conventional Western medicine treatment, while the observation group was given Niuhuang Qingxin Pill on top of that. All were treated for 7 days. The changes in TCM syndrome scores, Self-Rating Anxiety Scale (SAS) scores, Self-Rating Depression Scale (SDS) scores, ECG function [Standard deviation of all NN intervals (SDNN), root mean square of successive RR interval differences (rMSSD), percentage of all successive RR interval differences greater than 50ms (pNN50), very low frequency power (VLF)], blood pressure, inflammatory factors [interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α)], and neurotransmitters [5-hydroxytryptamine (5-HT), norepinephrine (NE), dopamine (DA)] levels were observed in both groups before and after treatment. The clinical efficacy and adverse reactions of the two groups of patients were compared.Results: After 7 days of treatment, the total effective rate of the observation group was 91.80%, while that of the control group was 75.41%; the clinical efficacy of the observation group was higher than that of the control group (P0.05). After 3 months of follow-up, there were no delayed adverse reactions in the two groups; the incidence of recurrent hypertension vertigo, anxiety progression, and depression progression in the observation group was lower than that in the control group (P<0.05).Conclusion: Niuhuang Qingxin Pills can effectively inhibit the level of inflammatory factors, up-regulate the level of neurotransmitters, control blood pressure, improve neurological function, relieve vertigo, anxiety, depression, and other clinical symptoms in the treatment of elderly patients with hypertensive vertigo with anxiety and depression. The curative effect is remarkable, it can maintain the medium and long-term curative effect, and the safety is reliable.
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Gastric filling ultrasound study of gastric dynamic function in obese patients with different BMI levels
Yang Hang; Liu Yanzhou; Zhang Xiyue; Yue Wensheng
2026,41(2):160-165 ,DOI: 10.3969/j.issn.1005-3697.2026.02.006
Abstract:
Objective: To investigate the differences in gastric motility function among populations with different body mass index (BMI) levels using gastric filling ultrasound, clarify the independent impact of BMI on gastric motility, and provide a basis for the research on the mechanism of obesity-related gastric motility disorders.Methods: A total of 100 subjects with BMI ≥ 25.0 kg/m2 were enrolled and divided into Group A (overweight group: 25.0 kg/m2 ≤ BMI 0.05). Within 3~5 minutes after drinking contrast agent, compared with the control group, Group A and Group B showed significant decreases in gastric peristaltic wave peak intensity (1.02±0.29 vs. 0.71±0.30 vs. 1.34±0.38) cm, gastric peristaltic velocity (0.28±0.08 vs. 0.24±0.07 vs. 0.31±0.07) cm/s, MI (0.35±0.11 vs. 0.28±0.10 vs. 0.45±0.23) cm2/s, and number of gastric peristalsis (6.15±1.28 vs. 5.61±1.32 vs. 6.72±1.29) times/2 minutes. Moreover, these indicators in Group B were lower than those in Group A, with statistically significant differences (P Group A > Group B”, with statistically significant differences (P0.05).Conclusion: After excluding confounding factors, BMI is negatively correlated with gastric motility function. The higher the BMI, the weaker the gastric peristaltic capacity and gastric emptying efficiency. Gastric filling contrast-enhanced ultrasound is a potential preferred examination method for evaluating gastric motility function in populations with different BMI levels due to its real-time, non-invasive, highly operable, and reproducible characteristics.
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Clinical practice of hierarchical antibody identification and limited-compatibility transfusion strategy in AIHA patient
Guo Xia; He Wei; Wang Yuanhua; Guo Yunfeng
2026,41(2):166-168+183 ,DOI: 10.3969/j.issn.1005-3697.2026.02.007
Abstract:
Objective: To investigate detection strategies for autoantibodies coexisting with alloantibodies and alloantibody-like specific antibodies in patients with autoimmune hemolytic anemia (AIHA), providing a theoretical basis for formulating safe and effective transfusion protocols in complex crossmatching scenarios.Methods: Serum autoantibodies and combined antibodies were detected using a combination of saline method, classic anti-human globulin test (Coombs test), and microcolumn gel assay. Antibody specificity and titer were determined through panel cell reaction pattern analysis, absorption/elution tests, and antibody titration.Results: Serum analysis revealed the presence of autoantibodies, anti-E alloantibody (titer: 1), anti-Wra alloantibody, and an antibody mimicking anti-Ce specificity (titer: 4). Crossmatch tests showed that the strong agglutination (2+) in the major crossmatch with group A, CCDee washed red blood cells (RBCs), weak agglutination (±) in the major crossmatch with group A, ccDEE washed RBCs. Consequently, 2 units of group A, ccDEE washed RBCs were transfused. The patient exhibited a satisfactory increase in hemoglobin post-transfusion, confirming transfusion efficacy without adverse reactions.Conclusion: In patients with multiple coexisting antibodies, priority must be given to excluding autoantibody interference before selecting the most compatible RBCs for transfusion based on antibody affinity and clinical urgency. The combined application of serological techniques and individualized transfusion strategies enhances transfusion safety in patients with complex serology.
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Clinical efficacy of concentrated growth factor filling combined with carbon dioxide fractional laser treatment for atrophic scars following subcutaneous dissection
Peng Cheng; Zhang Xinjie; Li Dailing; Ming Haixia; Li Xiaoping
2026,41(2):169-173 ,DOI: 10.3969/j.issn.1005-3697.2026.02.008
Abstract:
Objective: To explore the clinical efficacy of concentrated growth factor (CGF) filling combined with carbon dioxide (CO2) fractional laser treatment for atrophic scars following subcutaneous separation surgery.Methods: A total of 82 patients with depressed scars were enrolled as study subjects. They were grouped according to treatment modality into a control group (CO2 fractional laser therapy) and an observation group (CO2 fractional laser therapy combined with subcutaneous separation followed by CGF filling), with 41 cases in each group. The efficacy assessed by the Goodman & Baron Scar Grading Scale, Visual Analogue Scale (VAS), treatment satisfaction, Global Aesthetic Improvement Scale (GAIS), Self-Rating Anxiety Scale (SAS), duration of erythema and edema, healing time, incidence of hyperpigmentation, time off work, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), and adverse reactions were recorded and compared between the two groups.Results: At 2 months post-treatment, the observation group exhibited lower Goodman & Baron scar grade scores, pre- and post-treatment difference scores, IL-6, TNF-α, and INF-γ levels compared to the control group (P0.05).Conclusion: Subcutaneous separation followed by CGF filling combined with CO2 fractional laser treatment demonstrates significant efficacy in treating atrophic scars, reducing long-term inflammatory responses and anxiety levels while enhancing patient satisfaction with treatment outcomes.
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A prospective study on the construction of a predictive model for the efficacy of neoadjuvant chemotherapy in breast cancer by multimodal ultrasound evaluation of tumor biological behavior combined with systemic immune inflammatory index
Song Hongyu; Li Jingyan; Liu Baoman; Zhang Zhen
2026,41(2):174-178 ,DOI: 10.3969/j.issn.1005-3697.2026.02.009
Abstract:
Objective: To evaluate the predictive value of a predictive model for the efficacy of neoadjuvant chemotherapy (NAC) in breast cancer by multimodal ultrasound assessment of tumor biological behavior combined with systemic immune inflammation index.Methods: 98 patients with breast cancer were selected as prospective cohort study subjects. According to the NAC Miller-Payne grading system, it was divided into an effective group (MP4-5 grade, n=62) and ineffective group (MP1-3 level, n=36). All patients underwent multimodal ultrasound examination (including two-dimensional color Doppler, elastography, and contrast-enhanced ultrasound) before NAC to evaluate tumor blood flow (Adler grade), stiffness strain ratio (SR), perfusion peak intensity (PI) and time to peak (TTP). Using a fully automated blood cell analyzer to detect and calculate the Systemic Inflammatory Index (SII) based on the absolute value parameters in the blood routine report, and performing Miller-Payne grading of surgical pathology after NAC. Establish a joint prediction model using multiple logistic regression (Forward LR method), ROC curve analysis, calculate AUC, sensitivity, and specificity, Delong test compared the differences in curves.Results: The Adler blood flow grading, strain rate ratio, and peak intensity of the effective group were significantly lower than those of the ineffective group (P<0.001). On the contrary, the peak time of the effective group was significantly higher than that of the ineffective group (P<0.001). The SII, neutrophil count, and platelet count of the effective group were significantly lower than those of the ineffective group (P<0.05). On the contrary, the lymphocyte count in the effective group was significantly higher than that in the ineffective group (P<0.001). Multivariate Logistic regression analysis showed that tumor diameter, Adler blood flow grading, SR, PI, TTP, and SII were independent predictors of treatment efficacy (P<0.05). After multivariable adjustment, SR, PI, and SII remained independent predictors of treatment efficacy (P<0.05). ROC curve analysis showed that SR, PI, and SII all had good predictive value for efficacy failure, and the combined model significantly improved predictive performance.Conclusion: Multimodal ultrasound is used to evaluate the biological characteristics of tumor and SII to reflect the immune and inflammatory state of the body. The combination of the two can better indicate the development trend of the disease and the chemosensitivity of breast cancer patients when judging their condition.
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Application effect of double fixed laparoscopic jejunostomy in total endoscopic Ivor-Lewis operation for esophageal cancer
2026,41(2):179-183 ,DOI: 10.3969/j.issn.1005-3697.2026.02.010
Abstract:
Objective: To explore the application effect of double fixed laparoscopic jejunostomy in total endoscopic Ivor-Lewis operation for esophageal cancer. Methods: A retrospective analysis was conducted on 106 patients who underwent totally laparoscopic Ivor-Lewis esophagectomy performed by the same surgical team. Patients were divided into an observation group and a control group based on the jejunostomy technique used, with 53 cases in each group. The control group underwent traditional sutured laparoscopic jejunostomy, while the observation group underwent double-fixation laparoscopic jejunostomy. The postoperative jejunostomy tube removal time and time to first flatus were compared between the two groups. The inflammatory stress indicators [norepinephrine (NE), cortisol (COR), C-reactive protein (CRP)] before and 4 weeks after surgery were detected and compared. The levels of nutritional markers [serum albumin (ALB), globulin (GLB), hemoglobin (Hb)] were measured and compared before, 4 and 12 weeks after operation. The incidence of complications was also recorded for each group. Results: The observation group had shorter times to stoma tube removal and first anal exhaust compared to the control group (P0.05). Conclusion: Double-fixed laparoscopic jejunostomy combined with Ivor-Lewis operation can effectively reduce the incidence of postoperative intestinal obstruction, relieve inflammatory stress, and improve the nutritional status of patients, which is worth popularizing.
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Clinical efficacy of modified Buyang Huanwu Decoction combined with acupuncture in treating post-stroke Qi deficiency and blood stasis syndrome in patients with cerebral infarction
Chen Meng; Wang Yunfang; Yuan Luping; Sundy
2026,41(2):184-188 ,DOI: 10.3969/j.issn.1005-3697.2026.02.011
Abstract:
Objective: To explore the clinical outcomes of using modified Buyang Huanwu Decoction alongside acupuncture in treating post-stroke Qi deficiency and blood stasis syndrome in cerebral infarction patients. Methods: A retrospective analysis was conducted on 102 patients with Qi deficiency and blood stasis syndrome after cerebral infarction. The patients were divided into two groups based on treatment methods: the control group (receiving conventional treatment + acupuncture, n=51) and the observation group (receiving Buyang Huanwu Decoction with modifications in addition to the treatment in the control group, n=51). Both groups were treated for 4 weeks. The clinical efficacy, Traditional Chinese Medicine (TCM) syndrome scores before and after treatment, Barthel Index, muscle strength grading, Water Swallowing Test (WST) grading, coagulation function, and safety were compared between the two groups. Results: The observation group achieved a higher overall effectiveness rate than the control group (P0.05). Conclusion: Modified Buyang Huanwu Decoction combined with acupuncture can improve clinical symptoms, muscle strength, swallowing ability, daily living capacity, and coagulation function in patients with post-stroke Qi deficiency and blood stasis syndrome, without increasing the incidence of adverse reactions.
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Correlation analysis between spontaneous nystagmus and vestibular function in patients with acute vestibular neuritis
Wen Yu; Liu Cuiping; Zhong Cuiping
2026,41(2):189-192+205 ,DOI: 10.3969/j.issn.1005-3697.2026.02.012
Abstract:
Objective: To analyze the correlation between the intensity of spontaneous nystagmus (SN) and vestibular function in patients with acute vestibular neuritis (VN), and to clarify the quantitative relationship between the degree of semicircular canal dysfunction and the characteristics of SN. Methods: A total of 251 inpatients diagnosed with acute VN were enrolled in this study. All participants underwent comprehensive assessments including electronystagmography, rotational chair stop test, video head impulse test (v-HIT), and Dizziness Handicap Inventory (DHI) evaluation. Based on the intensity of SN, patients were stratified into three groups: 0~5°, 5~15°, and >15°. Statistical analyses were conducted to: compare DHI scores and rotational chair stop test Canal Paresis (CP) values among the three SN-intensity groups, evaluate the prevalence of abnormal v-HIT gain values in semicircular canals and compare horizontal, anterior, and posterior semicircular canal v-HIT gain values across the three SN-intensity subgroups. Results: A total of 251 acute VN patients were stratified into three groups by SN intensity: 0~5° (n=51, 20.32%), 5~15° (n=132, 52.59%), and >15° (n=68, 27.09%). Both DHI scores and CP values increased with SN intensity, and the differences between the three groups were statistically significant (P0.05). No significant between-group differences were observed in affected-to-healthy side v-HIT gain ratios across all three semicircular canals (P >0.05). Conclusion: In acute VN, SN intensity serves as a composite indicator of overall vestibular system involvement (encompassing peripheral lesion extent and central compensation status), likely reflecting widespread vestibular nerve involvement or abnormal central regulatory mechanisms rather than mere semicircular canal anatomical damage.
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Influence of ultrasound-guided axillary brachial plexus block anesthesia on block analgesia status in children with distal ulna and radius fractures in emergency room
Xie Guo; Zhang Jin; Du Yanshu; Yan Yalan
2026,41(2):193-196 ,DOI: 10.3969/j.issn.1005-3697.2026.02.013
Abstract:
Objective: To explore the influence of ultrasound-guided axillary brachial plexus block anesthesia on block analgesia status in children with distal ulna and radius fractures in emergency room. Methods: A total of 106 children with distal ulna and radius fractures were selected as research subjects. According to different reduction methods of fractures, the children were divided into control group (n=36, routine manipulation reduction) and painless treatment group (n=70, ultrasound-guided axillary brachial plexus block anesthesia manipulation reduction). The children in painless treatment group were further divided into group A (n=35, 1.0% lidocaine) and group B (n=35, 0.8% lidocaine) according to the different concentrations of lidocaine used. The block onset time, duration of analgesia and success rate of block were compared between group A and group B. The scores of Children's Hospital of Eastern Ontario Pain Scale (CHEOPS) before reduction (T0), during reduction (T1) and at 1 h after reduction (T2), the excellent and good rate of manipulation reduction, satisfaction of medical staff, satisfaction of parents and occurrence of adverse reactions were compared among the three groups. Results: The onset time of block in group A was shorter than that in group B (P0.05). At T1 and T2 the CHEOPS score in group A and group B was lower than that in control group (P 0.05). There was no statistically significant difference in the incidence of adverse reactions among group A, group B, and the control group (P>0.05). Conclusion: In emergency room environment, the application of ultrasound-guided brachial plexus block in children with distal radius and ulna fractures is an efficient and safe analgesic strategy, which can improve the quality of reduction and the satisfaction of both doctors and patients. 0.8% lidocaine is sufficient to meet the analgesic needs of manipulation reduction.
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Evaluated value of renal resistance index combined with serum CysC level in the evaluation of acute kidney injury by ultrasound
2026,41(2):197-200 ,DOI: 10.3969/j.issn.1005-3697.2026.02.014
Abstract:
Objective: To explore the evaluated value of ultrasound evaluation of renal resistance index (RRI) combined with serum cystatin C (CysC) level on acute kidney injury (AKI). Methods: The clinical data of 86 patients with AKI were retrospectively analyzed. According to the Kidney Disease Improving Global Outcomes (KDIGO) staging, they were divided into mild group (n=50) and severe group (n=36). The AKI staging was recorded, and the general data, RRI and CysC were compared between groups. ROC curve was drawn to evaluate the predictive value of CysC, RRI and their combination on patients with severe AKI. Spearman correlation analysis was used to analyze the correlation between RRI and CysC levels and AKI staging. Results: There were no statistical differences in gender and age between the two groups of patients (P>0.05). Statistically significant differences were observed in concurrent hypertension, concurrent diabetes mellitus and etiology in severe group compared with those in mild group (P<0.05). The RRI and CysC in severe group were higher than those in mild group, with statistical differences (P<0.05). ROC curve analysis showed that the AUCs of CysC, RRI and combined prediction in predicting severe AKI were 0.807, 0.773 and 0.903. The optimal cut-off value of CysC was 1.96 μmol/L, with a sensitivity of 68.00% and a specificity of 86.11%. The optimal cut-off value of RRI was 0.68, and the sensitivity and specificity were 58.00% and 86.11%. The sensitivity and specificity of combined prediction were 100.00% and 74.00%, respectively. Spearman correlation analysis showed that RRI and CysC were positively correlated with AKI staging (P<0.05). Conclusion: Most patients with AKI are in the early stage, and hypertension and diabetes mellitus are related to the disease severity. Ultrasound evaluation of RRI combined with serum CysC alone and in combination in predicting severe AKI has value. Combined prediction can enhance the predictive sensitivity, and the levels of the two are positively correlated with AKI staging.
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The therapeutic effect of supplementing vitamins A and D on preeclampsia patients with gestational hypertension
Chen Jinghua; Cheng Xi; Huang Qiaoru; Huang Suran
2026,41(2):201-205 ,DOI: 10.3969/j.issn.1005-3697.2026.02.015
Abstract:
Objective: To investigate the clinical efficacy of supplementing vitamins A and D in the treatment of preeclampsia (PE) patients with gestational hypertension. Methods: 86 patients with gestational hypertension and PE were enrolled and divided into a control group (n=43, 50.0%) and a study group (n=43, 50.0%) based on different treatment regimens. The control group received magnesium sulfate + nifedipine for blood pressure control and seizure prophylaxis, while the study group received additional vitamin A and D as adjunctive therapy alongside magnesium sulfate and nifedipine. Changes in blood pressure, catecholamine levels, and inflammatory/endothelial factors were compared between the two groups before and after treatment. Results: After treatment, both groups of PE patients showed a decrease in systolic blood pressure (SBP) and diastolic blood pressure (DBP) compared to before treatment (P<0.05). The post-treatment systolic blood pressure (142.52±9.41) mmHg and diastolic blood pressure (90.40±8.11) mmHg in the study group were significantly lower than those in the control group (158.01±10.32) mmHg and (98.31±9.22) mmHg, respectively (P<0.05). After treatment, dopamine, norepinephrine, and epinephrine levels decreased significantly in both groups of PE patients compared to before treatment (P<0.05), with the study group was lower than the control group (P <0.05). After treatment, tumor necrosis factor-α (TNF-α), endothelin-1 (ET-1), and C-reactive protein (CRP) levels decreased significantly in both groups of PE patients compared to before treatment (P<0.05), with the study group was lower than the control group (P<0.05). Vascular endothelial growth factor (VEGF) and placental growth factor (PLGF) levels increased significantly in both groups after treatment compared to before treatment (P<0.05), with the study group was higher than the control group (P<0.05). Conclusion: Supplementation with vitamins A and D, in addition to conventional anticonvulsant and antihypertensive therapy, significantly reduces blood pressure and catecholamine levels, and improves inflammatory and endothelial factor levels in patients with gestational hypertension and PE.
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Clinical outcomes of full-endoscopic transforaminal approach via the lumbosacral triangle for the treatment of far lateral L5/S1 disc herniation
Tang Xiaosong; Xiao Qingqing; Wang Wen; Chu Fuming; Li Yue
2026,41(2):206-209 ,DOI: 10.3969/j.issn.1005-3697.2026.02.016
Abstract:
Objective: To assess the feasibility and safety of the full-endoscopic trans-lumbosacral triangle approach for treating L5/S1 far-lateral lumbar disc herniation (FLLDH) and to evaluate its mid-to long-term clinical outcomes. Methods: A single-center retrospective observational study was conducted on 44 patients with L5/S1 far-lateral lumbar disc herniation. All patients underwent full-endoscopic trans-lumbosacral triangle approach surgery. Outcomes were assessed using the Visual Analog Scale (VAS) for low back and lower limb pain, the Oswestry Disability Index (ODI) for lumbar function, and the modified MacNab criteria for excellent/good rates. Radiographic follow-up included dynamic lumbar radiographs, thin-slice lumbar CT, and lumbar MRI. Results: The operative time for the 44 patients ranged from 45 to 73 minutes, with a mean of 58.0 ± 7.71 min. The follow-up period ranged from 35 to 46 months, with a median of 40 months. The low back pain VAS score significantly decreased from 5.41 ± 1.06 preoperatively to 1.57 ± 0.82 at 1-month postoperatively and further reduced to 0.93 ± 0.73 at the final follow-up. The leg pain VAS score decreased from 6.80 ± 1.00 preoperatively to 1.43 ± 0.50 at 1-month postoperatively and 0.84 ± 0.53 at the final follow-up. The ODI improved from 62.93 ± 14.22% preoperatively to 20.58 ± 12.56% at 1-month postoperatively and 6.99 ± 5.47% at the final follow-up. The differences in VAS and ODI scores at both 1-month and final follow-up were statistically significant compared to preoperative values (P < 0.05), and this difference remained statistically significant when compared the final follow-up to the 1-month postoperative assessment (P < 0.05). According to the modified MacNab criteria at the final follow-up, yielding an excellent/good rate of 93.2%. Postoperative thin-slice lumbar CT and MRI confirmed complete removal of the herniated nucleus pulposus, and dynamic lumbar radiographs showed no instability. Conclusion: The full-endoscopic trans-lumbosacral triangle approach demonstrates favorable mid-to long-term clinical outcomes in the treatment of L5/S1 far-lateral lumbar disc herniation.
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Effect of live combined Bifidobacterium triple viable capsules on chronic hepatitis B patients with non-alcoholic fatty liver disease and its impact on carotid atherosclerosis risk based on the gut-liver axis and immune regulation
Wu Limeng; Li Wenting; Kong Linling; Yao Jian
2026,41(2):210-216 ,DOI: 10.3969/j.issn.1005-3697.2026.02.017
Abstract:
Objective: To investigate the effect ofBifidobacterium triple viable capsules on treatment efficacy and carotid atherosclerosis risk in patients with chronic hepatitis B (CHB) complicated by non-alcoholic fatty liver disease (NAFLD), based on the gut-liver axis and immunomodulation mechanisms.Methods: A total of 130 CHB patients with NAFLD were divided into an observation group and a control group according to different treatment methods, with 65 cases in each group. The control group received conventional comprehensive treatment, while the observation group receivedBifidobacterium triple viable capsules in addition to the control group's regimen. After 6 months of continuous intervention, the following indicators were compared between the two groups:Virological response: HBV DNA levels.Liver function: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT), and total bilirubin (TBIL).Liver steatosis and fibrosis: Controlled attenuation parameter (CAP) and liver stiffness measurement (LSM).Intestinal flora indices: Relative abundance ofBifidobacterium andEnterobacteriaceae, and flora α-diversity (Shannon index).Intestinal mucosal barrier indices: Endotoxin (LPS), diamine oxidase (DAO), and intestinal fatty acid-binding protein (I-FABP).Inflammation and immune indices: High-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), CD4+/CD8+ ratio, and regulatory T cell (Treg) proportion.Carotid atherosclerosis risk indices: Carotid intima-media thickness (CIMT), carotid plaque detection rate, maximum area of carotid plaque, and carotid hemodynamic parameters [peak systolic velocity (PSV), end-diastolic velocity (EDV), and resistance index (RI)].Results:Virological & Liver Function: The HBV DNA level in the observation group after treatment was significantly lower than in the control group (P0.05).Gut Microbiota & Barrier: The abundance ofBifidobacterium and the Shannon index were higher in the observation group, while the abundance ofEnterobacteriaceae was lower compared to the control group (P0.05).Conclusion: The addition ofBifidobacterium triple viable capsules to standard antiviral therapy can regulate intestinal flora balance, repair the intestinal mucosal barrier, inhibit systemic inflammatory responses, and improve immune disorders. Consequently, this adjunctive therapy enhances virological response in CHB-NAFLD patients, improves liver function, reduces liver steatosis and fibrosis, and helps delay the early progression of carotid atherosclerosis.
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Application of CBCT detection combined with microscopic root canal technique in evaluating the efficacy of root canal therapy of elderly maxillary second molar
Liu Sijia; Zhang Xu; Xiong Fang
2026,41(2):217-220 ,DOI: 10.3969/j.issn.1005-3697.2026.02.018
Abstract:
Objective: To explore the application of cone-beam computed tomography (CBCT) combined with microscopic root canal technique in the detection of root canals and the evaluation of therapeutic efficacy in elderly patients with maxillary second molars.Methods: A total of 108 elderly patients requiring root canal therapy for maxillary second molars were divided into a control group and an observation group based on different intervention methods, with 54 cases in each group. The control group received panoramic radiography (PAN) combined with conventional root canal therapy, while the observation group was treated with CBCT combined with microscopic root canal therapy. Preoperative diagnosis results, postoperative evaluation results of PAN and CBCT, and therapeutic effects of the two groups were compared.Results: In the observation group, the detection rate of the second mesial canal on the buccal side (MB2) by CBCT was significantly higher than that by PAN (P<0.05). There was a statistically significant difference between the two methods in judging root filling quality (P<0.05), as well as in evaluating periapical healing (P<0.05). The total incidence rate of intraoperative complications and the postoperative pain visual analog scale (VAS) scores in the observation group were lower than those in the control group (P<0.05), and the clinical success rate was higher than that in the control group (P<0.05).Conclusion: The combination of CBCT and microscopic root canal technique can enhance the detection of root canals and the treatment success rate for elderly maxillary second molars, while providing an objective evaluation of therapeutic efficacy.
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Clinical study of Staphylococcus and neisseria tablets combined with Budesonide glycopyrronium bromide and formoterol fumarate inhalation aerosol in the treatment of childhood bronchial asthma
Wang Qian; Shen Qin; Sun Bingxia; Li Fanglin; Zhou Zhonghua
2026,41(2):221-224 ,DOI: 10.3969/j.issn.1005-3697.2026.02.019
Abstract:
Objective: To investigate the efficacy of a combination ofStaphylococcus andNeisseria tablets with Budesonide, glycopyrronium bromide, and formoterol fumarate inhalation aerosol in the treatment of childhood bronchial asthma, as well as its effects on intercellular adhesion molecule-1 (ICAM-1), peripheral blood eosinophils (EOS), and lung function.Methods: A total of 80 pediatric patients with bronchial asthma were enrolled and divided into a control group and a combination group according to different treatment methods, with 40 patients in each group. The control group was treated with Budesonide, glycopyrronium bromide, and formoterol fumarate inhalation aerosol, while the combination group received treatment withStaphylococcus andNeisseria tablets in addition to the control group's regimen. Both groups were treated for 3 months. Treatment efficacy, inflammatory indicators [ICAM-1, interleukin-6 (IL-6) levels], immune function indicators [total immunoglobulin E (IgE) and EOS levels], lung function indicators [maximum voluntary ventilation (MVV), forced vital capacity (FVC), and forced expiratory volume in one second (FEV1)], symptom improvement status [Medical Research Council (MRC) dyspnea scale and Asthma Control Test (ACT) scores], and the incidence of adverse reactions were compared between the two groups.Results: The effective rate of the combination group was higher than that of the control group (P0.05).Conclusion: The combination ofStaphylococcus andNeisseria tablets with Budesonide, glycopyrronium bromide, and formoterol fumarate inhalation aerosol demonstrates significant efficacy in treating bronchial asthma. It effectively reduces the severity of airway inflammatory responses, promotes the recovery of immune function, and improves lung function.
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Effect of growth hormone combined with letrozole treatment on SHBG Th1/Th2 and CTRP9 in patients with polycystic ovary syndrome
Pang Yingyi; Lin Bing; Liang Jieheng; He Jiaxin
2026,41(2):225-229 ,DOI: 10.3969/j.issn.1005-3697.2026.02.020
Abstract:
Objective: To investigate the effects of growth hormone combined with letrozole treatment on serum sex hormone-binding globulin (SHBG), Th1/Th2 cytokines, and C1q tumor necrosis factor-related protein 9 (CTRP9) in patients with polycystic ovary syndrome (PCOS).Methods: A total of 192 patients with PCOS were included in the study and divided into a control group and a combined group according to different treatment schemes, with 96 cases in each group. The control group was treated with letrozole, while the combined group received growth hormone in addition to letrozole. The maximum follicle diameter, endometrial thickness, ovarian volume, interleukin-4 (IL-4), interferon-gamma (IFN-γ), Th1/Th2 cytokine ratios, SHBG, CTRP9, estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P), pregnancy outcomes, and the incidence of adverse reactions were recorded and compared between the two groups.Results: After treatment, the combined group showed significantly higher levels of maximum follicle diameter and endometrial thickness compared to the control group (P0.05).Conclusion: The use of growth hormone combined with letrozole in the treatment of polycystic ovary syndrome can regulate immune imbalance by shifting cytokine status, enhance ovarian reserve function, and improve levels of SHBG, CTRP9, and sex hormones. This combination therapy helps improve ovulation and pregnancy rates in patients and demonstrates a good safety profile.
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Differential diagnosis of prostate cancer and benign prostatic hyperplasia by intravoxel incoherent motion model histogram parameters
Zhu Yifang; Wu Hengli; Xu Chunsheng; Wang Anqin
2026,41(2):230-234 ,DOI: 10.3969/j.issn.1005-3697.2026.02.021
Abstract:
Objective: To analyze the differential diagnosis efficiency of intravoxel incoherent motion (IVIM) model histogram parameters for prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Methods: The clinical data of 90 patients with clinically suspected PCa but without hormone therapy or radiotherapy who underwent multiparametric MRI (mpMRI) examination were retrospectively analyzed. The enrolled patients were classified into the PCa group and the BPH group based on pathological results. Histogram parameters such as the pseudo-diffusion coefficient (), quantitative diffusion coefficient (D), apparent diffusion coefficient (ADC), and perfusion fraction (f) were obtained, and differences in these parameters were compared. The diagnostic efficiency of each parameter in differentiating PCa from BPH was analyzed by drawing receiver operating characteristic (ROC) curves. Results: The mean value, 75th percentile, 90th percentile, and maximum value of D in the PCa group were lower than those in the BPH group (P<0.05), and the minimum value and 10th percentile of D were also lower (P<0.05). The mean value, main percentiles, and kurtosis of f were lower in the PCa group than in the BPH group (P<0.05). The minimum value, main percentiles, and maximum value of ADC in the PCa group were lower, while kurtosis was higher compared to the BPH group (P<0.05). The areas under the curve (AUCs) of the mean value of D, minimum value of D*, mean value of f, and 50th percentile of ADC were significantly higher (P<0.05). Conclusion: IVIM model histogram parameters have significant advantages in identifying PCa and BPH, providing a valuable reference basis for clinical diagnosis.
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Efficiency of transcranial Doppler ultrasound flow imaging combined with aEEG parameters on predicting the prognosis of acute stroke
2026,41(2):235-238 ,DOI: 10.3969/j.issn.1005-3697.2026.02.022
Abstract:
Objective: To explore the efficiency of transcranial Doppler ultrasound flow imaging (TCD-FI) combined with amplitude-integrated electroencephalogram (aEEG) parameters in predicting the prognosis of acute stroke. Methods: The data of 121 patients with acute stroke were retrospectively analyzed. Prognosis was evaluated by the modified Rankin Scale (mRS). Patients with an mRS score of less than 2 points were incorporated into the good prognosis group (n=87), and patients with an mRS score ≥ 2 points were enrolled in the poor prognosis group (n=34). General data, TCD-FI cerebral hemodynamic parameters [peak systolic blood flow velocity (Vs), end-diastolic blood flow velocity (Vd), resistance index (RI)], and aEEG brain electrical activity (upper and lower boundary voltages) were compared between the two groups. A receiver operating characteristic (ROC) curve was utilized to analyze the predictive efficiency of TCD-FI combined with aEEG parameters on the prognosis of acute stroke. Pearson correlation analysis was used to analyze the correlation between each parameter and the mRS score. Results: Statistical differences were found in age, infarction size, and degree of vascular stenosis between the two groups (P<0.05). The Vs, Vd, upper boundary voltage, and lower boundary voltage in the poor prognosis group were lower than those in the good prognosis group, while the RI was higher compared to the good prognosis group (P<0.05). ROC curve analysis revealed that the area under the curve (AUC), sensitivity, and specificity of TCD-FI combined with aEEG parameters were 0.873, 52.94%, and 100%, respectively. Correlation analysis showed that Vs, Vd, upper boundary voltage, and lower boundary voltage all exhibited negative correlations with the mRS score (P<0.05), and RI showed a positive correlation with the mRS score (P<0.05). Conclusion: TCD-FI combined with aEEG parameters has high predictive efficiency for the prognosis of acute stroke and can provide a reference for clinical treatment.
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Correlation between ABCG1 gene polymorphism and lower extremity atherosclerosis in the elderly
Wang Min; Wu BiHua; Luo Han; Zhou KeJing
2026,41(2):239-243+248 ,DOI: 10.3969/j.issn.1005-3697.2026.02.023
Abstract:
Objective: To investigate the correlation between ATP binding cassette transporter G1 (ABCG1) polymorphisms and lower extremity atherosclerosis disease (LEAD) in individuals aged over 60. Methods: 200 hospitalized patients with LEAD confirmed by vascular ultrasound (case group) and 66 age-matched healthy controls (control group) were enrolled. ABCG1-rs1378577, rs225374, and rs57137919 polymorphisms were analyzed via TaqMan probe assay. The correlation between ABCG1 polymorphism and LEAD in elderly individuals was analyzed. Logistic regression was used to analyze the risk factors of LEAD in elderly patients. Results: For the ABCG1-rs1378577 locus: Genotype distribution in cases was GG > GT > TT, showing statistical significance (P GG > CC, without statistical significance (P>0.05). For the ABCG1-rs57137919 locus: genotype distribution in the case group ranked GG > AG > AA, showing statistical significance (P<0.05). The AA genotype exhibited a negative correlation with disease incidence (r=-0.172, P<0.05), while Logistic regression identified that the GG genotype increases the risk of disease (OR=4.168, P<0.05). Conclusion: ABCG1-rs1378577 and rs57137919 are correlated with LEAD in the elderly, while ABCG1-rs225374 shows no significant correlation.
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The clinical predictive value of T lymphocyte subset composition changes and mitochondrial damage in patients with bipolar disorder
2026,41(2):244-248 ,DOI: 10.3969/j.issn.1005-3697.2026.02.024
Abstract:
Objective: To investigate the changes and clinical significance of T lymphocyte subsets and mitochondrial damage in patients with bipolar disorder (BD). Methods: 102 BD patients were retrospectively selected as the observation group. According to the evaluation results of the Clinical Global Impressions-Severity scale at admission, the patients were classified into a mild group (n=41), moderate group (n=35), and severe group (n=26). All patients received standardized treatment with quetiapine fumarate tablets combined with amitriptyline hydrochloride tablets for 8 weeks after admission, and were categorized into an effective treatment group (n=81) and an ineffective treatment group (n=21) based on treatment response results. 90 healthy subjects undergoing physical examination were included in the control group. T lymphocyte subsets (percentage of CD45+ lymphocytes, percentage of CD3+ T cells) and mitochondrial damage indexes of helper T lymphocytes (Th) and cytotoxic T lymphocytes (Ts) were detected and compared. A receiver operating characteristic (ROC) curve was drawn to analyze the predictive value of related indicators. Results: Compared with the control group, the percentages of CD45+ lymphocytes and CD3+ T cells in the observation group were lower, while the positive rates of Th and Ts mitochondrial damage indexes were higher (P<0.05). Compared with the mild group, the percentage of CD45+ lymphocytes and percentage of CD3+ T cells in the moderate group and the severe group were lower in turn, while the positive rate of Th cell mitochondrial damage index and positive rate of Ts cell mitochondrial damage index were higher in turn (P<0.05). ROC curve analysis revealed that the areas under the curves (95% CI) of the above indicators for predicting severe BD were 0.672 (0.536~0.807), 0.628 (0.492~0.763), 0.706 (0.595~0.818), and 0.700 (0.586~0.815) respectively, and the area under the curve (95% CI) of the combined prediction of the four indicators was higher at 0.846 (0.761~0.931). The percentage of CD45+ lymphocytes and percentage of CD3+ T cells in the ineffective treatment group were lower compared with those in the effective treatment group, while the positive rates of Th and Ts cell mitochondrial damage indexes were higher (P<0.05). ROC curve analysis showed that the areas under the curves (95% CI) of the above indicators for predicting BD treatment ineffectiveness were 0.892 (0.803~0.982), 0.858 (0.759~0.956), 0.785 (0.691~0.879), and 0.773 (0.672~0.875), respectively, and the combined prediction value of the four indicators was higher, with an area under the curve (95% CI) of 0.981 (0.727~0.989). Conclusion: BD patients have immune dysfunction. T lymphocyte subsets and mitochondrial damage indexes can be used as effective indicators to evaluate the disease condition and treatment response.
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Relationship of serum Cys-C retinol-binding protein urine microalbumin to creatinine ratio and disease severity in patients with T2DM
2026,41(2):249-251 256 ,DOI: 10.3969/j.issn.1005-3697.2026.02.025
Abstract:
Objective: To explore the relationship between serum cystatin C (Cys-C), retinol-binding protein (RBP), urine microalbumin-to-creatinine ratio (ACR), and disease severity in patients with type 2 diabetic nephropathy (T2DN). Methods: A total of 103 patients with T2DN were enrolled as the T2DN group. According to the excretion rate of urinary albumin, they were divided into the early stage group (n=36), clinical stage group (n=55), and uremia group (n=12). A total of 105 patients with type 2 diabetes mellitus (T2DM) during the same period were enrolled as the T2DM group. The levels of serum Cys-C, RBP, and ACR in the T2DN group and T2DM group were detected and compared, and their relationship with disease severity was analyzed. The diagnostic efficiency of Cys-C, RBP, ACR, and combined detection for the severity of T2DN was analyzed by ROC curves. Results: The levels of serum Cys-C, RBP, and ACR in the T2DN group were higher than those in the T2DM group (P<0.05). The levels of Cys-C, RBP, and ACR in the clinical stage group and uremia group were higher than those in the early stage group (P<0.05). Serum Cys-C, RBP, and ACR were positively correlated with disease severity (P<0.05). The AUC of Cys-C combined with RBP and ACR in the diagnosis of T2DN in the clinical stage and uremia stage was 0.934, greater than that of a single index (P<0.05). Conclusion: The changes of Cys-C, RBP, and ACR are positively correlated with the severity of T2DN, and combined detection of the three indexes has higher diagnostic efficiency.
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Effect of Teach-back health education combined with graded exposure therapy on postoperative kinesiophobia psychological resilience and post-traumatic growth in patients with lung cancer
Zhou Huanhuan; Huang Na; Liu Xue; Zhang Liping
2026,41(2):252-256 ,DOI: 10.3969/j.issn.1005-3697.2026.02.026
Abstract:
Objective: To investigate the effect of Teach-back health education combined with graded exposure therapy on postoperative kinesiophobia, psychological resilience, and post-traumatic growth in patients with lung cancer. Methods: A total of 102 patients who underwent lung cancer surgery were selected and divided into an intervention group (n=51) and a control group (n=51) according to the different intervention methods. The control group received routine care, while the intervention group received additional Teach-back health education and graded exposure therapy. The intervention lasted for 4 weeks, after 4 weeks the levels of kinesiophobia (Tampa Scale of Kinesiophobia, TSK), psychological resilience (Connor-Davidson Resilience Scale, CD-RISC), and post-traumatic growth (Chinese-Posttraumatic Growth Inventory, C-PTGI) were evaluated in both groups. Results: After the intervention, the intervention group showed lower scores in all dimensions of the TSK—including kinesiophobia, functional disorder, activity avoidance, and risk perception—as well as in the total score compared to the control group (P<0.05). The intervention group demonstrated higher scores in all dimensions of the CD-RISC—including strength, tenacity, and optimism—compared to the control group (P<0.05). The intervention group exhibited higher scores in all dimensions of the C-PTGI—such as relationship with others, personal strength, spiritual change, appreciation of life, and new possibilities—as well as in the total score compared to the control group (P<0.05). Conclusion: The combined intervention of Teach-back health education and graded exposure therapy has positive clinical value in reducing postoperative kinesiophobia, enhancing psychological resilience, and promoting post-traumatic growth in patients with lung cancer.
Basic Medical Research
Clinical medical research
nursing
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DOK1 recombinant adenovirus enhances insulin sensitivity of BRL-3A cells through DOK1/AKT/SREBP1c signalling pathway
Cai Xiaoling; Wang Chunlan; Ha Xiaoqin;
2025,40(6):681-685 ,DOI: 10.3969/j.issn.1005-3697.2025.06.001
Abstract:
Objective:To investigate the mechanism of DOK1 recombinant adenovirus(Ad-DOK1)on the regulation of insulin sensitivity in palmitic acid(PA)-induced insulin-resistant hepatocytes(BRL-3A).Methods:The control,solvent and model groups were treated with normal medium,ethanol and palmitic acid to establish a cellular insulin resistance model of BRL-3A cells and to iden-tify the cellular model using cellular glucose uptake assay.The control,null and adenovirus DOK1 overexpression groups were transfect-ed with normal serum,negative virus and Ad-DOK1(51257-1)in insulin-resistant BRL-3A cells,and the transfection efficiency of ade-novirus was detected by flow cytometry,and the Western blot method was used to detect DOK1,IRSI,IRSII,p-PI3K,PI3K,p-AKT,AKT,SREBP1c protein expression levels.Results:Palmitic acid induction for 6 hours resulted in a decrease in intracellular 2-deoxyglu-cose(P＜0.05),down-regulation of DOK1 protein expression level(P＜0.05),no significant change in IRSI and IRSII protein ex-pression level(P>0.05),no change in p-PI3K/PI3K ratio(P>0.05),down-regulation of p-AKT/AKT ratio(P＜0.05),and SREBP1c protein expression expression levels were upregulated(P＜0.05).After transfection of cells with DOK1 recombinant adeno-virus of optimal MOI 60,compared with the normal control group,intracellular 2-deoxyglucose was elevated(P＜0.05),DOK1 protein expression level was up-regulated(P＜0.05),there was no significant change in the expression level of IRSI and IRSII proteins(P>0.05),no significant change in the p-PI3K/PI3K ratio(P>0.05),the p-AKT/AKT ratio was upregulated(P＜0.05),and the ex-pression level of SREBP1c protein expression was downregulated(P＜0.05).Conclusion:Ad-DOK1 may enhance insulin sensitivity of BRL-3A cells by regulating AKT and SREBP1c.
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Development and validation of a malignancy risk prediction model for pul-monary nodules based on multivariable analysis and machine learning
Hu Xin; Jiang Yongjie; Shilun Guang; Heibieluo; Huang Yuyan; Jiang Li;
2025,40(6):686-692 712 ,DOI: 10.3969/j.issn.1005-3697.2025.06.002
Abstract:
Objective:To develop a robust and widely applicable predictive model to improve the accuracy of diagnosing the ma-lignancy risk of pulmonary nodules.Methods:This study retrospectively collected clinical data from 1,414 patients with pulmonary nod-ules diagnosed and treated at the Affiliated Hospital of North Sichuan Medical College and the Guang'an People's Hospital.Meta-analy-sis and Least Absolute Shrinkage and Selection Operator(LASSO)regression were used to identify predictors related to the malignancy risk of pulmonary nodules.These factors were further optimized by multivariable Logistic regression(LR)to determine key features.Based on these features,8 machine learning models were constructed and evaluated for performance using Receiver Operating Character-istic(ROC)curves,calibration curves,and Decision Curve Analysis(DCA)in the training set and internal validation set.The best-performing model was used to develop a nomogram for risk stratification of patients.Results:Through the combined screening process of Meta-analysis,LASSO regression,and multivariable LR,10 key predictive factors were identified and integrated into eight different ma-chine learning models.Model evaluation demonstrated that the LR model performed best,achieving an Area Under the Curve(AUC)of 0.843 in the internal validation cohort.Additionally,the nomogram derived from this model exhibited strong predictive ability in the ex-ternal validation cohort,with an AUC of 0.770.Risk scores calculated from the nomogram stratified patients into four risk groups,with malignancy rates ranging from 0%in the low-risk group to 100%in the very high-risk group.Conclusion:The prediction model devel-oped in this study effectively assesses the malignancy risk of pulmonary nodules,providing a valuable risk stratification tool for clinical use.
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Effect and mechanism of miR-139-3p targeting SPP1 on cisplatin resist-ance in A549 lung cancer cells
2025,40(10):1225-1229 1248 ,DOI: 10.3969/j.issn.1005-3697.2025.10.001
Abstract:
Objective:To explore the effect and mechanism of miR-139-3p targeting secreted phosphoprotein 1(SPP1)on cis-diamminedichloroplatinum(DDP)in A549 lung cancer cells.Methods:A cisplatin(DDP)-resistant A549/DDP cell model was established via gradient concentration induction,validated by resistance index(IR=3.877)and IC50 determination.The re-sistant cells were divided into five groups:blank control,negative control,miR-139-3p mimic,SPP1 overexpression,and SPP1 knockdown.Multi-platform analyses were performed,including qPCR(miR-139-3p and SPP1 expression),Western blot(SPP1,p-AKT,and total AKT protein levels),CCK-8(proliferation and drug sensitivity),flow cytometry(apoptosis),and du-al-luciferase reporter assays(miR-139-3p/SPP1 interaction).Results:The cisplatin-resistant A549/DDP cells exhibited a signifi-cantly higher IC50 value compared to parental A549 cells(P<0.05),with a resistance index(IR)of 3.877.Quantitative PCR(qPCR)analysis revealed that miR-139-3p expression was markedly upregulated in the miR-139-3p mimic group versus the negative control group(P<0.05).Relative to the miR-139-3p mimic group,SPP1 mRNA levels were significantly elevated in the SPP1-overex-pression group and reduced in the SPP1-knockdown group(P<0.05).Western blotting confirmed corresponding changes in SPP1 pro-tein expression across these groups(P<0.05).CCK-8 assays demonstrated that miR-139-3p overexpression enhanced the proliferation inhibition rate and reduced the IC50 value compared to the negative control(P<0.05),whereas SPP1 overexpression reversed these effects,lowering the inhibition rate and increasing IC50(P<0.05).Compared with the miR-139-3p blank control,the luciferase activity of the wild-type SPP13'UTR luciferase reporter plasmid binding to miR-139-3p analog decreased(P<0.05),and the difference in the luciferase activity of the mutant SPP13'UTR luciferase reporter plasmid binding to miR-139-3p analog was not statistically significant(P>0.05).Compared with the negative control group,the protein expression level of p-AKT in miR-139-3p control group was signifi-cantly decreased(P<0.05),compared with the miR-139-3p control group and SPP1 knockdown group,the protein expression level of p-AKT in SPP1 overexpression group was significantly increased(P<0.05).Conclusion:Overexpression of miR-139-3p or knockdown of SPP1 could inhibit the proliferation of A549/DDP cells,enhance their sensitivity to cisplatin,and the mechanism may be related to the AKT signaling pathway.
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The causal relationship between sleep disorders and gastric ulcer incidence based on two-sample Mendelian randomization
2025,40(6):693-698 ,DOI: 10.3969/j.issn.1005-3697.2025.06.003
Abstract:
Objective:To investigate whether there was a potential two-way causal relationship between sleep disorder(SD)and the incidence of gastric ulcer(GU)by two sample Mendelian randomization(MR)analysis.Methods:The data sets of exposure and outcome were obtained from IEU database,from which suitable Single Nucleotide Poly Morphisms(SNPs)were selected as instrumental variables(IVs),and then inverse variance weighting(IVW),MR-Egger regression,weighted median(WME),simple model and weigh-ted model were used for two-way MR Analysis.Cochran's Q test and MR-Egger intercept test were used for sensitivity analysis.Results:The IVW method showed that there was a significant unidirectional causal relationship between SD and the incidence of GU.When SD were used as exposure factors,the positive MR Analysis had significant statistical significance(OR=1.009,95%CI:1.004～1.015,P=0.001).When GU used as an exposure factor,reverse MR Analysis was not statistically s-ignificant(OR=1.197,95%CI:0.425～3.371,P=0.734).Further sensitivity analysis showed no significant heterogeneity and horizontal pleiotropy.Conclusion:Mendelian randomization analysis found that there is a potential one-way causal relationship between SD and the risk of GU,and SD are risk factors for GU.
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Effect of esketamine combined with erector spinae plane block on the qual-ity of recovery in patients after thoracoscopic lobectomy under general an-esthesia
Liu Tongxin; Hou Yongchao; He Peng; Zhang Zhenghui; Zhou Shuzhi;
2025,40(6):708-712 ,DOI: 10.3969/j.issn.1005-3697.2025.06.006
Abstract:
Objective:To investigate the effect of intravenous esketamine combined with erector spinae plane block(ESPB)on the postoperative recovery quality in patients undergoing thoracoscopic lobectomy with general anesthesia.Methods:A total of 97 pa-tients who underwent thoracoscopic lobectomy were divided into two groups:group K(n=49)and group C(n=48),according to whether or not they were given esketamine.Patients in K group were given slow intravenous injection of 0.2 mg/kg ketamine after anes-thesia induction and 5 minutes before surgical skin incision,and patients in group C received intravenous injection of physiological sa-line equivalent to group K drugs.Comparison of general indicators,quality of recovery at 24 h postoperatively[40-item Quality of Re-covery Rating Scale(QoR-40)score],cellular immune function before and after 24 h of treatment(NK cells,CD4+T cells,and CD8+T cells),hemodynamics at each time point,and the occurrence of postoperative adverse events between the two groups.Results:Intrao-perative remifentanil dosage was lower in group K than in group C(P＜0.05).The overall QoR-40 score,as well as the subscores for pain,emotional state,and physical comfort in group K were higher than those in group C at 24 hours postoperatively(P＜0.05).Addi-tionally,levels of NK cells,CD4+T cells,and CD8+T cells were higher in the group K than those in group C at 24 hours postoperative-ly(P＜0.05).There were no statistically significant differences in hemodynamics and total incidence of adverse effects between the two groups(P>0.05).Conclusion:The intravenous application of esketamine combined with ESPB for thoracoscopic lobectomy under general anesthesia reduces intraoperative remifentanil dosage,improves the quality of postoperative recovery and improves postoperative cellular immune function in patients without increasing adverse effects.
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Correlation between serum brain-derived neurotrophic factor and mono-cyte to HDL-C ratio levels and post-stroke cognitive impairment in pa-tients with acute ischemic stroke
Shen Yongling; Fang Jianwei; Cheng Fei; Sang Xiaoyi; Meng Jun; Ma Cuihong; Gohong Country;
2025,40(5):624-627 ,DOI: 10.3969/j.issn.1005-3697.2025.05.018
Abstract:
Objective:To investigate the correlation between serum brain-derived neurotrophic factor(BDNF),monocyte to HDL-C ratio(MHR)and post-stroke cognitive impairment(PSCI)in patients with acute ischemic stroke(AIS).Methods:96 patients with acute AIS were collected and divided into PSCI group(n=39,<26 points)and non-PSCI group(n=57,≥26 points)accord-ing to the Montreal Cognitive Assessment(MoCA)score at 3 months after stroke.The clinical data such as serum BDNF and MHR lev-els at admission were statistically compared between the two groups,and the receiver operating characteristic(ROC)curve of BDNF and MHR levels in predicting PSCI was drawn.Results:Compared with the non-PSCI group,the PSCI group was older,had shorter years of education,had a higher proportion of hypertension,and had lower serum BDNF and higher MHR levels,the differences were signifi-cant(P<0.05).Logistic regression analysis showed that age(OR=1.530),hypertension(OR=1.335),serum BDNF level(OR=0.755)and MHR(OR=1.645)were risk factors for PSCI in AIS patients(P<0.05).ROC curve analysis showed that the AUC of BDNF and MHR levels in predicting PSCI were 0.833 and 0.745,respectively,and the AUC of the combined diagnosis of the two was 0.882,which was greater than that of a single index(P<0.05).Conclusion:Serum BDNF and MHR are closely related to PSCI in AIS patients.Both indicators can predict the occurrence of PSCI,and the combined detection of the two can improve the predictive abili-ty.
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Value of lung ultrasound score combined with peripheral blood procalci-tonin,C-reactive protein and interleukin-6 levels on evaluating the disease severity and prognosis of patients with acute respiratory distress syn-drome
Wang Xiyu; Liao Rui; Wang Zhong;
2025,40(10):1244-1248 ,DOI: 10.3969/j.issn.1005-3697.2025.10.005
Abstract:
Objective:To explore the value of lung ultrasound(LUS)score combined with peripheral blood procalcitonin(PCT),C-reactive protein(CRP)and interleukin-6(IL-6)levels on evaluation of disease severity and prognosis of patients with acute respiratory distress syndrome(ARDS).Methods:109 patients with ARDS were selected.The disease severity and progno-sis of patients were grouped according to the Berlin criteria for ARDS.The baseline data were compared among mild group(n=24),moderate group(n=55)and severe group(n=30).According to the prognosis,they were divided into a survival group(n=68)and a death group(n=41).The differences in LUS score,peripheral blood PCT,CRP and IL-6 levels among the three groups and patients with different prognoses were analyzed,and the evaluation value of the above indexes on the disease severity and prognosis was analyzed.Results:There were statistical differences in prognosis and APACHE Ⅱ score among the three groups(P<0.05).The survival rate of patients in severe group was lower than that in moderate group(P<0.05),and the rate in moderate group was lower than that in mild group(P<0.05).The APACHE Ⅱ score of patients in severe group was higher than that in moderate group(P<0.05),and the score in moderate group was higher than that in mild group(P<0.05).The LUS score and peripheral blood PCT,CRP and IL-6 levels revealed statistical differences among ARDS patients with different disease severities(P<0.05).The severe group was higher than the moderate group(P<0.05),and the moder-ate group was higher than the mild group(P<0.05).Spearman analysis showed that LUS score and peripheral blood PCT,CRP and IL-6 in ARDS patients were positively correlated with disease severity(P<0.05).The LUS score,PCT,CRP and IL-6 in death group were higher than those in survival group(P<0.05).After Logistic regression analysis,it was found that A-PACHE Ⅱ score,LUS score,PCT,CRP,IL-6 and disease severity were the influencing factors of prognosis in patients with ARDS(P<0.05).ROC curve results showed that the areas under the ROC curves of LUS score,peripheral blood PCT,CRP and IL-6 and combined detection for predicting the death prognosis in ARDS patients were 0.778,0.781,0.864,0.856 and 0.982,and the sensitivities were 75.61％,78.05％,90.24％,80.49％and 92.68％,respectively.The combined detection value was higher.Conclusion:LUS score and peripheral blood PCT,CRP and IL-6 levels can effectively evaluate the disease severity of ARDS patients,and have reliable predictive value for prognosis of patients.
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Primary study of vortioxetine in inhibiting proliferation and migration of esophageal cancer cells
Roman; Ma Qiang; Zhong Xiaowu; Liao Zhongbo; Xu Lei; Guo Xiaolan;
2025,40(5):545-550 ,DOI: 10.3969/j.issn.1005-3697.2025.05.001
Abstract:
Objective:To investigate the effects and mechanisms of vortioxetine on the proliferation and migration of esophageal squamous cell cancer.Methods:CCK-8 assay was used to detect the viability of Eca109 and KYSE150 cells after treated with vortioxe-tine at different concentrations(0,2,4,6,8,10 μmol/L).Moreover,transwell and scratch assays were employed to evaluate the migra-tion of ESCC cells,plate colony formation assay was used to detect the proliferation.The differentially expressed genes were screened by TCGA database,AutoDock software was used to predict the binding of vortioxetine and cyclooxygenase-2(COX-2)protein.Further-more,the expression of COX-2 mRNA and protein were measured by RT-qPCR and Western blot,respectively.Results:The cell viabil-ity of Eca109 and KYSE150 decreased in a concentration-dependent manner after treatment with different concentrations of vortioxetine(P<0.05),and the semi-inhibitory concentrations(IC50)were 5.086 μmol/L and 5.179 μmol/L,respectively.Compared with the control group,the cell proliferation and migration of the vortioxetine group were reduced(P<0.05).In addition,vortioxetine could in-hibit the expression of COX-2 at mRNA and protein level(P<0.05).Conclusion:Vortioxetine inhibits the proliferation and migration of esophageal cancer cells probablly through COX-2 down-regulation.
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Application value of contrast-enhanced CT histogram parameters in mor-tality risk assessment of severe acute pancreatitis
Taodi; Zhao Zisheng; Chen Junhui; Zhang Xiaoming;
2025,40(5):562-568 ,DOI: 10.3969/j.issn.1005-3697.2025.05.004
Abstract:
Objective:To optimize contrast-enhanced computed tomography(CECT)image quality using Non-Local Means(NLM)denoising technology and construct a mortality risk prediction model for severe acute pancreatitis(SAP)patients based on his-togram parameters.Methods:Retrospective analysis of clinical,laboratory,and CECT data of 74 patients diagnosed with SAP.NLM de-noising was applied to arterial and portal venous phase images at different coefficient levels.Three-dimensional volumes of interest were manually delineated in pancreatic parenchyma on original and denoised images,followed by histogram parameter extraction.After univa-riate Logistic regression feature selection,binary Logistic regression models were built to predict SAP mortality risk and compared with clinical models and conventional CT models.Results:A total of 74 patients with SAP,of whom 27(36.5%)died.After applying NLM-based noise reduction to arterial phase and portal venous phase CECT images,8 histogram prediction models were constructed under dif-ferent noise coefficient levels.There was no significant difference in predictive performance among these histogram models(P＞0.05).Compared to conventional CT models and clinical models,the histogram models demonstrated higher accuracy in predicting mortality risk(P=0.009,P=0.048).The predictive performance of the clinical model and the conventional CT model was similar(P=0.699).Conclusion:CECT histogram parameters show significant clinical value in predicting mortality risk in SAP patients,maintaining stable performance under different noise coefficient levels after NLM denoising.Their noise robustness and quantitative a-nalysis advantages compensate for the limitations of conventional CT.
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Effect of evolocumab in patients with early neurological deterioration after noncardiac acute ischemic stroke
Wang Xiaogan; Fan Ying; Fan Lijuan; Zhou Lifei; Gao Yongchao; Liu Minxiao;
2025,40(5):593-596 ,DOI: 10.3969/j.issn.1005-3697.2025.05.011
Abstract:
Objective:To examine the impact of the Evolocumab on early neurological deterioration(END)in patients with non-cardioembolic acute ischemic stroke(NCIS).Methods:A total of 110 patients diagnosed with NCIS and END were recruited as re-search subjects.All patients received standardized and systematic treatment protocols.Based on different therapeutic regimens,the pa-tients were categorized into two groups:the conventional group(n=55),which received standard dual antiplatelet therapy,and the ob-servation group(n=55),which received evolocumab in addition to the standard treatment regimen.The clinical efficacy,cerebral blood flow perfusion indicators[resistance index(RI),cerebral blood volume(CBV),and mean blood flow(Qmean)],levels of nerve func-tion reconstruction factors[nerve growth factor(NGF),gamma aminobutyric acid(GABA),and brain-derived neurotrophic factor(BDNF)],lipid metabolism indicators[apolipoprotein B(ApoB),low-density lipoprotein cholesterol(LDL-C)],and incidence of ad-verse reactions were compared between the two groups.Results:The total clinical efficacy rate in the observation group was higher than that in the conventional group(98.18%vs.85.45%,P<0.05).Post-treatment,the RI value in the observation group was lower than that in the conventional group(P<0.05).Additionally,post-treatment serum levels of CBV and Qmean,as well as NGF,GABA,and BDNF,were higher in the observation group compared to the conventional group(P<0.05).Conversely,post-treatment serum levels of Lp(a),ApoB,and LDL-C,and the total incidence of adverse reactions were lower in the observation group compared to the conventional group(P<0.05).Conclusion:Evolocumab enhances cerebral blood perfusion and facilitates neurological function recovery in NCIS patients with early END.Additionally,it regulates lipid metabolism and decreases the incidence of adverse reactions.
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Relevant studies that NETs promote the secretion of inflammatory media-tors by alveolar-type epithelial cells
Xu Suqing; Tian Jiamei; Lu Hongyan;
2025,40(5):557-561 ,DOI: 10.3969/j.issn.1005-3697.2025.05.003
Abstract:
Objective:To explore the role of neutrophil extracellular traps(NETs)in promoting the secretion of inflammatory mediators by alveolar type epithelial cells(AECII).Methods:In 30 7 d born SD rats,blood was drawn from neutrophils(NE)and di-vided into control(control group),LPS and LPS+DNase groups according to different treatment methods.NETs formation was detec-ted by immunofluorescence in vitro.NETs and AECII were co-cultured,and divided into control group(control group),NETs group and NETs+DNase group according to cell suspension incubation method.AECII apoptosis was detected by flow method,the content of free deoxyribonucleic acid(cf-DNA)in coculture supernatants in each group was detected by fluorescence microarray,and the coculture su-pernatants of interleukin 6(IL-6),interleukin 8(IL-8)and tumor necrosis factor(TNF-α)in each group were determined using ELISA kit.And,the correlation between cf-DNA and IL-6,IL-8,and TNF-α was evaluated using Pearson correlation analysis.Results:According to immunofluorescence,mouse citrullinated histone H3(CitH3)and myeloperoxidase(MPO)were found in neutrophil in LPS and LPS+DNase group,CitH3 and MPO levels in LPS and LPS+DNase groups were higher than in the control group(P<0.05),and CitH3 and MPO in LPS+DNase group were lower than those in LPS group(P<0.05).After AECII apoptosis with NE and NETs,there was no significant difference between early and late apoptosis in the three groups(P＞0.05).The levels of cf-DNA,IL-6,IL-8,and TNF-α in NETs group and NETs+DNase group were higher than those of the control group(P<0.05),NETs+DNase group was lower than NETs group(P<0.05).The content of cf-DNA in cell culture supernatant was positively correlated with the levels of IL-6,IL-8,and TNF-α(r=0.432,0.387,0.326,P<0.05).Conclusion:AECII with NETs in the supernatants with NE,NETs could promote the secretion of inflammatory mediators such as IL-6,IL-8,and TNF-α.Moreover,the more NETs are generated,the more inflammatory mediators such as IL-6,IL-8,and TNF-α are secreted.
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Distribution of peripheral blood B cell subsets in patients with systemic lupus erythematosus and their clinical applications
2025,40(10):1285-1290 ,DOI: 10.3969/j.issn.1005-3697.2025.10.014
Abstract:
Objective:To analyze the distribution of peripheral blood B cell subsets in patients with systemic lupus erythe-matosus(SLE)and their clinical applications.Methods:52 SLE patients(SLE group),36 healthy controls(HC group)and 30 rheumatoid arthritis(RA group)patients were included.The percentage of B1,FOB,Breg and ABC cells in total B cells was measured by Flow cytometry.Diagnostic efficacy of individual and combined B cell subsets for SLE was evaluated via ROC curves.Clinical data on SLE activity score(SLEDAI),erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),com-plement C3 and C4,immunoglobulin,and various autoantibodies from SLE patients were collected,and the correlations between the subsets percentage and these indicators were analyzed.Results:Compared to HC group,the percentage of B1,Breg,and ABC were higher in SLE group(P<0.05)and the percentage of FOB was lower(P<0.05).The percentage of B1,and Breg in SLE group were obviosly higher than those in RA group(P<0.05)and FOB was lower(P<0.05).There was no statistically sig-nificant difference in the proportion of cells in each subgroup between the RA group and the HC group(P>0.05).ROC curve analysis showed that all B cell subgroups had a certain diagnostic efficacy for SLE,and the combination of multiple subgroups had a higher diagnostic efficacy for SLE.The area under the curve(AUC)of 11 combinations for diagnosing SLE was 0.756～0.892(P<0.05),with(ABC+B1+Breg+FOB)combined was highest.Results of Assay of correlations showed that the per-centage of ABC cells positively correlated with the level of ESR(P<0.05,r=0.403).The percentage of Breg cells negatively correlated with the level of C3(P<0.05,r=-0.421)and positively correlated with the level of anti-dsDNA(P<0.05,r=0.488),anti-M2(P<0.05,r=0.604),SLEDAI(P<0.05,r=0.353).ABC and FOB were negatively correlated in the SLE group(P<0.05,r=-0.571),while there was no correlation between the two in the HC and RA groups(P>0.05).Conclu-sion:There are differences in the distribution of B cell subsets between SLE patients and the normal population.The combina-tion of single or multiple indicators has good diagnostic efficacy for SLE,and the distribution of subsets is correlated with multi-ple clinical and laboratory indicators,suggesting that it may have value in disease diagnosis and disease assessment.
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Effects of iTBS and 1 Hz rTMS on depressive symptoms and cognitive function in elderly patients with depression
Zhou Xueying; Xu Deyi; Shang Fangjie;
2025,40(6):703-707 ,DOI: 10.3969/j.issn.1005-3697.2025.06.005
Abstract:
Objective:To explore the effects of intermittent theta burst magnetic stimulation(iTBS)and 1 Hz repetitive tran-scranial magnetic stimulation(rTMS)on depressive symptoms and cognitive function in elderly patients with depression.Methods:Eld-erly patients with depression were divided into iTBS group(n=30)and 1 Hz rTMS group(n=43)according to the treatment regi-men.On the basis of antidepressant treatment for both groups,the left dorsolateral prefrontal lobe of the iTBS group was given a stimula-tion frequency of 50 Hz from the internal frequency,5 Hz from the inter-frequency and stimulation strength of 110%RMT,with a stimu-lation time of 2 s,an intermission of 8 s,and a repetition of 20 times,with a total of 600 pulses lasting for 200 s.The 1 Hz rTMS group was given a stimulation frequency of 1.0 Hz and a stimulation intensity of 110%RMT in the dorsolateral right prefrontal lobe,and was treated for 20 min per day,with a total of 1,200 pulses per treatment.Patients in both groups were treated 5 times per week at the same fixed time for 4 weeks for a total of 20 treatments.The Hamilton Depression Scale 24(HAMD-24),Geriatric Depression Scale(GDS),Beck Suicidal Ideation Inventory-Chinese Version(BSI-CV),Wisconsin Card Sorting Test,and Part A of the Connection Test were used for assessment before and after treatment,and adverse reactions during treatment were recorded.Results:With the prolongation of treat-ment time,the HAMD-24 and GDS scores of the two groups gradually decreased(P＜0.05),and the HAMD-24 and GDS scores of the iTBS group were lower than those of the 1 Hz rTMS group,and the difference was statistically significant(P＜0.05).After4 weeks of treatment,the number of correct responses of the patients in the iTBS group was higher than that of the 1 Hz rTMS group,and the num-ber of incorrect responses,BSI-CV score suicidal ideation intensity and suicide risk were lower than those of the 1 Hz rTMS group,and the TMT-A time was shorter than that of the 1 Hz rTMS group,with a statistically significant difference between the groups(P＜0.05).During the treatment period,there was no statistically significant difference in the incidence rate of adverse reactions between the two groups(P>0.05).Conclusion:There are significant advantages of iTBS over1 Hz rTMS in treating elderly patients with depression in terms of improving depressive symptoms,reducing suicidal ideation,and improving cognitive performance,and there was no increased treatment safety risk.
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Clinical effect of rigid neuroendoscopy and traditional drilling irrigation and drainage in treating chronic subdural hematoma
Dong Xichao; Wang Fenglu; Zhao Haikang; Rongbo;
2025,40(6):736-739 ,DOI: 10.3969/j.issn.1005-3697.2025.06.012
Abstract:
Objective:To explore the clinical effect of rigid neuroendoscopy and traditional drilling irrigation and drainage in the treatment of chronic subdural hematoma(CSDH).Methods:The clinical data of CSDH patients were retrospectively analyzed.Accord-ing to different treatment methods,they were divided into traditional group(treated with traditional drilling irrigation and drainage)and endoscopic group(treated with rigid neuroendoscopy),48 cases in each group.The differences in perioperative indicators,GCS score,Markwalder grading and NIHSS score were compared between groups,and the complications and recurrence rate in the two groups were counted.Results:The intraoperative blood loss,hospitalization time and immediate postoperative hematoma clearance rate in endoscopic group were better than those in traditional group(P＜0.05),and the total surgical time in traditional group was shorter than that in en-doscopic group(P＜0.05).At 7 days after surgery,the GCS score and Markwalder neurological function grading in the two groups were improved(P＜0.05),but there were no obvious differences between the two groups(P>0.05).Different time points after surgery,the NIHSS score in both groups was reduced compared to before surgery(P＜0.05),and the score was lower in endoscopic group than that in traditional group(P＜0.05).The complications showed no significant differences(P>0.05),and the recurrence rate of hematoma was lower than that in traditional group(P＜0.05).Conclusion:Both rigid neuroendoscopy and traditional drilling irrigation and drainage can effectively treat CSDH,but neuroendoscopy has higher hematoma clearance rate,shorter hospitalization time and lower postoperative recurrence rate,which is worthy of clinical promotion.
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Analysis of prognostic factors in patients with septic cardiomyopathy and development of prediction model
Li Pengfei; Lin Jiafu; Yuan Shun; Jiang Zhi;
2025,40(6):764-769 ,DOI: 10.3969/j.issn.1005-3697.2025.06.018
Abstract:
Objective:To investigate the predictive factors of prognosis in patients with septic cardiomyopathy and building pre-diction model.Methods:A retrospective study was conducted on 156 patients with purulent pericarditis,and they were divided into a model set and a validation set in a 7∶3 ratio.The patients in the model set were divided into survival and death groups according to dif-ferent outcomes.The influence factors on the prognosis of the model set patients were evaluated by multivariate Cox regression analysis,and a ROC curve and decision curve analysis(DCA)were used to validate the model using R software.Results:Multiple COX regres-sion analysis indicated that lactic acid,D-dimer,interleukin-6,APACHE-Ⅱscore and SOFA score were independent risk factors for 28 deaths in hospital with septic cardiomyopathy.ROC curve analysis results showed that the predictive value curve area(AUC)of the model set was 0.90,and the AUC of the validation set was 0.83,indicated that the model has good value in the prognosis of patients with septic cardiomyopathy.The results of the clinical decision curve(DCA curve)suggested that the clinical benefit of the validation set and the model set in this model was superior to the"assume all patients die"(all)and"assume all patients die"(none)curves.Conclusion:The prediction model has a good fit to the actual and correction curves,approaching the ideal curve,and has certain clini-cal practical value for patients with septic cardiomyopathy.
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Curative effect of Zixiandan combined with dipyridamole and its influences on levels of urine immunoglobulin G and β2 microglobulin in children with Henoch-Schonlein purpura
Zhang Lili; Shang Lili; Cui Heqing;
2025,40(5):573-576 ,DOI: 10.3969/j.issn.1005-3697.2025.05.006
Abstract:
Objective:To explore the curative effect of Zixiandan combined with dipyridamole and its influences on levels of u-rine immunoglobulin G and β2 microglobulin in children with Henoch-Schonlein purpura.Methods:According to different treatment methods,80 children with Henoch-Schonlein purpura were divided into control group(n=40,routine comprehensive treatment+dipy-ridamole)and observation group(n=40,Zixiandan on basis of control group),the course of treatment was 7～14 days.Clinical cura-tive effect,scores of TCM syndromes,recovery time of symptoms,renal function and adverse reactions were compared between the two groups.Results:The effective rate of treatment was higher in observation group than in control group(95.00%vs.72.50%,P<0.05).After treatment,scores of TCM syndromes were lower in observation group than control group(P<0.05),disappearance time of lbuminuria,remission time of purpura,length of hospital stay,disappearance time of joint symptoms and remission time of ab-dominal pain were shorter in observation group(P<0.05),levels of urine β2-MG and IgG were lower in observation group(P<0.05).There was no statistically significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion:Zixiandan combined with dipyridamole can improve TCM symptoms,reduce renal injury and promote recovery process in children with Henoch-Schonlein purpura,which has significant curative effect.
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Diagnostic value of multimodal magnetic resonance imaging in grading and Subtype of meningiomas
Ji Xiaoxiao; Lv Hairong; Jia Rongrong; Zhao Feng;
2025,40(6):713-718 ,DOI: 10.3969/j.issn.1005-3697.2025.06.007
Abstract:
Objective:To analyze the diagnostic value of multimodal magnetic resonance imaging(MRI)in grading and Sub-type of meningiomas.Methods:A total of 98 patients with meningiomas were reviewed.They were divided into benign group(n=78)and malignant group(n=20)according to the pathological examination results.All of them underwent conventional MRI scan and mul-timodal MRI examination upon admission.The multimodal MRI parameters[apparent diffusion coefficient(ADC),local cerebral blood flow(rCBF),magnetic sensitive signal intensity(ITSS),N-acetylaspartate(NAA)/choline(Cho),NAA/creatine(Cr),Cho/Cr]of patients with benign and malignant meningiomas,and patients with different subtypes of benign meningiomas were compared.The diag-nostic value of multimodal MRI for meningioma classification and grading were analyzed by receiver operating characteristic(ROC)curves.Results:The ADC,NAA/Cho ratio and NAA/Cr ratio of meningiomas in the malignant group were lower than those in the be-nign group(P＜0.05).The rCBF,ITSS and Cho/Cr ratio were higher than those in the benign group(P＜0.05).The AUC values of three-dimensional arterial spin labeling(3D-ASL),diffusion-weighted imaging(DWI),susceptibility-weighted imaging(SWI)and proton magnetic resonance spectroscopy(1 H-MRS)for grading of meningiomas were 0.723,0.735,0.777 and 0.870.The AUC of com-bined diagnosis was 0.957.There were significant differences in rCBF,ITSS,NAA/Cho ratio,NAA/Cr ratio and Cho/Cr ratio among patients with different subtypes of benign meningiomas(P＜0.05).Conclusion:Multimodal MRI is helpful in clinical grading and Subtype of meningiomas.
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Efficacy of recombinant human interferon α-1b combined with budesonide aerosol inhalation in treating acute bronchitis in children
Wei Qiong; Rao Rui; Xu Wenyi; Yang Luling; Lu Zhili; Li Zhixin;
2025,40(6):774-777 ,DOI: 10.3969/j.issn.1005-3697.2025.06.020
Abstract:
Objective:To explore the clinical efficacy of recombinant human interferon α-1b combined with budesonide and sin-gle budesonide aerosol inhalation in the treatment of acute bronchitis in children.Methods:106 children with acute bronchitis were se-lected as study samples,and were divided into control group and study group by adopting different treatment methods,with 53 cases in each group.The children in the control group were treated with budesonide aerosol inhalation inhalation in addition to conventional treatment,while the children in the study group were combined with budesonide and recombinant human interferon α-1b aerosol inhala-tion inhalation on the basis of conventional treatment,both groups were treated for 1 week.The regression times of clinical symptoms,changes in serum inflammatory factors levels[procalcitonin(PCT),serum C-reactive protein(CRP)],blood routine[white blood cell count(WBC),neutrophil percentage(NEU%)]and lung function indexes[maximum expiratory volume in one second(FEV1),FEV1 to forced vital capacity ratio(FEV1/FVC),and maximum expiratory flow(PEF)]before treatment and after 1 week of treatment and adverse reactions during treatment were compared.Results:The regression times of clinical symptoms in study group were shorter than those in control group(P＜0.05).After 1 week of treatment,the levels of serum PCT,CRP,WBC and NEU%were lower in study group than those in control group(P＜0.05).The FEV1/FVC,PEF and FEV1 in study group were higher compared with those in con-trol group(P＜0.05).During treatment,there were no statistical differences in the incidence rates of adverse reactions between both groups(P>0.05).Conclusion:Recombinant human interferon α-1b combined with budesonide aerosol inhalation inhalation for chil-dren with acute bronchitis can quickly relieve the clinical symptoms,and improve the inflammation level,and it has high safety.
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Influence of placental morphology and pathological changes on neonatal outcome in patients with premature rupture of membranes
Gold mineralization; Zhong Hui; Long Luwen; Zhong Wenna;
2025,40(6):787-790 ,DOI: 10.3969/j.issn.1005-3697.2025.06.023
Abstract:
Objective:To study the influence of placental morphology and pathological changes on neonatal outcome in patients with premature rupture of membranes(PROM).Methods:100 parturients with PROM were selected as the study group,and 100 nor-mal parturients without PROM during the same period were selected as the control group.HE staining was used to observe the Placental pathological changes and monitor pregnancy outcome.The Placental pathological changes and pregnancy outcome were compared be-tween the two groups.The influencing factors of premature rupture of membranes were analyzed by Single factor and multiple factor Lo-gistic regression analysis.Results:The incidence of placental pathological changes in the study group was higher(P＜0.05).The inci-dence of adverse pregnancy outcome in the study group was higher than that in the control group(23.00%vs.5.00%,P＜0.05).Uni-variate analysis showed that there were no statistically significant difference in the incidence of uterine malformations,cervical dysfunc-tion,polyhydramnios,anemia during pregnancy,placenta previa,and history of induced labor or miscarriage between the two groups(P>0.05).In the study group,gestational diabetes,hypertension,abnormal fetal position,multiple births,reproductive tract infection and unknown causes were all higher(P＜0.05).Logistic regression analysis showed that the abnormal fetal position,multiple births,and reproductive tract infection were all independent risk factors for premature rupture of membranes(P＜0.05).Conclusion:Prema-ture rupture of membranes is affected by abnormal fetal position,multiple pregnancies,and reproductive tract infections,and the placenta of patients with premature rupture of membranes has pathological changes,which will lead to poor neonatal outcome.
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Clinical study of Nalbuphine as local anesthetic adjuvant for nerve block analgesia in elderly patients after total hip arthroplasty
Wang Huajuan; Liu Qiyu; Liu Jing; Li Mingming; Song Man; Li Fengjuan; Zheng Xiaozhen;
2025,40(10):1253-1258 ,DOI: 10.3969/j.issn.1005-3697.2025.10.007
Abstract:
Objective:To observe the effect of ultrasound-guided suprainguinal fascia iliaca compartment block(U-SFICB)with ropivacaine combined with different doses of nalbuphine on analgesia in elderly patients after total hip arthroplasty(THA).Methods:101 elderly patients with unilateral THA under general anesthesia were divided into four groups according to different anesthesia regimens:100 mg ropivacaine+normal saline group(R group,n=26),100 mg ropivacaine+10 mg nal-buphine group(LN group,n=25),and 100 mg ropivacaine+15 mg nalbuphine group(MN group,n=25),and 100 mg ropiv-acaine+20mg nalbuphine group(HN group,n=25),all were diluted to 30 mL with 0.9%sodium chloride injection.All four groups underwent U-SFICB in the post-anesthesia care unit(PACU),and sufentanil was used for patient-controlled intravenous analgesia after surgery.Comparison between groups of postoperative 4,8,12,24,and 48 h of resting and motion state of Numer-ical rating scale(NRS).Each group received 12 h preoperatively,12 and 24 h postoperatively,Interleukin 6(IL-6),Tumor nec-rosis factor-α(TNF-α),Interleukin 10(IL-10)concentration,the first start time of postoperative analgesia pump and 48 h of sufentanil use in each group,the early postoperative recovery of each group,and the occurrence of analgesia related adverse reac-tions 48 h after surgery were compared between groups.Results:Compared with R group and LN group,NRS score in MN and HN group was decreased at all time points(P<0.05),IL-6 and TNF-α concentrations were decreased at all time points(P<0.05),IL-10 concentrations were increased at all time points(P<0.05),and the start-up time of postoperative analgesia pump was significantly extended(P<0.05).The amount of sufentanil was significantly decreased 48 hours after operation(P<0.05).Compared with R group,LN group and HN group,the time of first anal exhaust and getting out of bed in MN group were significantly shortened(P<0.05).The incidence of adverse reactions was significantly decreased 48 h after operation(P<0.05).Compared with HN group,the start-up time of postoperative analgesic pump in MN group was significantly short-ened(P<0.05),and the overall incidence of adverse reactions was the lowest at 48 h after surgery(P<0.05).Conclusion:15 mg nalbuphine combined with ropivacaine can significantly prolong the duration of U-SFICB analgesia after THA in elderly pa-tients,and the first postoperative anal exhaust time and the first time of getting out of bed activity are the shortest,and the inci-dence of adverse reactions is the lowest within 48 hours after surgery,which can be clinically promoted and applied.
Basic Medical Research
Clinical medical research
Basic Medical Research
Clinical medical research
Basic Medical Research
Clinical medical research
Editor in chief：Dai Rongyang
Deputy Editor-in-Chief：Li Zhi，Zhang Xiaoming
Organizer：Sichuan Provincial Department of Education
GoverningBody：North Sichuan Medical College
Phone：0817-2242637
Email：xuebaocby@126.com
ISSN1005-3697
CN51-1254/R
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