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Effect of RNA binding protein ZCCHC17 on the proliferation,migration,and invasion capacity of hepatocellular carcinoma
Liang Jiadong; Liu Jinglin; Huang Yunmei; Yang Weipeng; Luo Chunying;
2025,40(1):1-5 ,DOI: 10.3969/j.issn.1005-3697.2025.01.001
Abstract:
Objective:To explore the effect of RNA binding ZCCHC17 on proliferation,migration and invasion ability of hepato-cellular carcinoma(HCC).Methods:RT-qPCR and Western blot were used to detect the expression levels of the cell lines ZCCHC17 selected.Then,the proliferation,migration,and invasion ability of cell lines were examined by clonformation assay,CCK-8 assay,scratch assay and Transwell invasion assay.Results:ZCCHC 17 was lowly expressed in human normal liver cell line L02(P＞0.05),high ex-pression was observed in the Bel7404 hepatocellular carcinoma cell line(P＜0.05),and low expression was observed in the MH-CC97H hepatocellular carcinoma cell line was diminished after ZCCHC17 knockdown(P＞0.05).The results of clone formation assay and CCK-8 showed that the proliferation of Bel7404 cell lines was diminished after ZCCHC17 knockdown(P＜0.05),the scratch test results showed that the migration ability of Bel7404 cell lines decreased(P＜0.05),the Transwell experiment showed that the invasion ability of Bel7404 cell lines was weakened(P＜0.05).Conclusion:High expression of ZCCHC17 can promote the proliferation,migra-tion and invasion of hepatocellular carcinoma.
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Palmitoylation of m5C reader YBX1 by ZDHHC20 promotes colorectal cancer progression through regulating mRNA m5C methylation
Luocheng; Meng Changyuan; Xia Shusen; Huang Shan; Peng Hong;
2025,40(1):6-12 ,DOI: 10.3969/j.issn.1005-3697.2025.01.002
Abstract:
Objective:To explore the molecular mechanism that palmitoyl acyltransferase 20(ZDHHC20)participates in the pathogenesis of colorectal cancer(CRC)through Palmitoylation modification to regulate 5-methylcytosine(m5C)reader Y-box binding proteinl(YBX1)protein stability,and improve mRNA m5C modification level.Methods:The expression of ZDHHC20 mRNA and pro-tein in CRC was analyzed by bioinformatics.Further analysis of the association among ZDHHC20 expression levels and the clinicopatho-logical features of CRC patients was performed.The expression level of ZDHHC20 in CRC cells was verified using RT-qPCR.A CCK-8 assay was used to detect the effect of ZDHHC20 on the proliferation of CRC cells.Transwell was used for cell invasion assays.The rela-tionship between the expression of ZDHHC20 and YBX1 in CRC and the palmitoylation site of YBX1 was investigated by bioinformatics database analysis.Palmitoylation protein was enriched by ABE method and Westernblot experiment was used to detect YBX1 palmitoyla-tion modification in CRC.Overexpression of ZDHHC20 was used to detect the palmitoylation modification level of YBX1.The Western blot was used to measure the stability of YBX1 protein under 2-BP and actinomycin.The influence of YBX1 on the proliferation and mi-gration of CRC cells was measured by CCK-8 and Transwell.LC-MS was used to analyze the impact of YBX1 on m5C modification level in CRC cells.Results:An upregulation of ZDHHC20 mRNA and protein were observed in the sample CRC cells and tissues(P＜0.05).ZDHHC20 expression was correlated with age,tumor stage and lymph node metastasis(P＜0.05).Overexpression of ZDHHC20 contributes to proliferation and invasion in CRC cells(P＜0.05).The expression between ZDHHC20 and YBX1 were posi-tively correlated(P＜0.05),and YBX1 had multiple palmitoylation modification site.YBX1 was a palmitoylated protein and ZDHHC20 could increase YBX1 palmitoylation modification level and improve the stability of YBX1 protein(P＜0.05).YBX1 could enhance CRC cell proliferation and invasion.Also YBX1 could improve m5C modification level in CRC(P＜0.05).Conclusion:ZDHHC20 can improve the stability of YBX1 protein through Palmitoylation modification and mRNA m5C modification level,and enhance CRC cell proliferation and invasion.
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Mechanism of protective effect of reduced glutathione on acute liver injury induced by acetaminophen
Chen Li; Wu Silin; Zhang Chengda; He Jun; Wang Jisheng; Pang Jun;
2025,40(1):13-20 ,DOI: 10.3969/j.issn.1005-3697.2025.01.003
Abstract:
Objective:To explore the molecular mechanisms of protective effects of glutathione(GSH)in mice with acetamino-phen-induced acute liver injury.Methods:120 mice were randomly divided into 3 groups:Control,APAP,GSH,with 40 in each group.After establishing the model,mice in the GSH groups were intraperitoneally injected with GSH(0.40 g/kg)for 3 days.Subsequently,liver tissue samples were collected.SWATH combined with liquid chromatography-tandem mass spectrometry was used to analyze the protein expression profile,and the differentially expressed proteins between APAP group and Control group,GSH group and APAP group were screened out.GO functional annotation and KEGG pathway enrichment analysis were performed for the above differential proteins.Protein interaction network(PPI)was constructed by String database and Cytoscape software,and the key proteins were verified by im-munohistochemical staining.Results:A total of 310 differentially expressed proteins were screened out between APAP group and Control group.A total of 172 differentially expressed proteins were screened out between the GSH group and the APAP group.The enrichment a-nalysis of the above differentially expressed proteins showed that the differential proteins were mainly enriched in the functions and pathways of nitrogen metabolism,fatty acid metabolism,and organic acid metabolism.Further analysis identified 17 differentially ex-pressed proteins between APAP group vs.Control group and GSH group vs.APAP group.Anxa5,Rgn,Tagln,Vim and Usp5 may be re-lated to apoptosis.Anxa5 and Rgn protein expression was higher in the APAP group than in the Control group(P＜0.05).In contrast,Anxa5 protein expression decreased and Rgn protein expression increased in the GSH group compared to the APAP group(P＜0.05),there was no statistically significant difference in Tagln,Vim,and Usp5(P＞0.05).Conclusion:GSH protects mice from APAP-in-duced liver injury by inhibiting cell apoptosis.Anxa5,Rgn,Tagln,Vim,and Usp5 are potential targets.
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Effect of auricular point sticking combined with foot bath in promoting postoperative recovery of patients with adenomyopathy and the mechanism of analgesia and anti-anxiety
Liu Juanping; Zeng Weiwei; Zhu Xiaozhen;
2025,40(1):21-24 36 ,DOI: 10.3969/j.issn.1005-3697.2025.01.004
Abstract:
Objective:To explore the effect of auricular point sticking combined with foot bath in promoting the postoperative re-covery of patients with adenomyopathy and the mechanism of analgesia and anti-anxiety.Methods:A total of 107 patients with adenomy-opathy who underwent high intensity focused ultrasound(HIFU)were divided into observation group(n=54)and control group(n=53)according to different intervention methods.Patients in the control group were given foot bath intervention after surgery.Observation group was given auricular point sticking intervention on the basis of control group after operation,and both groups were continuously in-tervened for 5 days.The VAS score,TCM syndrome score,mental state[Hospital Anxiety and Depression Scale(HADS)],number of sleep and wake times,serum neurotransmitters[β-endorphin(β-EP),5-hydroxytryptophan(5-HT)]and inflammatory factors[Inter-leukin-6(IL-6),soluble vascular cell adhesion molecule-1(sVCAM-1)],adverse reactions,and recurrence rate within 1 year were compared between the two groups.Results:At 1,3 and 5 d after surgery,the VAS scores in both groups decreased gradually,and the ob-servation group was lower than the control group(P＜0.05).After 5 days of treatment,TCM syndrome score and anxiety HADS(A)score were lower in both groups than those of 1 day after surgery(P＜0.05).The observation group was lower than the control group(P＜0.05).The number of sleep and wake times within 5 days after surgery in the observation group was lower than that in the control group(P＜0.005).After 5 days of treatment,the serum levels of β-EP and 5-HT in the two groups were higher than 1 day after surgery(P＜0.05),and the observation group was higher than the control group(P＜0.05).The levels of IL-6 and sVCAM-1 were lower than 1 day after surgery(P＜0.05),and the observation group was lower than the control group(P＜0.05).After 1 year of follow-up,there was no statistically significant difference in the recurrence rate between the two groups(P＞0.05).Conclusion:Auricular point stick-ing combined with foot bath can effectively promote the postoperative recovery of patients with adenomyopathy,reduce postoperative pain,improve clinical symptoms and anxiety,which may be related to the analgesic and anti-anxiety effects by regulating neurotransmit-ters and reducing the level of inflammatory factors,with good safety and not easy to relapse.
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Influence of sintilimab combined with platinum-based chemotherapy on immune indexes and survival cycle in patients with advanced gastric cancer
Zhao Xiaomin; Ding Jie; Wang Juan;
2025,40(1):25-28 ,DOI: 10.3969/j.issn.1005-3697.2025.01.005
Abstract:
Objective:To explore the influence of combination of sintilimab and platinum-based chemotherapy on immune inde-xes and survival cycle in patients with advanced gastric cancer(AGC).Methods:82 patients with AGC were included and divided into chemotherapy group(n=41,platinum therapy)and combined group(n=41,sintilimab+platinum therapy)according to different treatment regimens.21 days was one cycle,both groups were treated for 3 cycles,followed up for 1 year.The immune indicators[CD3+T cell,CD4+T cell,CD8+T cell and CD4+/CD8+T cell levels]and angiogenesis indexes[endostatin(ES),angiopoietin-2(Ang-2),vascular endothelial growth factor(VEGF)]before and after treatment,clinical efficacy and incidence rates of adverse reactions were compared between the two groups.Kaplan Merier was used to analyze the 1-year survival status of the two groups.Results:After treatment,compared with the chemotherapy group,the levels of CD3+,CD4+,CD4+/CD8+,ES,total effective rate and 1-year survival rate in the combined group were higher(P＜0.05)while the levels of CD8+,Ang-2 and VEGF were lower(P＜0.05).There were no statistical differences in adverse reactions between groups(P＞0.05).Conclusion:The application of sintilimab combined with plati-num-based chemotherapy for patients with AGC can reduce the influence of chemotherapy on immune function,inhibit the tumor angio-genesis,and enhance the clinical efficacy and survival rate,and it has safety.
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Clinical effect of botulinum toxin type A combined with CO2 fractional la-ser with different parameters on improving periorbital skin aging
Han Dongmei; Ma Ying; Liu Xiaohua; Zhao Lan;
2025,40(1):29-32 ,DOI: 10.3969/j.issn.1005-3697.2025.01.006
Abstract:
Objective:To explore the clinical effect of botulinum toxin type A combined with different parameters of CO2 frac-tional laser with different parameters on improving periorbital skin aging.Methods:A total of 108 patients with periorbital skin aging were enrolled as the research subjects.Both groups were given botulinum toxin type A combined with CO2 fractional laser.54 patients with parameters of 50 mJ and 8％were collected as group A,and 54 patients with parameters of 30 mJ and 4％were included in group B.The Global Aesthetic Improvement Scale(GAIS),skin images scores,skin barrier function,pain level[Visual Analogue Scale(VAS)]and incidence rates of complications were compared between groups.Results:At 6 months after treatment,the GAIS grading in group A was better than that in group B(P＜0.05).The skin images scores and transcutaneous water loss in both groups were lower than those before treatment(P＜0.05),and the indicators in group A were lower than those in group B(P＜0.05).The skin grease content and water content of stratum corneum were increased in both groups(P＜0.05),and the indicators in group A were higher than those in group B(P＜0.05).Immediately after treatment,the VAS score was higher in group A than that in group B(P＜0.05).There were no statistical differences in the incidence rates of complications between groups(P＞0.05).Conclusion:Botulinum toxin type A combined with 50 mJ,8％CO2 fractional laser can improve the overall cosmetic effect of patients,but the immediate postoperative pain level is higher.
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Analysis of the relationship between ABCB1 gene polymorphism and the therapeutic effect of levetiracetam in children with epilepsy
Wang Zuohua; Duan Wenhao; Wang Chunxia; Nie Wensha; Zheng Yuxia; Zhang Xia; Wang Huiping;
2025,40(1):33-36 ,DOI: 10.3969/j.issn.1005-3697.2025.01.007
Abstract:
Objective:To investigate the correlation between ATP-binding cassette B subfamily 1 transporter(ABCB1)gene polymorphism and the efficacy of levetiracetam(LEV)in children with epilepsy.Methods;A total of 94 children with epilepsy were se-lected as the research subjects.According to the curative effect,they were divided into effective group(n=76)and ineffective group(n=18).Polymerase chain reaction and fluorescence in situ hybridization(PCR-FISH)were used to detect ABCBI genotypes in all children,and clinical data were collected.The clinical data and ABCBI genotype were compared between the two groups.Logistic regres-sion model was used to determine the risk factors of LEV treatment failure.Results;Compared with the effective group,the proportion of first onset as myoclonic type,seizure frequency,EEG abnormality and birth suffocation in the ineffective group were higher(P＜0.05).Compared with the effective group,the proportion of ABCB1 C3435 T TT type in the ineffective group was higher,and the frequency of T gene distribution was higher,the differences were statistically significant(P＜0.05).Logistic regression analysis showed that seizure frequency(OR=5.812),birth asphyxia(OR=3.274),and ABCB1 genotype(OR=7.675)were independent influencing factors of LEV efficacy in children with epilepsy(P＜0.05).The risk of treatment failure with TT genotype was higher than that with CC and CT genotypes.Conclusion:ABCB1 gene polymorphism is closely related to the efficacy of epilepsy in children.TT genotype may be a risk factor for LEV treatment failure.In addition,the risk of ineffective LEV treatment is higher in patients with higher seizure frequency and birth asphyxia.
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Comparison of short-term and long-term curative effects of thoracoscopic-assisted transthoracic internal fixation and traditional thoracotomy on pa-tients with multiple rib fractures
Fu Jingliang; Du Xiangkun; He Rongqi;
2025,40(1):37-40 ,DOI: 10.3969/j.issn.1005-3697.2025.01.008
Abstract:
Objective:To compare the short-term and long-term curative effects of video-assisted thoracoscopic surgery(VATS)transthoracic internal fixation and traditional thoracotomy on patients with multiple rib fractures.Methods:The data of 106 patients with multiple rib fractures were retrospectively analyzed.The patients were classified into two groups by using to the different surgical meth-ods,including traditional group(traditional thoracotomy)and VATS group(VATS transthoracic internal fixation).The propensity score matching method(caliper=0.01,at a ratio of 1:1)was used to match the two groups.53 cases were finally obtained in each group.The perioperative indicators,incidence rates of complications,postoperative pain degree(VAS),disease recovery status and long-term curative effect were compared between groups.Results:The VATS group had shorter surgical time and total hospitalization time(P＜0.05),and the incidence rates of complications were lower(P＜0.05).At 5 and 7 days after surgery,the VAS score was de-creased with time(P＜0.05),and the VAS score in VATS group at 1,5,and 7 days after surgery was lower(P＜0.05).The fracture alignment rate,incision healing rate and excellent and good rate of postoperative effect were higher in VATS group(P＜0.05).Conclu-sion:Compared with traditional thoracotomy,VATS transthoracic internal fixation can more accurately locate the fracture,shorten the surgical time,promote the postoperative recovery of multiple rib fractures,with few postoperative complications and ideal long-term cura-tive effect,which is worthy of clinical application.
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Efficacy of holmium laser enucleation of the prostate on benign prostatic hyperplasia and its influence on serum prostate specific antigen and erec-tile function of patients
Yu Hongbo; Guan Han; Li Cong; Wang Penghui; Jiang Yao; Xu Pengcheng;
2025,40(1):41-44 ,DOI: 10.3969/j.issn.1005-3697.2025.01.009
Abstract:
Objective:To explore the efficacy of holmium laser enucleation of the prostate(HoLEP)in the treatment of benign prostatic hyperplasia(BPH)and the influence on serum prostate specific antigen(PSA)and erectile function of patients.Methods:A total of 98 BPH patients were selected as the study subjects,and they were divided into HoLEP group and TURP group according to dif-ferent surgical treatment methods,with 49 cases in each group.HoLEP group were treated with HoLEP,and TURP group were treated with TURP.The perioperative related indicators(surgical time,bleeding volume,catheterization time,bladder flushing time,and hospital stay),clinical efficacy,serum PSA level,erectile function[International Index of Erectile Function(IIEF-5)],urodynamic parameters[maximum urinary flow rate(Qmax),post voiding residual urine volume(PVR)]and incidence rates of complications were compared between the two groups.Results:The surgical time,bleeding volume,catheterization time,bladder flushing time,and hospital stay in Ho-LEP group were shorter or less than those in TURP group(P＜0.05).At 6 months after surgery,the total clinical efficacy was higher in HoLEP group than that in TURP group(P＜0.05).The PSA level and PVR in the two groups were decreased(P＜0.05),and the in-dicators in HoLEP group were lower than those in TURP group(P＜0.05).The IIEF-5 scores were declined in both groups(P＜0.05),and the HoLEP group had higher scores than the TURP group(P＜0.05).The Qmax in both groups was risen(P＜0.05),and the HoLEP group had higher Qmax(P＜0.05).The incidence rates of complications in HoLEP group were lower compared to TURP group(P＜0.05).Conclusion:HoLEP can enhance the clinical efficacy of patients with BPH,and can protect e-rectile function,and reduce PSA level and incidence rates of complications.
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Difference of the effect of hysteroscopic uterine adhesion incision and re-section on endometrial receptivity
Luo Qiao; Feng Tiantian; Qiu Xia; He Deng; Lan Li; Zeng Yuhua;
2025,40(1):45-48 53 ,DOI: 10.3969/j.issn.1005-3697.2025.01.010
Abstract:
Objective:To investigate the effect of hysteroscopic incision and excision of uterine adhesion on endometrial receptiv-ity.Methods:60 patients with moderate and severe uterine adhesions were divided into the study group and the control group according to the different treatment methods,30 cases in each group.Patients in the study group were treated we used a plasma electrode needle under hysteroscopy to just strip away adhesion.Whereas,the control group additionally required the use of an electrocution ring removal for scar excision.Endometrial receptivity indexes were evaluated by transvaginal ultrasound.Follow-up pregnancy outcome.Results:En-dometrial vascular index(VI)and vascular blood flow index(VFI)were higher in the study group than in the control group(P＜0.05).There was no statistically significant difference between the two group in intraoperative situation,post-operative hystero-scopic evaluation,menstrual recovery,endometrial type,blood flow pattern,endometrial thickness(ED),endometrial volume(EV)and blood flow index(FI)(P＞0.05),and no statistically dignificant difference in pregnancy outcome(P＞0.05).Conclusion:Under hysteroscopy,simple plasma electrode needle incision and electrocution ring removal could effectively restore uterine cavity morphology.The former is simpler than the latter,and has less influence on the blood flow parameters in endometrial receptivity.
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Efficacy and safety evaluation of calcium,nicergoline combined with fluna-rizine in the treatment of peripheral vestibular vertigo
Zheng Jie; Li Fulai; Feng Chenfang; Zhang Nan; Liu Ying; Wang Zhanqiang; Yang Bin; Wang Huan;
2025,40(1):49-53 ,DOI: 10.3969/j.issn.1005-3697.2025.01.011
Abstract:
Objective:To investigate the efficacy and safety of calcium,niergoline combined with flunarizine in the treatment of peripheral vestibular vertigo.Methods:A total of 106 patients with peripheral vestibular vertigo were selected.They were divided into group A(Flunarizine,n=36),group B(Nyergoline+Flunarizine,n=35)and group C(calcium+Nyergoline+Flunarizine,n=35)according to different intervention methods,all groups were treated for 6 weeks.The degree of dizziness[dizziness handicap inven-tory(DHI)],dizziness symptoms[vertigo symptom scale-short form(VSS-SF),vestibular symptom index(VSI)scores],hemodynam-ic parameters[whole blood viscosity,hematocrit,vertebral artery mean velocity(Vm),basilar artery Vm],treatment effect,adverse re-actions during treatment and recurrence 6 months after treatment were compared among the groups before and after treatment.Results:After treatment,DHI scores,VSS-SF scores and VSI scores of physical,functional and emotional dimensions in group B and group C were lower than those in group A(P＜0.05).After treatment,the clinical effective rate of group B and group C was higher than that of group A(P＜0.05).After treatment,the whole blood viscosity and hematocrit of groups B and C were lower than those of group A(P＜0.05),and the Vm of vertebral artery and basilar artery were higher than those of group A(P＜0.05).There was no significant difference in the incidence of adverse reactions among the three groups(P＞0.05).The recurrence rate of the three groups 6 months after treatment was significantly different(27.78％vs.25.71％vs.5.71％,P＜0.05).Conclusion:Calcium,niergoline combined with flunarizine is effective in the treatment of peripheral vestibular vertigo,and is beneficial to reduce the risk of disease recurrence.
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Analysis of influencing factors of anemia in premature infants
Li Guoming; Fang Ping; Zhao Jing; Jiang Lin;
2025,40(1):54-57 ,DOI: 10.3969/j.issn.1005-3697.2025.01.012
Abstract:
Objective:To explore the related factors of anemia in premature infants.Methods:410 premature infants were retro-spectively collected and divided into anemia group(n=153)and non-anemia group(n=257)based on whether anemia occurs or not.The general information such as collect sex,number of fetuses,conception,mode of delivery,premature rupture of membranes,maternal anemia,maternal cholestasis,gestational hypertension,gestational diabetes,gestational hypothyroidism,prenatal use of hormones,intrau-terine distress,cardiopulmonary resuscitation,preeclampsia,pulmonary surfactant use,mechanical ventilation,intravenous nutrition,neo-natal septicemia,neonatal respiratory distress syndrome,gestational age,birth weight,length of stay,birth hemoglobin content,birth red blood cell count,mechanical ventilation,pregnancy,number of births,maternal age,multivariate,pregnancy,number of births,and mater-nal age were compared between two groups.Logistic regression analysis was used to screen the independent influencing factors.Results:There were statistically significant difference in intrauterine distress,cardiopulmonary resuscitation,preeclampsia,neonatal sepsis,neo-natal respiratory distress syndrome,mechanical ventilation,pulmonary surfactant use,intravenous nutrition use,length of hospital stay,gestational age,mechanical ventilation time,birth hemoglobin content,birth weight,and birth red blood cell count between two groups(P＜0.05).There was no statistically significant difference in other data(P＞0.05).The results of Logistic regression analysis showed that the number of days in hospital,the use of parenteral nutrition and preeclampsia were the risk factors of anemia in premature infants(OR=1.105,2.424,5.113,P＜0.05),birth weight and hemoglobin content were protective factors for anemia in premature infants(OR=0.999,0.904,P＜0.05).Conclusion:Lower birth weight,lower birth hemoglobin content,longer hospital stay,pregnant women with preeclampsia and use of intravenous nutrition will increase the risk of anemia in premature infants.
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The efficacy and safety of unilateral biportal endoscopic in the treatment of elderly patients with lumbar disc herniation and degenerative scoliosis
Wang Juan; Yu Jingjing; Hu Chenchen;
2025,40(1):58-61 ,DOI: 10.3969/j.issn.1005-3697.2025.01.013
Abstract:
Objective:To investigate the efficacy and safety of unilateral biportal endoscopic(UBE)in the treatment of elderly patients with lumbar disc herniation(LDH)and degenerative scoliosis.Methods:90 elderly patients with LDH complicated with degen-erative scoliosis were selected,and they were divided into UBE group(n=45)and open group(n=45)according to different inter-vention methods.The indexes related to surgery and hospitalization,the Japanese Orthopaedic Association(JOA)lumbar function score,Oswestry Disability Index(ODI),intervertebral height,spinal canal area,lumbar scoliosis Cobb Angle,and visual analog score(VAS)were compared between the two groups.The incidence of complications during hospitalization was recorded and compared be-tween the two groups.Results:The intraoperative blood loss in UBE group was lower than that in open group(P＜0.05),and the first time of getting out of bed and the length of hospital stay were shorter than that in open group(P＜0.05).The operative time of UBE group was longer than that of open group(P＜0.05).3 months after surgery,there were no significant differences in JOA lumbar func-tion score,ODI,intervertebral height,vertebral canal area and Cobb Angle between the two groups(P＞0.05).The VAS scores in the UBE group were lower than those in the open group at 1 day and 3 months after surgery(P＜0.05).During hospitalization,the compli-cation rate in the UBE group was lower than that in the open group(P＜0.05).Conclusion:The efficacy of UBE in the treatment of elderly LDH complicated with degenerative scoliosis is comparable to that of traditional open surgery,but UBE is beneficial to reduce in-traoperative blood loss,relieve pain,promote postoperative recovery of patients,and reduce the risk of postoperative complications,but the operation time is relatively prolonged.
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Efficacy of Augmented Lou Bei Er Chen Decoction in pulmonary sarcoido-sis of Qi stagnation and phlegm stasis with anxiety and depression:an ob-servational study based on state-target syndrome differentiation
Zhao Rong; Tian Xiaoya; Niu Caiqin;
2025,40(1):62-67 ,DOI: 10.3969/j.issn.1005-3697.2025.01.014
Abstract:
Objective:To observe the efficacy of Augmented Lou Bei Er Chen Decoction in treating pulmonary nodule(PN)characterized by Qi stagnation and phlegm stasis with concomitant anxiety and depression based on the theory of"state-target syndrome differentiation".Methods:A retrospective analysis was conducted on the clinical data of 80 patients diagnosed with PN presenting Qi stagnation and phlegm stasis with anxiety and depression.These patients were divided into two groups:The control group,consisting of 40 participants,received Shu Gan Jie Yu Capsules.On the other hand,the treatment group,with also 40 participants,was administered Augmented Lou BeiEr Chen Decoction in addition to Shu Gan Jie Yu Capsules,the course of treatment was 3 months.Clinical efficacy,Traditional Chinese Medicine(TCM)syndrome scores,psychological states[assessed using the Self-rating Anxiety Scale(SAS)and Self-rating Depression Scale(SDS)],and adverse reactions were compared between the two groups at baseline and after 3 months of treatment.Results:After a 3-month treatment period,the treatment group exhibited a significant reduction in the maximum diameter of nodules compared to the control group(P＜0.05).Moreover,there was a notable discrepancy in clinical efficacy between the two groups post-treatment,with the treatment group demonstrating a higher overall clinical effective rate than the control group(47.50％vs.17.5％,P＜0.05).Following the 3-month treatment duration,both groups experienced decreases in SAS,SDS and TCM syndrome scores,these scores were consistently lower in the treatment group compared to those of the control group(P＜0.05).Importantly,sig-nificant differences were observed in TCM syndrome efficacy between the two groups after treatment,with a higher total effective rate of TCM syndrome efficacy found within the treatment group compared to that of the control group(P＜0.05).Notably,no statistically sig-nificant difference was detected regarding adverse reaction incidence between both groups(P＞0.05).Conclusion:Based on the theory of"state-target syndrome differentiation",Augmented Lou Bei Er Chen Decoction intervention in the treatment of PN with anxiety and depression demonstrates significant clinical efficacy and warrants further promotion.
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Risk prediction model of blood glucose fluctuation and complications in di-abetic patients
Huang Xiaoyan; Zang Xunxiong; Wu Haina; Zhao Dandan; Huang Ze; Chen Xiao; Zhi Xiaoyang; Chen Yuna;
2025,40(1):68-72 ,DOI: 10.3969/j.issn.1005-3697.2025.01.015
Abstract:
Objective:To explore the risk factors of blood glucose fluctuation and complications in patients with diabetes.Meth-ods:23 8 patients with type 2 diabetes were retrospectively selected for standardized diagnosis,treatment and follow-up.According to whether the diabetic patients had blood glucose fluctuation and diabetic complications,they were divided into blood glucose fluctuation group(n=54),non-blood glucose fluctuation group(n=184),and diabetic complication group(n=35),non-diabetic complication group(n=203).After 12-month follow-up,the risk factors of blood glucose fluctuation and complications in patients with diabetes were identified by univariate analysis and multivariate regression analysis.And establish a risk prediction model for the occurrence of blood glucose fluctuation and diabetes in patients with diabetes.Results:Univariate and multivariate regression analysis showed that male,smoking,course of disease,number of treatment lines,and higher levels of blood triglycerides(TG)were independent risk factors for causing blood glucose fluctuations in patients(P＜0.05).Age,male,smoking,course of disease,number of treatment lines,glycosylat-ed hemoglobin,higher TG and lower level of glomerular filtration rate(eGFR)were independent risk factors for diabetic complications(P＜0.05).Based on the risk factors,the risk prediction model of blood glucose fluctuation and diabetic complications in patients with diabetes were established.Through internal verification,the area under the curve calculated by ROC curve was 0.954 and 0.934,which proved that the prediction efficiency of the model was better.Conclusion:Male,smoking history,course of disease,number of treatment lines and TG level are independent risk factors for blood glucose fluctuation in patients.Age,male,smoking,course of disease,number of treatment lines,glycosylated hemoglobin,higher TG and lower eGFR levels are independent risk factors for diabetic complications.Based on the risk factors,the risk prediction model of blood glucose fluctuation and complications in patients with diabetes can be estab-lished,and the prediction efficiency of the model is good.
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Correlation between serum Hcy,SAA,LDL/HDL and types of carotid plaques,prognosis in patients with acute cerebral infarction
Zhang Wenbin; Shi Xiufang; Zhou Wenjing;
2025,40(1):73-76 ,DOI: 10.3969/j.issn.1005-3697.2025.01.016
Abstract:
Objective:To analyze and explore the correlation between serum homocysteine(Hcy),serum amyloid A(SAA),low/high-density lipoprotein cholesterol(LDL//HDL)and carotid plaques,prognosis in patients with acute cerebral infarction.Meth-ods:According to stability of carotid plaques,102 patients with acute cerebral infarction were divided into stable group(n=64)and unstable group(n=38).The peripheral blood indicators(Hcy,SAA and LDL/HDL),and carotid plaque stability(vulnerability scores)in the two groups were compared,and their correlation was analyzed.According to prognosis,patients were divided into good prognosis group(n=57)and poor prognosis group(n=45).The peripheral blood indicators(Hcy,SAA and LDL/HDL)were com-pared between the two groups,and receiver operating characteristic(ROC)curve was used to analyze the predictive value of peripheral blood indicators on patient prognosis.Results:Hcy,SAA,LDL/HDL levels and vulnerability scores in stable group were lower than those in unstable group(P＜0.05).Spearman correlation analysis showed that the stability of carotid plaques was positively correlated with Hcy,SAA and LDL/HDL levels(P＜0.05).The levels of Hcy,SAA and LDL/HDL in good prognosis group were lower than those in poor prognosis group(P＜0.05).ROC curves analysis showed that area under the curve(AUC)values of Hcy,SAA and LDL/HDL for predicting prognosis were 0.788,0.796 and 0.676,respectively(P＜0.05).Conclusion:The levels of Hcy,SAA and LDL/HDL are correlated with the stability of carotid plaques in patients with acute cerebral infarction,which also have predictive value in prognosis.
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Efficacy of dapagliflozin combined with rhBNP in the treatment of acute heart failure and its effects on serum related factors
Bao Xianli; Gu Quan; Zhang Chunfang;
2025,40(1):77-80 ,DOI: 10.3969/j.issn.1005-3697.2025.01.017
Abstract:
Objective:To investigate the effect of dapagliflozin combined with recombinant human brain natriuretic peptide(rh-BNP)in patients with acute heart failure(AHF)and its effects on serum related factors.Methods:104 patients with AHF were divided into control group and observation group according to different treatment methods,52 cases in each group.The control group was treated with rhBNP,and the observation group was treated with dapagliflozin combined with rhBNP.The curative effect was evaluated after 7 days of treatment.The left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDD),Kansas Cardiomyop-athy Questionnaire(KCCQ)score,serum N-terminal pro-brain natriuretic peptide(NT-proBNP),cardiac troponin(cTnT),high-sensi-tivity C-reactive protein(hs-CRP)and interleukin-6(IL-6)levels were compared between the two groups.The hospitalization time,in-hospital mortality,30-day readmission rate and adverse reactions of the two groups were statistically analyzed.Results:After treatment,the total effective rate of treatment of the observation group was higher than that of the control group(P＜0.05).Compared with the control group,the observation group had higher LVEF level,KCCQ score and lower LVEDD(P＜0.05),and the serum levels of NT-proBNP,cTnT,hs-CRP and IL-6 in the observation group were lower(P＜0.05).Compared with the control group,the hospitalization time of the observation group was shorter(P＜0.05),there was no significant difference in the incidence of in-hospital death,30-day readmission rate and adverse reactions between the two groups(P＞0.05).Conclusion:Dapagliflozin combined with rhBNP in patients with AHF can reduce inflammation,down-regulate serum NT-proBNP and cTnT levels,improve cardiac function,improve efficacy,and have good safety.
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Application of MRI diffusion weighted imaging and dynamic contrast-en-hanced MRI in diagnosing prostate cancer
Yan Xianjie; Wang Hai; Zhu Wenmiao;
2025,40(1):81-84 ,DOI: 10.3969/j.issn.1005-3697.2025.01.018
Abstract:
Objective:To analyze the application of magnetic resonance imaging(MRI)diffusion weighted imaging(DWI)and dynamic contrast enhanced MRI(DCE-MRI)in the diagnosis of prostate cancer(PCA).Methods:105 patients with suspected PCA were selected as the research subjects,and they underwent DWI and DCE-MRI scanning.With pathological biopsy as the gold standard,Kappa consistency test was used to analyze the accuracy,sensitivity and specificity of DWI,DCE-MRI alone and their combined exami-nation in the diagnosis of PCA.Results:66 cases of PCA and 39 cases of benign prostatic lesions were detected by DWI respectively.The Kappa value of DWI and pathological biopsy in diagnosing PCA was 0.918.DCE-MRI detected 60 cases of PCA and 45 cases of benign prostatic lesions,and the Kappa value of PCA diagnosed by DCE-MRI and pathological biopsy was 0.761.The sensitivity and ac-curacy of DWI for PCA diagnosis were 95.59％and 96.19％,which were higher than 85.29％and 88.57％of DCE-MRI(x2=4.168,4.330,P=0.041,0.037).The Kappa value of DWI+DCE-MRI and pathological biopsy for PCA diagnosis was 0.937,and the sensitivity and accuracy were 98.53％and 97.14％,respectively.Conclusion:DWI has higher accuracy and sensitivity in the diagnosis of PCA than DCE-MRI,and the combined diagnosis of the two can up-regulate the diagnostic efficiency of prostate cancer to a certain extent.
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Therapeutic effect and mechanism analysis of nimergoline combined with taVNS in the treatment of cognitive impairment in patients with stroke
Chen Lanying; Zhang Xu; Xiao Zhijuan; Yan Kun; Liu Minxiao; Tang Jinsong; Zhao Peng; Li Wei;
2025,40(1):85-88 ,DOI: 10.3969/j.issn.1005-3697.2025.01.019
Abstract:
Objective:To investigate the therapeutic effect and mechanism analysis of nimergoline combined with transcutaneous auricular vagus nerve stimulation(taVNS)in the treatment of post-stroke cognitive impairment(PSCI).Methods:104 PSCI patients were selected as the study subjects and divided into a research group(n=52)and a control group(n=52)according to different treatment methods.The control group was given a conventional treatment plan,while the observation group was given a combination of nimergoline and taVNS on the basis of the control group.After 4 weeks of intervention,the levels of cognitive function,neurological func-tion,neurotransmitters,and inflammatory factors were compared between the two groups of patients.Results:After 4 weeks of interven-tion,compared with the control group,the research group showed an increase in MoCA scores in all dimensions(P＜0.05),a decrease in NIHSS scores,neuron-specific enolase(NSE)and S100 calcium binding protein β(S100β)levels(P＜0.05),an increase in ser-um 5-hydroxytryptamine(5-HT),dopamine(DA),and norepin ephrine(NE)levels(P＜0.05),and a decrease in serum interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels(P＜0.05).Conclusion:The combination of nimergo-line and taVNS has a definite therapeutic effect on PSCI,and it is speculated that its mechanism of action may be related to improving the levels of neurotransmitters and inflammatory factors.
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Clinical effect of vitamin D assisted with methylphenidate in the treatment of comorbid attention deficit hyperactivity disorder in children with benign epilepsy with centrotemporal spikes
2025,40(1):89-93 ,DOI: 10.3969/j.issn.1005-3697.2025.01.020
Abstract:
Objective:To explore the clinical efficacy of vitamin D-assisted methylphenidate in the treatment of children with be-nign epilepsy with centrotemporal spikes(BECTS)complicated with attention deficit hyperactivity disorder(ADHD).Methods:60 children with BECTS and ADHD were selected as the research subjects.According to the different treatment methods,they were divided into control group and combined group,30 cases in each group.The control group was treated with methylphenidate,and the observation group was treated with vitamin D combined with methylphenida,continuous treatment for 3 months.The clinical efficacy,clinical core symptoms[Conners Children's Behavioral Parent Questionnaire(PSQ)],cognitive function[Wechsler Intelligence Scale for Children-Ⅳ(WISC-Ⅳ),Mini-Mental State Examination(MMSE)],serum inflammatory factors[interleukin-6(IL-6),IL-1β,tumor necrosis factor-α(TNF-α)],EEG θ/β ratio and incidence of adverse reactions were compared between the two groups.Results:The total effec-tive rate of combined group was higher than that of the control group(93.33％vs.73.33％,P＜0.05).After 3 months of treatment,the PSQ scores of the two groups were decreased(P＜0.05),and the combined group was lower than the control group(P＜0.05).The scores of WISC-Ⅳ and MMSE of children in the two groups were increased(P＜0.05),and those in the combined group were higher than the control group(P＜0.05).The levels of serum inflammatory factors TNF-α,IL-1 β,IL-6 and EEG θ/β ratio of children in the two groups were decreased(P＜0.05),and those in the combined group were lower than those in the control group(P＜0.05).The total incidence of adverse reactions between the two groups had no significant difference(6.67％vs.13.33％,P＞0.05).Conclu-sion:Vitamin D supplementation on the basis of methylphenidate in the treatment of BECTS children with ADHD can improve the clini-cal efficacy,relieve the clinical core symptoms,improve the cognitive function,reduce the level of inflammation and EEG θ/β ratio,and has good safety.
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Analysis of influencing factors of conversion to cesarean section in com-bined spinal-epidural analgesia and establishment of nomogram prediction model
Zhang Qing; Chen Jingfeng; Wang Li; Zhao Huaibiao; Ma Xiangqiong; Wu Shan; Xian Shuanglong;
2025,40(1):94-98 ,DOI: 10.3969/j.issn.1005-3697.2025.01.021
Abstract:
Objective:To explore the risk factors of failure of vaginal trial delivery and transfer to cesarean section in parturients with combined spinal-epidural analgesia,and to construct its nomogram prediction model.Methods:A total of 512 parturients who un-derwent combined spinal-epidural analgesia were included.According to the final delivery method,the patients were divided into vaginal delivery group(n=483)and cesarean section group(n=29).Multivariate Logistic regression analysis was used to establish the inde-pendent risk factors for the failure of vaginal trial production and conversion to cesarean section in parturients undergoing combined spi-nal-epidural analgesia.The nomogram prediction model was constructed,and the receiver operating characteristic(ROC)curve was used to verify the prediction efficiency of the model.Results:There were significant differences in age,BMI at delivery,amniotic fluid indexing,eclampsia and preeclampsia,gestational diabetes mellitus/gestational diabetes mellitus,lower uterine segment muscle wall thickness,Bishop score and HAD score between the two groups(P＜0.05).Logistic regression analysis showed that advanced age,high BMI at delivery,eclampsia and preeclampsia,low Bishop score and high HAD score were independent risk factors for conversion to ce-sarean section after failure of vaginal trial production in combined spinal-epidural analgesia(P＜0.05).ROC curve analysis showed that the nomogram model constructed based on the independent risk factors mentioned above predicted an AUC of 0.835(95％CI:0.800～0.866)for cesarean section during delivery with lumbar epidural analgesia,with a sensitivity and specificity of 89.66％and 67.08％,respectively.Conclusion:Advanced age,high BMI at delivery,combined with eclampsia and preeclampsia,low Bishop score and high HAD score are independent risk factors for conversion to cesarean section after failure of vaginal trial production in parturients undergoing combined spinal-epidural analgesia.The nomogram model constructed based on the above factors has good predictive value.
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Influence of balloon eustachian tuboplasty combined with tympanotomy tube insertion on auditory threshold and eustachian tube function in pa-tients with refractory otitis media with effusion
2025,40(1):99-102 ,DOI: 10.3969/j.issn.1005-3697.2025.01.022
Abstract:
Objective:To explore the influence of balloon eustachian tuboplasty(BET)combined with tympanotomy tube inser-tion(TTI)on auditory threshold and eustachian tube function in patients with refractory otitis media with effusion(OME).Methods:120 patients with refractory OME were selected as the research subjects.According to the different surgical methods,the patients were divided into TTI group(TTI+eustachian tube irrigation)and TTI+BET group(BET combined with TTI+eustachian tube irriga-tion),with 60 cases in each group.Both groups were followed up for 12 months after surgery.The clinical symptoms,complications,hearing threshold[air conduction(AC)threshold,bone conduction(BC)threshold]before surgery and at 1 month after surgery,eu-stachian tube function(ETDQ-7)and pain(VAS)before surgery and at 1,6 and 12 months after surgery and recurrence status after 1 year of follow-up were compared between the two groups.Results:The disappearance time of middle ear effusion,disappearance time of tinnitus and hearing recovery time in TTI+BET group were shorter than those in TTI group(P＜0.05).There was no statistically sig-nificant difference in the overall incidence of postoperative complications between the two groups(P＞0.05).At 1 month after surgery,AC and BC were decreased in both groups(P＜0.05),and the TTI+BET group had lower AC and BC than TTI group after treatment(P＜0.05).At 1,6 and 12 months after surgery,ETDQ-7 score and VAS score in both groups were lower than those before surgery(P＜0.05),and ETDQ-7 score and VAS score in TTI+BET group at each time period after surgery were lower than those in TTI group(P＜0.05).The recurrence rate was lower in TTI+BET group than that in TTI group(P＜0.05).Conclusion:BET combined with TTI and eustachian tube irrigation can improve the clinical efficacy,improve the eustachian tube dysfunction of refractory OME,and re-duce the hearing threshold,and it has low recurrence rate.
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Clinical study of different doses of dexmedetomidine combined with remifentanil for patients with total knee anthroplasty
Cui Shengkun; Party Hongjun; Wu Di; Zhang Hailong;
2025,40(1):103-107 ,DOI: 10.3969/j.issn.1005-3697.2025.01.023
Abstract:
Objective:To evaluate the sedative effect of different doses of dexmedetomidine(Dex)combined with remifentanil(REM)under continuous epidural anesthesia(CEA)in total knee anthroplasty(TKA).Methods:125 patients who received TKA were included and divided into group A(n=42),group B(n=42)and group C(n=41)according to the different doses of Dex dur-ing anesthesia.On the basis of CEA,the three groups were intravenously pumped with 0.4,0.6 and 0.8 μg/kg of Dex respectively,and they were combined with 0.5 μg/kg of REM.The vital signs[mean arterial pressure(MAP),heart rate(HR)],sedation degree[bis-pectral index(BIS)]and stress response[plasma cortisol(Cor)]at the time of entering the room(T0),CEA puncture(T,),skin in-cision(T2),during 1 hour of surgery(T3)and at the end of surgery(T4)were compared between the groups,and the adverse reac-tions were observed.Results:The MAP at T1～T4 in the three groups was lower than that at T0(P＜0.05).Except for no statistically significant difference in HR between group B and group A at T4(P＞0.05),the MAP and HR of patients in all other time points were as follows:group C＜group B＜group A(P＜0.05).At T1～T4,the BIS values of each group were lower than those at T0.At T,,the BIS values of group B and group C were lower than those of group A(P＜0.05),but there was no statistically significant difference in BIS values between group B and group C(P＞0.05).At time T2～T4,the BIS values of group C were lower than those of group B and group A(P＜0.05).There was no statistically significant difference in BIS values between group B and group A(P＞0.05).At T2 and T4,the Cor values of all groups of patients were lower than T0.At T2,the Cor values of group C were lower than those of group B and group A.At T4,the Cor values of group B and group C were lower than those of group A(P＜0.05),there was no statistically sig-nificant difference in the Cor values between group B and group C(P＞0.05).The total incidence of adverse reactions in group C was higher than that in group B(P＜0.05),there was no statistically significant difference between group A and group B and C(P＞0.05).Conclusion:0.6 pg/kg Dex combined with REM under CEA has a good sedative effect on patients with TKA,and it can im-prove the hemodynamics and reduce the stress response,and has high safety.
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The concentration and influencing factors of serum 25-(OH)D levels of women in early pregnancy in Nanchong
Fan Jinyin; Gan Yanqiong; Zhang Youwen; Li Qiuxia; Fan Bo;
2025,40(1):108-112 ,DOI: 10.3969/j.issn.1005-3697.2025.01.024
Abstract:
Objective:To explore the serum 25-(OH)D levels of early pregnant women in Nanchong and their influencing fac-tors.Methods:1,098 pregnant women living in various regions of Nanchong were selected.The serum 25(OH)D values of early preg-nant women were detected by high performance liquid chromatography-tandem mass spectrometry.The present situation of vitamin D levels in pregnant women and their influencing factors were analyzed.Results:The serum 25-(OH)D level of pregnant women was 43.24±20.53 nmol/L in the early pregnancy.The deficiency rate was 67.85％.As individuals age,the levels of 25-(OH)D showed a gradual rise,peaking in the 35-year-old group.Compared with women without complications in the first trimester,the serum 25(OH)D level of early pregnant women after IVF-ET and with hypothyroidism or immune system diseases was higher(P＜0.05).The increase in body mass index(BMI)and the decrease in serum vitamin A concentration were risk factors for serum 25-(OH)D deficiency in early pregnancy(P＜0.05).Conclusion:The abnormal situation of vitamin among women in early pregnancy in Nanchong is the lack of ser-um 25(OH)D concentration.There are statistically significant differences in serum 25-(OH)D concentration in different ages,so indi-vidualized vitamin supplementation programs can be made for different pregnant women during prenatal examination.Maintaining the normal level of serum vitamin A concentration can reduce the risk of serum 25-(OH)D deficiency.
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Incidence and related factors of parenteral nutrition-associated cholestasis in extremely low birth weight infants
Feng Tingting; Yang Xue; Wang Xiaoli;
2025,40(1):113-116 ,DOI: 10.3969/j.issn.1005-3697.2025.01.025
Abstract:
Objective:To analyze the incidence and related factors of parenteral nutrition(PN)-associated cholestasis(PNAC)in extremely low birth weight infants.Methods:The clinical data of 305 extremely low birth weight infants who were received PN sup-portive treatment were collected retrospectively.The incidence of PNAC was calculated.Infants included were divided into the observa-tion group(with PNAC,n=54)and the control group(without PNAC,n=251).Information of the infants was collected,including gender,gestational age,delivery mode,birth weight,1 min and 5 min Apgar scores,complications(neonatal infection,neonatal anemia,etc.),length of hospital stay,feeding difficulties,PN start time,PN duration,cumulative dosage of amino acids,cumulative dosage of fat emulsion,and the levels of serum indicators[alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBil)and total bile acid(TBA)]on 14 day after birth.Univariate analysis and multivariate analysis were conducted to screen the factors influencing the occurrence of PNAC in extremely low birth weight infants.Results:There were 54 cases of PNAC,with an inci-dence rate of 17.70％.Compared with the control group,gestational age and birth weight of the observation group were lower(P＜0.05).The proportion of neonatal infections,length of hospital stay,the proportion of feeding difficulties,PN duration,and the levels of ALT,AST,TBil and TBA in the observation group were higher and longer(P＜0.05).Logistic regression analysis showed that high birth weight was a protective factor,while PN duration and the levels of ALT,AST,TBil and TBA were independent risk factors for PNAC in extremely low birth weight infants(P＜0.05).Conclusion:Birth weight,PN duration and the levels of ALT,AST,TBil and TBA are helpful for evaluating and predicting the occurrence of PNAC in extremely low birth weight infants.
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Correlation between serum thyroid hormones and cortisol levels and clini-cal symptoms in patients with paranoid schizophrenia
2025,40(1):117-120 ,DOI: 10.3969/j.issn.1005-3697.2025.01.026
Abstract:
Objective:To investigate the correlation of serum thyroid hormones and cortisol(COR)levels with clinical symp-toms in patients with paranoid schizophrenia.Methods:110 patients with paranoid schizophrenia were divided into positive group(n=55)and negative group(n=55)according to the clinical symptoms.In addition,55 healthy people who received physical examination during the same period were included in the control group.Positive and Negative Symptom Scale(PANSS)scores were evaluated and serum thyroid hormones[thyroid stimulating hormone(TSH),free thyroxine(FT4)and free triiodothyronine(FT3)]and COR were detected in the three groups of subjects,and the PANSS scores and levels of thyroid hormones and COR were compared.Pearson correla-tion analysis was used to analyze the correlation between thyroid hormones,COR and PANSS scores in positive group and negative group.Results:The scores of subscales and total score of PANSS in positive and negative groups were higher than those in control group(P＜0.05).The scores of positive symptom and general psychopathological symptom in positive group were higher than those in nega-tive group(P＜0.05)while the score of negative symptom was lower than that in negative group(P＜0.05).TSH level in positive and negative groups was lower(P＜0.05)while FT4,FT3 and COR levels were higher than those in control group(P＜0.05).TSH level in positive group was lower than that in negative group(P＜0.05),but FT4 and FT3 levels were not significantly different from those in negative group(P＞0.05),and COR level was higher than that in negative group(P＜0.05).TSH level in positive group was nega-tively correlated with positive symptom score(P＜0.05),TSH level in negative group was negatively correlated with negative symptom score(P＜0.05),and TSH level in positive and negative groups was negatively corr-elated with general psychopathological symptom score and total score of PANSS(P＜0.05).COR level in positive group was positively correlated with positive symptom score,general psychopathological symptom score and PANSS total score(P＜0.05).Conclusion:Patients with paranoid schizophrenia have abnormal thyroid function and stress function,and the abnormal stress function of patients with positive symptom is more obvious,and serum TSH and COR levels are related to the clinical symptoms of patients.
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Three cases of systemic lupus erythematosus with splenomegaly as the first symptom
Zhao Xin; Tu Huawei; Xie Chuanmei;
2025,40(1):121-122 ,DOI: 10.3969/j.issn.1005-3697.2025.01.027
Abstract:
< positive > 1 case report case 1: the patient, male, 34 years old, was found to have obvious splenomegaly due to abdominal distension, which was not suitable for out of hospital examination. The spleen was 8.2 cm below the rib, and B-ultrasound showed that the length of the spleen was 21.3 cm. After improving the conventional search for the cause of splenomegaly, the cause of splenomegaly could not be determined, and the patient had no other organ or system damage, so splenectomy was performed. Butterfly shaped erythema appeared on the face of the patient 10 days after surgery, so the examination of systemic lupus erythematosus (SLE) related immune indicators was improved.
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Effects of comprehensive nursing based on NRS2002 nutritional assessment in elderly patients with chronic heart failure combined with frailty
Zhang Yanju; Yu Yanjie; Zhao Runping; Wu Huiyuan; Liu Jinzhu; Luo Yuhong;
2025,40(1):123-126 ,DOI: 10.3969/j.issn.1005-3697.2025.01.028
Abstract:
Objective:To investigate the effects of comprehensive nursing based on NRS2002 nutritional assessment on asthenia,nutritional status and ability of daily living in elderly patients with chronic heart failure combined with frailty.Methods:204 elderly pa-tients with chronic heart failure combined with frailty were included and divided into observation group and control group according to different intervention methods,with 102 cases in each group.The control group took routine nursing program,and the observation group took comprehensive nursing program based on NRS2002 nutritional assessment.The intervention time was 6 months,frailty,nutritional status,ability of daily living before and after intervention length of stay and readmission rate were compared between the two groups.Re-sults:After 6 months of intervention,the scores and total scores of somatic,psychological,and social frailty dimensions in Tilburg frailty indicator(TFI)of patients in the observation group was lower than the control group(P＜0.05).The serum albumin(Alb),prealbu-min(PA),transferrin(TF)and hemoglobin(Hb)levels in the observation group were higher than those in the control group(P＜0.05).The scores of the activity of daily living(ADL)in the observation group was higher than that in the control group(P＜0.05).The length of hospital stay in the observation group was shorter than that in the control group(P＜0.05),and the readmission rate dur-ing follow-up was lower than that in the control group(P＜0.05).Conclusion:Comprehensive nursing based on NRS2002 nutritional assessment can improve the frailty degree and nutritional status of elderly patients with chronic heart failure and frailty,improve their daily living ability,shorten the rehabilitation process,and reduce the risk of readmission.
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Effect of standardized nursing mode based on evidence-based concept in high flow humidified oxygen therapy for elderly patients with pneumonia in emergency department
Wang Yin; Liu Hai Lian; Wang Xiaojing; Hu Ying;
2025,40(1):127-131 ,DOI: 10.3969/j.issn.1005-3697.2025.01.029
Abstract:
Objective:To explore the application effect of standardized nursing model based on evidence-based concept in high-flow humidified oxygen therapy(HFNC)for emergency elderly patients with pneumonia.Methods:A total of 160 elderly patients with pneumonia were selected as the study subjects.The patients were divided into control group(n=80)and observation group(n=80)according to different nursing methods.The control group was given HFNC nursing with routine nursing mode,and the observation group was given HFNC nursing with standardized nursing mode based on evidence-based concept.Both groups were intervened for 7 d.The differences of arterial blood gas indexes[arterial partial pressure of oxygen(PaO2),arterial partial pressure of carbon dioxide(PaCO2)],respiration,heart rate,oxygen saturation(SpO2),time of symptom improvement[normal body temperature time,lung moist rale weakening time,sputum thinning time and shortness of breath disappearance time]and adverse reactions were compared between the two groups.Results:After intervention,PaO2 and SpO2 increased in both groups(P＜0.05),and the observation group was higher(P＜0.05).PaCO2,respiration and heart rate decreased in both groups(P＜0.05),and the observation group was lower(P＜0.05).The normal temperature time,lung moist rale weakening time,sputum thinning time and shortness of breath disappearance time in the observation group were shorter than those in the control group(P＜0.05).The incidence of adverse reactions in the observation group was lower than that in the control group(P＜0.05).Conclusion:In emergency elderly patients with pneumonia HFNC,the standard-ized nursing model based on evidence-based concept can improve the blood gas index of patients,correct the symptoms of hypoxia,and promote the improvement of clinical symptoms and reduce the occurrence of adverse reactions of oxygen therapy.
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Effect of nursing model based on"Watson's theory of care"on kinesopho-bia,pain and functional recovery in patients undergoing total knee arthro-plasty
Zhang Ting; Ruan Renzhi; Xu Jin;
2025,40(1):132-136 ,DOI: 10.3969/j.issn.1005-3697.2025.01.030
Abstract:
Objective:To investigate the effect of nursing model based on"Watson's theory of care"on kinesophobia,pain and functional recovery in patients undergoing total knee arthroplasty(TKA).Methods:A total of 100 patients treated with TKA were se-lected as study objects and they were divided into control group(n=50)and study group(n=50)according to different nursing mod-el.The control group adopted the routine nursing mode,and the research group adopted the nursing mode based on the"Watson's theory of care"on the basis of the control group.Postoperative pain,fear degree,knee function and daily living ability of the two groups were compared,and occurrence of complications 5 days after surgery were recorded.Results:The visual analogue scale(VAS)of the study group was lower than that of the control group at 12 h,1,3 and 5 d after surgery(P＜0.05).The score of Tampa kinetophobiascale(TSK)in the study group was lower than that in the control group at 1,3 and 5 d after surgery(P＜0.05),and the incidence of kine-tophobia in the study group was lower than that in the control group at 5 d after surgery(4.00％vs.18.00％,P＜0.05).At 3 and 5 d after surgery,the knee joint score(HSS)of the Hospital for Special Surgery in the study group was higher than that of the control group(P＜0.05).Activity of daily living(ADL)scores in the study group were higher than those in the control group at 3 and 5 d af-ter surgery(P＜0.05).The incidence of complications 5 d after operation in the study group was lower than that in the control group(16.00％vs.36.00％,P＜0.05).Conclusion:The nursing mode based on"Watson's theory of care"is beneficial to alleviate the postoperative pain of TKA patients,promote the recovery of knee function and daily living ability,and reduce the risk of postoperative kinesophobia and complications.
Basic Medical Research
Clinical medical research
Short stories and cases
nursing
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Analysis of Clinical Characteristics, Efficacy, Safety and Short-term and Medium-term Prognosis After Endoscopic Submucosal Dissection in Early Gastric Cancer Patients with Lesions at Different Gastric Sites
Abstract:
Objective To investigate the clinical characteristics of patients with early gastric cancer (EGC) at different lesion sites, and to analyze the postoperative efficacy, safety and medium-term prognosis after endoscopic submucosal dissection (ESD). Methods A total of 104 EGC patients who received ESD treatment in our hospital from January 2021 to December 2024 were enrolled. According to lesion location, the patients were divided into cardia group (5 cases), gastric fundus group (8 cases), gastric body group (9 cases), gastric angular group (29 cases) and gastric antrum group (53 cases). General baseline data, admission diagnosis, pathological risk grade, treatment efficacy, surgical safety and short-term and medium-term prognosis were compared among all groups. Results There were no significant differences in age, composition of admission diagnosis and pathological risk grade (low-risk vs. high-risk pathology) among patients with different lesion sites (all P>0.05). The overall difference in gender composition among groups was at a critical level (Pearson chi-square=9.464, P=0.050). Combined with the results of likelihood ratio chi-square test (likelihood ratio chi-square=11.916, P=0.018), the difference in gender distribution was statistically significant. The en bloc resection rate of ESD was 100.00% in all groups, while significant intergroup differences were found in complete resection rate and curative resection rate (all P<0.05). No significant differences were observed in the incidence of postoperative bleeding and perforation after ESD across groups (all P>0.05). The 1-year survival rate was 100.00% in all groups; no significant difference was detected in the 1-year recurrence rate (P>0.05). Due to the limitation of sample size, the 3-year follow-up data were only analyzed descriptively. The 3-year recurrence rate of the cardia group and gastric fundus group was 100.00%, with a 3-year survival rate of 0.00%, and no death occurred in the other groups. Conclusion The anatomical lesion site of EGC is correlated with gender distribution, postoperative efficacy of ESD and medium-term prognosis. It is recommended to optimize the refined surgical manipulation for lesions in the cardia and gastric fundus, and strengthen targeted postoperative follow-up for patients with cardiac lesions to reduce the risk of tumor recurrence.
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Evaluation of the Efficacy of Procalcitonin-Guided Antibiotic Therapy Strategy in Patients After Gastrointestinal Perforation Surgery
Abstract:
Objective To investigate the efficacy of a procalcitonin (PCT)?guided antibiotic therapy strategy in patients after gastrointestinal perforation surgery. Methods A total of 118 patients who underwent gastrointestinal perforation surgery at our hospital from January 2021 to December 2025 were enrolled. According to the antibiotic therapy strategy, they were divided into a control group (n=59) and an observation group (n=59). The control group received conventional empirical antibiotic discontinuation, while the observation group additionally adopted serum PCT dynamic monitoring. The duration of antibiotic use, postoperative hospital stay, hospitalization costs, postoperative white blood cell (WBC) count and C-reactive protein (CRP) levels, postoperative infection-related complications, and the incidence of newly acquired multidrug-resistant organism (MDRO) infection during hospitalization were compared between the two groups. Results The duration of antibiotic use and postoperative hospital stay were shorter in the observation group than in the control group, and hospitalization costs were lower, with statistically significant differences (P<0.05). At 1 day postoperatively, there were no statistically significant differences in WBC and CRP levels between the two groups (P>0.05). At 5 days postoperatively, both WBC and CRP levels decreased compared with those at 1 day postoperatively in both groups (P<0.05), and the levels in the observation group were lower than those in the control group, with statistically significant differences (P<0.05). There was no statistically significant difference in the incidence of complications (including abdominal infection, wound infection, pulmonary infection, and urinary tract infection) between the two groups (P>0.05). The incidence of newly acquired MDRO infection during hospitalization was lower in the observation group than in the control group, with a statistically significant difference (P<0.05).SConclusion The PCT?guided antibiotic discontinuation strategy after gastrointestinal perforation surgery can effectively shorten the duration of antibiotic use and hospital stay, reduce hospitalization costs, alleviate inflammatory response, and does not increase the risk of infection?related complications, while also helping to reduce the incidence of MDRO infection.
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Influence of high-quality nursing based on Noddings’ caring theory on symptom relief and nursing quality in children with pneumonia
Abstract:
Objective To evaluate the improvement effects of high-quality nursing based on Noddings’ caring theory on symptom relief, blood gas indicators and nursing quality in children with pneumonia. Methods A total of 74 children with pneumonia admitted to the hospital from October 2025 to August 2026 were enrolled as study subjects, and were randomly classified into intervention group and control group, with 37 cases in each group. The control group implemented routine nursing of pediatric pneumonia, whereas the intervention group carried out high-quality nursing intervention based on Noddings’ caring theory on the basis of routine nursing. The differences in symptom relief indicators (fever abatement time, cough disappearance time, lung rale disappearance time, hospitalization time) and blood gas analysis indicators [partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), blood oxygen saturation (SpO2), pH value] before and after intervention were compared between both groups, and the differences in medication compliance, comfort degree [numerical rating scale (NRS)] and nursing satisfaction of family members were recorded and compared. Results The intervention group [(1.74±0.85) d, (3.62±1.03) d, (2.18±1.06) d, (4.08±0.29) d] demonstrated shorter fever abatement time, cough disappearance time, lung rale disappearance time and total hospitalization time compared to the control group [(2.25±1.02) d, (4.19±1.24) d, (2.73±1.11) d, (4.55±0.36) d] (P<0.05). After intervention, the PaO2, SpO2 and pH in the intervention group were significantly higher than those in the control group (P<0.05) while the PaCO2 was significantly lower (P<0.05). Concurrently, the intervention group exhibited significantly lower NRS comfort score compared to the control group [(2.35±1.02) points vs. (3.92±1.45) points] (P<0.05), and also showed significantly higher medication compliance rate and family nursing satisfaction than the control group (97.30% vs. 81.08%, 97.30% vs. 83.78%) (P<0.05). Conclusion High-quality nursing based on Noddings’ caring theory can effectively promote the clinical symptom relief in children with pneumonia, improve blood gas indicators, enhance the treatment comfort and compliance, and significantly enhance the family members’ satisfaction with nursing work.
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Effects of feeding patterns on early development in small for gestational age Infants
Abstract:
Objective To investigate the effects of different feeding methods on the early development of small-for-gestational-age (SGA) infants, and to provide clinical evidence for feeding strategies in SGA children. Methods SGA children born at the Department of Obstetrics, People''s Hospital of Dujiangyan City from April 2022 to April 2025 and systematically managed in the pediatric outpatient clinic were selected. They were divided into exclusive breastfeeding group, mixed feeding group, and formula feeding group according to feeding methods. Appropriate-for-gestational-age (AGA) infants born during the same period were selected as controls. Physical growth and neuropsychological development were compared among groups. Results 1. At 3 months of age, the weight Z-score and incidence of catch-up growth in height, and at 6 months of age, the incidence of catch-up growth in weight were significantly lower in the exclusive breastfeeding group than in the formula feeding group; after 6 months, the differences gradually decreased and showed no statistical significance. 2. The incidence of obesity was significantly lower in the exclusive breastfeeding group than in the formula feeding group. 3. No statistically significant difference was found in head circumference Z-scores. 4. The developmental quotients for language, adaptive ability, and social behavior in the formula-fed SGA group were significantly lower than those in the AGA group and the exclusive breastfeeding group; no statistically significant differences were observed between the exclusive breastfeeding group and the AGA group across all developmental dimensions. Conclusion 1. Exclusive breastfeeding in SGA children aged 0–12 months demonstrates more uniform and sustained catch-up growth with a more stable growth rate. 2. Different feeding methods do not affect the physical catch-up growth outcome of SGA children at 12 months of age, but exclusive breastfeeding is associated with a lower risk of obesity. 3. Most SGA children achieve catch-up growth within the first year of life, especially before 6 months of age. 4. Exclusively breastfed SGA children exhibit higher levels of neuropsychological development, comparable to those of AGA children.
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The efficacy of calcitriol combined with elcatonin in treating osteoporosis and its impact on bone metabolism in patients
Abstract:
Objective: To investigate the efficacy of calcitriol (Cal) combined with elcatonin on osteoporosis (OP) and its impact on bone metabolism in patients. Methods: One hundred elderly OP patients admitted to Huairou District Traditional Chinese Medicine Hospital from June 2022 to June 2024 were enrolled as the research subjects and randomly assigned into criterion group (50 cases) and joint group (50 cases) complying with random number table. The criterion group received oral Cal soft capsules, while the joint group received additional elcatonin injection on top of the criterion group. The treatment course for both groups was 3 months, with a follow-up of 12 months. The efficacy, bone metabolism [bone alkaline phosphatase (BALP), tartrate resistant acid phosphatase (TRACP), osteocalcin (OC), type I collagen cross-linked C-terminal peptide (CTX)], bone mineral density (BMD), bone pain score, quality of life score before treatment and after 3 months of treatment, and incidence of adverse reactions were compared between the two groups. Results The therapeutic effect of the joint group was significantly better than that of the criterion group (94.00%, 70.00%, P<0.05). After treatment, the BALP, OC, BMD, and quality of life scores of both groups significantly increased compared with those before treatment, and the joint group was significantly higher than the criterion group (P<0.05). The TRACP, CTX, and bone pain scores of both groups significantly decreased compared with those before treatment, and the joint group was prominently lower than the criterion group (P<0.05). There was no statistically significant difference in the total incidence of adverse reactions between the two groups (P>0.05). At 12-month follow-up, there was no statistically significant difference in fracture incidence between the two groups (P>0.05). Conclusion: The combination of Cal and elcatonin has good efficacy in treating OP patients. It can regulate bone metabolism indicators, effectively regulate bone metabolism, increase bone density, relieve pain and improve quality of life, and has high safety.
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Microstructural Alterations of the Substantia Nigra and Insula in Parkinson’s Disease Based on Diffusion Tensor Imaging Fractional Anisotropy Features: An Exploratory Machine Learning Analysis
Abstract:
Objective To investigate alterations in diffusion tensor imaging (DTI)-derived fractional anisotropy (FA) of the substantia nigra and insula in patients with Parkinson’s disease (PD), and to explore the internal classification performance and feature contribution of machine learning models based on FA features in a single-center case-control cohort. Methods Thirty-five patients with PD and 28 healthy controls (HCs) were included. Group differences in bilateral substantia nigra FA, bilateral insular FA, and derived FA indices were compared. Linear regression analyses were performed after adjustment for age, sex, and years of education. Elastic net logistic regression, radial basis function kernel support vector machine, and random forest models were constructed using four original FA features. Model performance was evaluated using nested cross-validation, and permutation importance was used to assess ferture contribution. Results Mean FA values of the bilateral substantia nigra, bilateral insula, and the overall average FA were significantly lower in the PD group than in the HC group (all P<0.001), and these differences remained significant after adjustment for covariates. The ACU for discriminating PD from HC using mean bilateral substantia nigra FA was 0.919. In nested cross-validation, the radial basis function kernel support vector machine achieved an AUC of 1.000. Permutation importance indicated that FA values of the right and left substantia nigra contributed more substantially to model discrimination. Conclusion In this single-center case-control cohort,ROI-FA was reduced in both the substantia nigra and insula in patients with PD, with more robust between-group differences observed in substantia nigra FA.
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Distribution of virulence genes in GBS causing infection in the third trimester of pregnancy and its effects on vaginal microecology and maternal-neonatal outcomes
Abstract:
Objective: To investigate the distribution of virulence genes in group B Streptococcus (GBS) causing infection in the third trimester of pregnancy and its effects on vaginal microecology and maternal-neonatal outcomes. Methods: A total of 200 pregnant women who had GBS infection in the third trimester and were admitted to our hospital from December 2021 to December 2025 were selected as the infection group. Meanwhile, 200 GBS-negative pregnant women in the third trimester were selected as the control group. Polymerase chain reaction was used to analyze the distribution of virulence genes in GBS. Vaginal microecology and maternal-neonatal outcomes were compared between the two groups. Results: In the 200 GBS strains, the most prevalent virulence genes were hyl B (100.00%), cyl E (100.00%), and scp B (96.50%), which were detected in different serotypes. The detection rate of lmb was 66.50%, and it was common in serotypes II, VI, and IX. The distribution of bac and bca was limited, with detection rates of 4.50% and 10.50%, respectively. The proportions of vaginal microecological imbalance, abnormal vaginal pH, and grades III–IV vaginal cleanliness in the infection group were all higher than those in the control group (P<0.05). The rate of cesarean section was higher (P=0.002), and the rate of natural vaginal delivery was lower in the infection group than in the control group (P=0.001). The incidences of intrauterine infection, premature rupture of membranes, puerperal infection, neonatal asphyxia, and neonatal pneumonia were all higher in the infection group (P<0.05). Conclusion: Virulence genes in GBS causing infection in the third trimester of pregnancy mainly include hyl B, cyl E, and scp B. GBS infection can lead to vaginal microecological imbalance and increase the risk of adverse maternal-neonatal outcomes.
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Adaptive Modal Gated Cross-Attention Fusion Model for Non-invasive Prediction of MGMT Promoter Methylation in Adult Diffuse Gliomas
刘高元, 张勇, 李卫星, 丁兀兀, 钱爽, 邓成苗, 陈琳
Abstract:
Objective: To develop an Adaptive Modality Gating Multi-Head Cross-Attention Fusion Model (AM-GMCA-Swin-S) based on Swin-S Transformer and explore its application value in non-invasive prediction of MGMT methylation status. Materials and Methods: A retrospective cohort of 328 adult diffuse glioma patients admitted to Deyang People’s Hospital from January 2011 to January 2025 was collected, including 230 cases with MGMT methylation positive and 98 cases with negative status, for model training and internal validation. A total of 82 cases selected from the publicly available multi-center ReMIND dataset were used as an independent external test set (59 methylation positive, 23 negative). After standardized preprocessing of T2WI, DWI, and 3D-T1CE images, 27-channel 2.5D image matrices covering axial, coronal, and sagittal planes were constructed. Clinical features, intratumoral and peritumoral radiomic features were extracted simultaneously. Four comparative models were established: semantic feature baseline model (LR), pure imaging modal baseline model (Native Swin-S), fixed-weight cross-attention fusion model (FW-CA-Swin-S), and AM-GMCA-Swin-S model. Multiple indicators, including PR-AUC, ROC-AUC, sensitivity, specificity, and F1-score were adopted to evaluate model discrimination efficacy. DeLong test was used to compare inter-group differences in AUC values, and continuous net reclassification improvement (continuous NRI) as well as integrated discrimination improvement (IDI) were calculated to quantify reclassification benefits. Results: Stratified 5-fold internal cross-validation demonstrated that the LR baseline model achieved the optimal overall discriminative performance, while AM-GMCA-Swin-S obtained the highest specificity. In independent external validation, AM-GMCA-Swin-S exhibited the best comprehensive performance, with PR-AUC of 0.856, specificity of 0.696, sensitivity of 0.678, and F1-score of 0.755. Its sample reclassification and risk stratification capacity were significantly superior to FW-CA-Swin-S (P<0.05). Compared to the LR baseline model (PR-AUC = 0.830), although there was no statistically significant difference in overall discriminative performance (P>0.05), AM-GMCA-Swin-S showed better individualized risk stratification. The Native Swin-S model had the worst discriminative performance and exhibited severe prediction bias. Conclusions: AM-GMCA-Swin-S can dynamically adjust the weights of different modality features es based on the tumor heterogeneity of individual samples, achieving adaptive fusion of multi-modal information and significantly improving the model’s comprehensive predictive performance. It remarkably enhances sample reclassification and individualized risk stratification capabilities, offering a new technical reference for the non-invasive assessment of MGMT methylation status and individualized diagnosis and treatment in adult diffuse gliomas.
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Comparison of the efficacy of different antiviral schemes in the prevention and treatment of chronic hepatitis B low-level viremia
Abstract:
Objective: This study aims to systematically compare the efficacy differences of different antiviral regimens in preventing and treating low-level viremia in patients with chronic hepatitis B. Methods: (1) This study retrospectively included 734 CHB patients diagnosed and treated in the infectious diseases Department of our hospital from January 2023 to June 2024. They were divided into non first-line NAs group of 48 cases, first-line NAs group of 585 cases, and combined treatment group of 101 cases. The first-line NAs group was divided into ETV subgroup (347 cases), TDF subgroup (140 cases), and TAF subgroup (98 cases). (2) Analysis of independent influencing factors on LLV and HBsAg clearance.Compare the differences in therapeutic efficacy among different groups and observe and analyze the dynamic evolution of virological and serological markers during Peg-IFN α-2b treatment. Result: High baseline HBV DNA, high HBsAg, HBeAg positivity, liver cirrhosis, and hepatocellular carcinoma are independent risk factors for LLV.There was a significant difference in the incidence of LLV among the three groups (χ ²=72.34, P<0.001): the non first-line NAs group had the highest incidence of LLV, reaching 47.92%; The first-line NAs group is second, accounting for 32.65%; The combination therapy group had the lowest rate, only 3.96%. The CVR rate of the combination therapy group showed a continuous upward trend with prolonged treatment time, with outstanding performance in the dynamic decline of HBsAg. Conclusion: There are significant differences in the prevention and treatment efficacy of different antiviral regimens for LLV, and TAF can be the preferred choice among first-line NAs;Combined interferon therapy is an independent protective factor. Interferon has made "functional cure" an accessible target in advantageous populations. Clinical doctors should have a correct understanding of the transient HBV DNA elevation during interferon treatment and adhere to full course treatment.
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A retrospective study to explore the factors related to the rehabilitation of patients after laparoscopic ovarian cystectomy
Abstract:
目的：探析卵巢囊肿患者接受多孔腹腔镜卵巢囊肿剥除术后的康复效率，并分析影响康复的相关因素。方法：纳入182例多孔腹腔镜卵巢囊肿剥除术患者进行回顾性研究，根据住院时间将患者分为康复正常(≤5 d)与康复延迟(>5 d)，并分析影响术后康复的相关因素。结果：康复延迟与康复正常患者在年龄、术后活动时间、术前营养不佳、囊液外溢、临近脏器损伤、感染预防占比上存在统计学差异(P<0.05)；Logistic回归分析显示：年龄、术后活动时间、术前营养不佳、囊液外溢、临近脏器损伤是康复延迟的相关因素(P<0.05)，以此构建预测模型的AUC为0.857。结论：多孔腹腔镜手术后康复可能与医源性损伤、营养状态、年龄、术后活动时间等有关，临床可根据本研究中的可控因素制定干预策略，以促进术后康复。
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Therapeutic effect of modified Twin-block appliance combined with micro-implant anchorage in patients with Angle Class II division 1 malocclusion
Abstract:
Objective： To investigate the therapeutic effect of modified Twin-block appliance combined with micro-implant anchorage in patients with Angle Class II division 1 malocclusion. Methods： A retrospective analysis was conducted on the clinical data of 125 patients diagnosed with Angle Class II division 1 malocclusion. According to the treatment method, patients were divided into the control group (modified Twin-block appliance alone, n=61) and the observation group (modified Twin-block appliance combined with micro-implant anchorage, n=64). Clinical efficacy, cephalometric measurements of soft and hard tissues, severity of malocclusion, periodontal indicators, satisfaction, and complications were compared between the two groups. Results： The observation group demonstrated a significantly superior overall effective rate compared to the control group (95.31% vs. 81.97%, P<0.05).Post-treatment assessments revealed that the observation group exhibited smaller ANB, U1-NA, and U1-SN values, alongside larger L1-MP and Z-angle measurements. Furthermore, this group showed lower upper lip protrusion (ULP), lower lip protrusion (LLP), and Peer Assessment Rating (PAR) scores than the control group (P<0.05). The observation group reported a significantly higher satisfaction rate compared to the control group (96.88% vs. 80.33%, P<0.05). Conclusion： Modified Twin-block appliance combined with micro-implant anchorage is effective in treating Angle Class II division 1 malocclusion. It can effectively improve soft and hard tissue indicators and the severity of malocclusion, and enhance patient satisfaction.
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Concentrated growth factor for alveolar bone defect repair after mandibular impacted third molar extraction
Abstract:
目的：探讨下颌阻生第三磨牙拔除术后应用浓缩生长因子(CGF)修复第二磨牙远中牙槽骨缺损的临床疗效。方法：选取126例行下颌阻生第三磨牙拔除术后患者，按治疗方案分为CGF组和对照组，各63例。对照组拔牙窝清创后直接缝合，CGF组在此基础上填充CGF。比较两组第二磨牙远中骨缺损深度(ODD)、牙周指标、术后疼痛视觉模拟评分(VAS)、面部肿胀程度，记录术后并发症情况。结果：术后3、6个月，CGF组牙周探诊深度、ODD及临床附着水平改善均优于对照组(P<0.05)，CGF组ODD变化量及减少率均高于对照组(P<0.05)；术后3、7 d，CGF组VAS评分及面部肿胀程度低于对照组(P<0.05)；两组并发症总发生率无明显差异(P>0.05)。结论：下颌阻生第三磨牙拔除术后使用CGF修复牙槽骨缺损效果良好，改善邻牙牙周状况，减轻术后中后期疼痛与面部肿胀。
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Visual Analysis of Traditional Chinese Medicine Treatment for Breast Cancer-Related Fatigue Based on Data Mining
李梦婷, 薛晓红, 陈玮黎, 张馨月, 李思雨, 沙珊焱, 徐董韵
Abstract:
目的：运用文献计量学分析中医药治疗乳腺癌癌因性疲乏(CRF)的研究现状、热点及趋势。方法：检索中英文文献数据库的相关文献，采用CiteSpace分析作者、机构及关键词。结果：共纳入文献248篇，中医药治疗乳腺癌CRF研究领域发文量总体呈上升趋势，任莉莉发文最多，中国中医科学院广安门医院、山东中医药大学和辽宁中医药大学为主要机构。关键词中，中文文献中“乳腺癌”、“化疗”、“睡眠治疗”排名前3；英文文献中“cancer-related fatigue”“breast cancer”“traditional chinese medicine”排名前3。研究热点包括耳穴压丸、中药浴足、八珍汤、太极拳及睡眠质量、负性情绪改善。中文文献侧重临床应用与名医经验，英文文献侧重基础实验。结论：中医药治疗乳腺癌CRF领域研究持续发展，未来将趋向中药多靶点机制、分子对接、网络药理学及网状Meta分析等高质量循证研究。
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Clinical Efficacy of Tranexamic Acid and Carboprost Tromethamine Combined with Intrauterine Balloon in the Treatment of Postpartum Hemorrhage and Their Effects on Endometrial Repair
Abstract:
Objective: To compare the clinical efficacy of tranexamic acid, carboprost tromethamine combined with intrauterine balloon versus carboprost tromethamine combined with intrauterine balloon in the treatment of postpartum hemorrhage (PPH) and their effects on endometrial repair. Methods: Clinical data of 110 patients with PPH admitted to the Department of Obstetrics, Nanjing Gaochun People''s Hospital from January 2023 to January 2026 were retrospectively analyzed. The observation group (n=55) received carboprost tromethamine, tranexamic acid plus intrauterine balloon tamponade, while the control group (n=55) received carboprost tromethamine plus intrauterine balloon tamponade. The two groups were compared in terms of hemostatic efficacy (intraoperative blood loss, 2-hour and 24-hour postpartum blood loss, hemostasis time, and hemorrhage control rate), endometrial repair parameters (endometrial thickness at 42 days postpartum, lochia duration, and incidence of intrauterine adhesions), and adverse reactions. Results: The observation group demonstrated significantly lower intraoperative blood loss, 2-hour and 24-hour postpartum blood loss, and shorter hemostasis time compared with the control group (all P<0.05), along with a significantly higher hemorrhage control rate (P<0.05). At 42 days postpartum, the observation group showed significantly greater endometrial thickness (P<0.05), significantly shorter lochia duration (P<0.05), and a significantly lower incidence of intrauterine adhesions (P<0.05) relative to the control group. No statistically significant difference was observed in the overall incidence of adverse reactions between the two groups (P>0.05). Conclusion: Carboprost tromethamine, tranexamic acid combined with intrauterine balloon achieves superior hemostatic efficacy compared with carboprost tromethamine combined with intrauterine balloon in the management of PPH, as evidenced by greater reduction in blood loss, shorter hemostasis time, and higher hemorrhage control rate. Moreover, this regimen more effectively promotes postpartum endometrial repair and is associated with a lower incidence of intrauterine adhesions, with a favorable safety profile.
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Correlation between serum TRIM22 and intestinal barrier function markers and the incremental value of TRIM22 in assessing disease activity in patients with ulcerative colitis
Abstract:
Objective: To investigate the correlation between serum tripartite motif-containing protein 22 (TRIM22) and intestinal barrier function markers [D-lactate, diamine oxidase (DAO), and endotoxin] in patients with ulcerative colitis (UC), and to evaluate the incremental value of TRIM22 in assessing disease activity.Methods: A total of 120 UC patients admitted from June 2024 to June 2026 were enrolled as the observation group, and 60 healthy individuals undergoing physical examination during the same period were selected as the control group. Serum levels of TRIM22, D-lactate, DAO, and endotoxin were compared between the two groups. UC patients were divided into remission, mild, moderate, and severe groups according to the Mayo score, and the above indicators were compared among patients with different disease activity. Pearson correlation analysis was used to analyze the correlations of TRIM22 with intestinal barrier markers and Mayo score. With Mayo score ≥6 defined as moderate-to-severe activity, a multivariable logistic regression model was constructed to evaluate the incremental value of TRIM22 in assessing disease activity. Results: Serum TRIM22, D-lactate, DAO, and endotoxin levels in the UC group were significantly higher than those in the control group (P<0.05). Among 120 UC patients, 29 were in remission, 37 had mild disease, 31 had moderate disease, and 23 had severe disease. With increasing disease activity, serum TRIM22, D-lactate, DAO, and endotoxin levels increased gradually (P<0.05). Pearson correlation analysis showed that serum TRIM22 was positively correlated with D-lactate, DAO, endotoxin, and Mayo score in UC patients (all P<0.05), and D-lactate, DAO, and endotoxin were also positively correlated with Mayo score (all P<0.05). The moderate-to-severe group had significantly higher serum TRIM22, D-lactate, DAO, and endotoxin levels than the non-moderate-to-severe group (P<0.05). Logistic regression analysis showed that TRIM22, D-lactate, DAO, and endotoxin were independent factors associated with moderate-to-severe activity (P<0.05). ROC curve analysis showed that the area under the curve (AUC) of TRIM22 combined with D-lactate, DAO, and endotoxin for assessing moderate-to-severe UC was 0.938, with a sensitivity of 87.70% and a specificity of 90.90%, which was higher than that of each indicator alone.Conclusion: Serum TRIM22 is closely associated with intestinal barrier function markers and disease activity in UC patients. Combined detection can improve the performance of disease activity assessment and has incremental value.
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Ultra-early intervention with edaravone dexborneol combined with mecobalamin on executive function and SWI cerebral microbleed evolution after thrombolysis in acute cerebral infarction
Abstract:
Objective: To investigate the effects of edaravone dexborneol combined with mecobalamin on executive function, the evolution of cerebral microbleeds (CMBs) on susceptibility-weighted imaging (SWI), neurological deficits, and activities of daily living in patients with acute cerebral infarction (ACI) after intravenous thrombolysis. Methods: A total of 110 ACI patients who underwent thrombolysis in our hospital from January 2022 to December 2024 were enrolled and randomly divided into a control group and an observation group, with 55 cases in each group. During the study, 2 cases were excluded, leaving 55 cases in the control group and 53 in the observation group who completed the study. The control group received standard treatment, while the observation group received edaravone dexborneol injection and mecobalamin tablets in addition to standard treatment. Executive function was assessed using the Trail Making Test (TMT) B-A time and the Stroop Color-Word Test (SCWT) interference effect at baseline, 14 days, and 90 days after treatment. SWI was performed to record the total number of CMBs and the number of new CMBs, and the proportion of patients with new CMBs was calculated. The National Institutes of Health Stroke Scale (NIHSS), modified Rankin Scale (mRS), and Barthel Index (BI) were also evaluated at each time point. Results: At 14 and 90 days after treatment, the TMT B-A time and SCWT interference effect in the observation group were significantly lower than those in the control group (all P<0.05). Repeated measures analysis of variance revealed significant main effects of time and group, as well as a significant time × group interaction effect for both the TMT B-A time and the SCWT interference effect (all P<0.05), with a greater improvement magnitude in the observation group than in the control group. At 14 and 90 days, the number of new CMBs in the observation group was significantly lower than that in the control group (both P<0.05). Within the control group, the number of new CMBs at 90 days increased significantly compared with that at 14 days (P<0.05), whereas no statistically significant difference was observed within the observation group (P>0.05). At 90 days, the proportion of patients with ≥1 new CMBs in the observation group was significantly lower than that in the control group (P<0.05). At 14 and 90 days, the NIHSS scores in the observation group were significantly lower than those in the control group (both P<0.05). At 90 days, the proportion of patients with an mRS score ≤2 (86.79% vs. 65.45%) and the BI scores in the observation group were significantly higher than those in the control group (both P<0.05). There were no statistically significant differences in the incidence of hemorrhagic transformation or adverse events between the two groups (P>0.05). Conclusion: Ultra-early intervention with edaravone dexborneol combined with mecobalamin can significantly improve executive function and neurological outcomes, attenuate the development of new CMBs, and enhance long-term quality of life in ACI patients after thrombolysis, without increasing the risk of bleeding.
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Clinical Efficacy Observation of Platelet-Rich Plasma Therapy for Knee Osteoarthritis Based on Inflammatory and Matrix Metabolism Regulation
Abstract:
Objective: To asses the clinical efficacy of platelet-rich plasma (PRP) for knee osteoarthritis (KOA), explore its mechanism of action based on inflammation and matrix metabolism regulation, and analyze the impact of PRP quality parameter heterogeneity on therapeutic outcomes. Methods: 80 patients with KOA in the Department of Orthopedics and Pain Medicine at Shijiazhuang People''s Hospital from July 2022 to October 2024 were selected. The 80 KOA patients were randomly divided into a hyaluronic acid (HA) group (n=40) and a platelet-rich plasma (PRP) group (n=40) using a random number generation method. The PRP group received intra-articular injection of PRP into the knee joint cavity once weekly for a total of 3 sessions, while the HA group received intra-articular injection of HA into the knee joint cavity once weekly for a total of 3 sessions. The visual analog scale (VAS) for pain and the Western Ontario and Montreal Arthritis Clinic (WOMAC) score were compared between the two groups before treatment and at 1 month, 3 months, and 6 months post-treatment. Inflammatory cytokines (IL-1β, TNF-α, IL-6) and key proteins of the NF-κB pathway (p-p65, IκBα) in synovial fluid were measured before and after treatment. The correlation between platelet count, platelet distribution width (PDW), mean platelet volume (MPV), and growth factor concentrations in the PRP group and clinical efficacy as well as pathway biomarker changes was analyzed. Results: VAS and WOMAC scores were lower in the PRP group at all time points (P<0.05). At 6 months, synovial IL-1β, TNF-α, IL-6, and p-p65 levels were significantly reduced from baseline in both groups (P<0.05), while the expression levels of IκBα were significantly increased (P<0.05). Moreover, the improvement in these parameters was significantly greater in the PRP group compared to the HA group, with statistically significant differences (P<0.05). Pearson correlation analysis revealed that all quality parameters of PRP were significantly positively correlated with the WOMAC improvement rate (P<0.05). Platelet count (PLT) and TGF-β correlated negatively with ΔIL-1β (P<0.05) and significantly positively correlated with △IκBα (P<0.05), respectively. PLT, TGF-β, and VEGF were significantly negatively correlated with △p-p65 (P<0.05). Conclusion: PRP may exert anti-inflammatory and joint-protective effects by inhibiting the NF-κB pathway, demonstrating superior therapeutic efficacy compared to hyaluronic acid. Its therapeutic effects are influenced by PRP heterogeneity, providing theoretical basis and guidance for the standardized clinical application of PRP in the treatment of KOA.
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DGCR8 promotes melanoma progression by regulating AKT phosphorylation via BCKDHA
Abstract:
Objective:To investigate the effects of DGCR8 on the proliferation, migration, invasion and tumorigenic ability of melanoma cells and its downstream pathway. Methods:Public datasets, including GSE115978, GSE72056 and TCGA-SKCM, were integrated to analyze the expression characteristics, proliferation-related features and prognostic significance of DGCR8 in melanoma. A DGCR8 knockdown model was established in A375 cells, followed by RNA sequencing and enrichment analysis to identify differentially enriched pathways and candidate molecules after DGCR8 knockdown. BCKDHA knockdown and BCKDHA rescue models in the context of DGCR8 knockdown were constructed. mRNA and protein expression, as well as cell proliferation, migration and invasion, were examined. The NAD?/NADH ratio was detected using the WST-8 method, and a nude mouse xenograft assay was performed to evaluate the effect of BCKDHA rescue on the phenotype induced by DGCR8 knockdown. Results:Single-cell sequencing database analysis showed that DGCR8 was highly expressed in melanoma cells and was associated with active cell proliferation. Deconvolution-based survival analysis showed that high DGCR8 expression was associated with poor prognosis. Cellular experiments showed that DGCR8 knockdown inhibited the proliferation, migration and invasion of A375 cells, and led to decreased AKT phosphorylation at Ser473 and Thr308, an increased NAD?/NADH ratio and downregulated BCKDHA expression. BCKDHA rescue restored AKT phosphorylation, proliferation, migration and invasion in DGCR8-knockdown cells and enhanced their tumorigenic ability in vivo. After BCKDHA knockdown, AKT phosphorylation decreased and the NAD?/NADH ratio increased, showing the same direction of change as that observed after DGCR8 knockdown. Conclusion:Our study supports that DGCR8 can regulate AKT phosphorylation and NADH metabolism via BCKDHA, thereby promoting the proliferation, migration, invasion, and tumorigenesis of A375 cells.
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Exploring the Association Between a Novel Nutritional Index (TCBI) and Diabetes Risk Based on the
代金茂, 张哲, 马晓虎, 谢长春, 罗恒希, 李寒露, 雷枭
Abstract:
Objective: Based on the "phlegm-dampness" theory of Traditional Chinese Medicine (TCM), this longitudinal study utilized data from the China Health and Retirement Longitudinal Study (CHARLS) to investigate the association between a novel nutritional index—triglyceride-total cholesterol-body mass index (TCBI)—and the risk of diabetes in middle-aged and older adults. Methods: Data were obtained from the CHARLS database (2011–2020). Cox regression was employed to examine the longitudinal association between TCBI and diabetes risk. Kaplan-Meier curves, restricted cubic splines, receiver operating characteristic (ROC) curves, and subgroup analyses were used for validation. Results: A total of 8,765 Chinese participants aged ≥45 years were included in this study. Cox regression analysis revealed that, after adjusting for covariates, TCBI was longitudinally associated with diabetes risk (HR: 1.38, 95% CI: 1.29–1.48).This finding aligns with the TCM "phlegm-dampness" theory, which posits that "internal accumulation of turbid phlegm and spleen dysfunction" contribute to the development of wasting-thirst disorder (diabetes). Restricted cubic spline analysis indicated a linear relationship between TCBI exposure levels and diabetes risk (P < 0.001). Kaplan-Meier curves demonstrated that individuals with higher TCBI levels had a greater cumulative incidence of diabetes. Subgroup analyses revealed interactions between TCBI and both marital status and history of hypertension (P < 0.05). ROC curve analysis showed that TCBI had a predictive efficacy of 0.64 for diabetes in the middle-aged and older population. Conclusion: TCBI level exhibits a significant positive linear association with diabetes risk in middle-aged and older adults, providing modern epidemiological support for the TCM theory that "phlegm-dampness causes wasting-thirst disorder."
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Influence and role mechanism of CHMP4C on the tumor biological behaviors of Fadu cells in hypopharyngeal squamous cell carcinoma
Abstract:
Background : Hypopharyngeal squamous cell carcinoma is one of the malignant tumors with high incidence in the head and neck. It has the characteristics of strong invasiveness, early occult metastasis, and clinical diagnosis mostly in the middle and late stages. At present, the comprehensive treatment plan based on surgery combined with radiotherapy and chemotherapy has limited benefits. It is urgent to explore the key molecules that regulate tumor progression as potential targeted therapeutic targets. Objective: To explore the influence of CHMP4C on the proliferation, migration and invasion abilities of Fadu cells in hypopharyngeal squamous cell carcinoma. Methods: Fadu cell model with stable knockdown of CHMP4C (shCHMP4C group) and negative control group (shNC group) were constructed. Western Blot was applied to verify the knockdown efficiency of CHMP4C protein. CCK-8 assay was utilized to detect the cell proliferation activity. Transwell migration assay and Matrigel invasion assay were adopted to evaluate the cell migration and invasion abilities. The expression level of epithelial-mesenchymal transition (EMT) key marker E-cadherin was detected by Western Blot. Results: Western Blot analysis revealed significantly reduced CHMP4C protein expression in the shCHMP4C group compared to the shNC group (P<0.05). CCK-8 assay demonstrated markedly slowed cell proliferation in the shCHMP4C group, with lower OD values at 48 h and 72 h compared to the shNC group (P<0.05). Transwell migration and invasion assays suggested reduced transmembrane cell counts in the shCHMP4C group compared with those in the shNC group (P<0.05). Western Blot detection found upregulation of E-cadherin protein expression in the shCHMP4C group relative to the shNC group (P<0.05). Conclusion: CHMP4C can promote the proliferation, migration and invasion abilities of Fadu cells in hypopharyngeal squamous cell carcinoma, which may be achieved by regulating the EMT process. CHMP4C may serve as a potential candidate molecular target for targeted therapy of hypopharyngeal squamous cell carcinoma, although its clinical value requires further investigation.
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A Case of Reactive Cutaneous Capillary Proliferation Induced by Camrelizumab
Abstract:
Objective: To summarise the key points in the diagnosis and management of reactive cutaneous capillary proliferation (RCCEP) induced by the combination of camrelizumab and regorafenib in the treatment of hepatocellular carcinoma, and to outline management strategies in light of the latest research advances. Methods: We reviewed the complete clinical, dermatoscopic and pathological data of a 56-year-old patient with hepatocellular carcinoma and diabetic nephropathy. Cutaneous immune-related adverse event grade was assessed in accordance with Multidisciplinary Expert Consensus on Cutaneous Adverse Reactions Induced by Immune Checkpoint Inhibitors (2025 Edition). The patient underwent surgical excision of large skin lesions and cryotherapy with liquid nitrogen for microscopic lesions, and was treated topically with mupirocin ointment, with a one-year follow-up. Results: Following four cycles of immunotherapy combined with anti-vascular therapy, the patient developed multiple, easily bleeding, red, pedunculated papules on the posterior neck and trunk; dermoscopy revealed characteristic vascular lacunae and the ‘white curtain’ sign, whilst histopathology demonstrated benign lobular capillary proliferation in the dermis. The lesion was graded as grade 2 RCCEP; after local treatment, there was no recurrence of the lesions at the one-year follow-up. Underlying diabetic microvascular damage exacerbated the haemorrhagic manifestations of the skin lesions. Conclusion: RCCEP is a characteristic cutaneous adverse reaction of camrelizumab; patients with concomitant diabetes present with more severe skin lesions. Localised lesions are best managed with minimally invasive topical treatment. Individualised local treatment plans developed under a tiered approach are in line with the recommendations of domestic guidelines. RCCEP indicates anti-tumour immune benefit; treatment need not be discontinued in the absence of severe infection.
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Synchronous Primary Malignancies of the Thyroid and Colon: A Case Report
Abstract:
Objective: To investigate the diagnostic and therapeutic characteristics of synchronous quadruple primary malignancies.Methods: The clinical data of a 46-year-old man with synchronous quadruple primary malignancies were retrospectively analyzed, together with the findings of pathological examination, immunohistochemistry, and genetic testing.Results: The patient was diagnosed with papillary thyroid carcinoma of the left lobe, mucinous adenocarcinoma of the ileocecal region, and moderately to poorly differentiated adenocarcinomas of the transverse and ascending colon. Immunohistochemistry showed loss of MLH1/PMS2 protein expression in the colorectal cancer tissues, and genetic testing identified a pathogenic germline variant in MLH1, confirming Lynch syndrome. The patient underwent synchronous radical resection of the thyroid and colorectal tumors, followed by adjuvant oxaliplatin-based chemotherapy. No obvious evidence of recurrence or metastasis was observed during follow-up.Conclusion: Synchronous quadruple primary malignancies are rare. In patients presenting with multiple primary tumors and a relevant family history, pathological and molecular genetic testing should be considered to establish an accurate diagnosis and guide individualized treatment and cascade screening of at-risk family members.
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A CBCT Study on Adult Mandibular Morphology of Skeletal Class Ⅱ Malocclusion with Mandibular Deviation
Abstract:
Objective: To characterize the three-dimensional mandibular morphology of adults with skeletal Class II malocclusion and mandibular deviation using cone-beam computed tomography (CBCT), identify independent morphologic indicators, and construct a combined discrimination model. Methods: A total of 126 adults with skeletal Class II malocclusion and mandibular deviation were retrospectively included as the deviation group, and 118 adults with normal occlusion were selected as the normal group. Three-dimensional mandibular reconstruction and measurements were performed using Mimics 20.0. Paired and independent-samples t tests were used for group comparisons. Variables showing between-group differences were entered into univariable logistic regression; multivariable logistic regression was then performed after correlation and variance inflation factor assessment. Receiver operating characteristic (ROC) curves were used to determine the area under the curve (AUC), optimal cut-off, sensitivity, and specificity. Internal validation was performed with 1,000 bootstrap resamples. Results: No significant left-right differences were observed in the normal group (all P>0.05). On the deviated side, condylar length, mandibular body unit length, condylar width, ramus height, mandibular body length, and three volume measurements were lower than those on the contralateral side (all P<0.001). Compared with the normal group, nine morphologic variables were lower in the deviation group, whereas mandibular angle length and chin length did not differ significantly. Condylar length, mandibular body unit length, condylar width, and ramus height remained independently associated with skeletal Class II malocclusion with mandibular deviation in the multivariable model. The combined model yielded an AUC of 0.925 (95% CI, 0.893-0.958), with an optimal probability cut-off of 0.46, sensitivity of 88.10%, and specificity of 84.75%. The bootstrap-corrected AUC was 0.920. Conclusion: Mandibular asymmetry in adults with skeletal Class II malocclusion and mandibular deviation is mainly concentrated in the condyle, ramus, and part of the mandibular body. A combined model based on condylar length, mandibular body unit length, condylar width, and ramus height provides useful quantitative support for three-dimensional CBCT assessment.
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Clinical Significance of Bedside COHb Detection in Early Diagnosis and Intervention of Hemolytic Disease of the Fetus and Newborn
Abstract:
Objective: This study aimed to explore the clinical value of carboxyhemoglobin (COHb) measured by multi-wavelength CO-oximetry for the early auxiliary diagnosis of hemolytic disease of the fetus and newborn (HDFN) and prediction of the selection of clinical intervention strategies. Methods: A total of 310 neonates with hyperbilirubinemia were enrolled in this retrospective study. According to blood group incompatibility and clinical diagnosis, the subjects were divided into two groups: the hemolysis group, consisting of neonates with blood group incompatibility and clinically diagnosed HDFN, which was further subdivided into two subgroups: confirmed subgroup (113 cases, positive direct Coombs test (DAT) and/or antibody release test) and suspected subgroup (53 cases, only positive serum free antibody test); the control group included 144 neonates with negative results of three hemolysis tests and clinically excluded HDFN. Clinical data including birth weight, gestational age and age at diagnosis were collected. Laboratory indicators including COHb, hemoglobin (Hb), percentage of reticulocytes (Ret%), total serum bilirubin (TBIL) and results of three hemolysis tests were detected. We analyzed the predictive value of COHb for HDFN and its efficacy in evaluating the severity of hemolysis. Results: There were statistically significant differences in age at admission, gestational age and feeding patterns between the hemolysis group and the control group (P<0.05), while no significant differences were observed in birth weight and gender. Significant differences were found in admission TBIL, Hb, COHb and Ret% between the two groups (P<0.05). The median COHb level in the hemolysis group was higher than that in the control group (1.5% vs 1.2%, P<0.01). ABO-HDFN accounted for 96.39% of the hemolysis group. The COHb level of the confirmed subgroup was higher than that of the suspected subgroup (P<0.05). Higher COHb levels were correlated with more frequent phototherapy, higher rates of exchange transfusion and intravenous immunoglobulin (IVIG) use. The COHb cut-off value of 1.45% yielded a sensitivity of 70.48% and a specificity of 86.84% for diagnosing HDFN. Combined detection of COHb, Ret% and Hb improved the diagnostic sensitivity (74.43%) and specificity (87.61%). Conclusion: The COHb level was significantly higher in the hemolysis group than in the control group, and higher in the confirmed subgroup than in the suspected subgroup. Higher COHb levels were associated with greater requirements for clinical interventions. COHb can serve as a reference indicator for auxiliary diagnosis and severity assessment of HDFN.
临床医学研究
护理学
临床医学研究
基础研究
临床医学研究
基础医学研究
临床研究
基础医学研究
短篇与个案
临床医学研究
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Development and validation of a malignancy risk prediction model for pul-monary nodules based on multivariable analysis and machine learning
Hu Xin; Jiang Yongjie; Shilun Guang; Heibieluo; Huang Yuyan; Jiang Li;
2025,40(6):686-692 712 ,DOI: 10.3969/j.issn.1005-3697.2025.06.002
Abstract:
Objective:To develop a robust and widely applicable predictive model to improve the accuracy of diagnosing the ma-lignancy risk of pulmonary nodules.Methods:This study retrospectively collected clinical data from 1,414 patients with pulmonary nod-ules diagnosed and treated at the Affiliated Hospital of North Sichuan Medical College and the Guang'an People's Hospital.Meta-analy-sis and Least Absolute Shrinkage and Selection Operator(LASSO)regression were used to identify predictors related to the malignancy risk of pulmonary nodules.These factors were further optimized by multivariable Logistic regression(LR)to determine key features.Based on these features,8 machine learning models were constructed and evaluated for performance using Receiver Operating Character-istic(ROC)curves,calibration curves,and Decision Curve Analysis(DCA)in the training set and internal validation set.The best-performing model was used to develop a nomogram for risk stratification of patients.Results:Through the combined screening process of Meta-analysis,LASSO regression,and multivariable LR,10 key predictive factors were identified and integrated into eight different ma-chine learning models.Model evaluation demonstrated that the LR model performed best,achieving an Area Under the Curve(AUC)of 0.843 in the internal validation cohort.Additionally,the nomogram derived from this model exhibited strong predictive ability in the ex-ternal validation cohort,with an AUC of 0.770.Risk scores calculated from the nomogram stratified patients into four risk groups,with malignancy rates ranging from 0%in the low-risk group to 100%in the very high-risk group.Conclusion:The prediction model devel-oped in this study effectively assesses the malignancy risk of pulmonary nodules,providing a valuable risk stratification tool for clinical use.
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DOK1 recombinant adenovirus enhances insulin sensitivity of BRL-3A cells through DOK1/AKT/SREBP1c signalling pathway
Cai Xiaoling; Wang Chunlan; Ha Xiaoqin;
2025,40(6):681-685 ,DOI: 10.3969/j.issn.1005-3697.2025.06.001
Abstract:
Objective:To investigate the mechanism of DOK1 recombinant adenovirus(Ad-DOK1)on the regulation of insulin sensitivity in palmitic acid(PA)-induced insulin-resistant hepatocytes(BRL-3A).Methods:The control,solvent and model groups were treated with normal medium,ethanol and palmitic acid to establish a cellular insulin resistance model of BRL-3A cells and to iden-tify the cellular model using cellular glucose uptake assay.The control,null and adenovirus DOK1 overexpression groups were transfect-ed with normal serum,negative virus and Ad-DOK1(51257-1)in insulin-resistant BRL-3A cells,and the transfection efficiency of ade-novirus was detected by flow cytometry,and the Western blot method was used to detect DOK1,IRSI,IRSII,p-PI3K,PI3K,p-AKT,AKT,SREBP1c protein expression levels.Results:Palmitic acid induction for 6 hours resulted in a decrease in intracellular 2-deoxyglu-cose(P＜0.05),down-regulation of DOK1 protein expression level(P＜0.05),no significant change in IRSI and IRSII protein ex-pression level(P>0.05),no change in p-PI3K/PI3K ratio(P>0.05),down-regulation of p-AKT/AKT ratio(P＜0.05),and SREBP1c protein expression expression levels were upregulated(P＜0.05).After transfection of cells with DOK1 recombinant adeno-virus of optimal MOI 60,compared with the normal control group,intracellular 2-deoxyglucose was elevated(P＜0.05),DOK1 protein expression level was up-regulated(P＜0.05),there was no significant change in the expression level of IRSI and IRSII proteins(P>0.05),no significant change in the p-PI3K/PI3K ratio(P>0.05),the p-AKT/AKT ratio was upregulated(P＜0.05),and the ex-pression level of SREBP1c protein expression was downregulated(P＜0.05).Conclusion:Ad-DOK1 may enhance insulin sensitivity of BRL-3A cells by regulating AKT and SREBP1c.
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Effect and mechanism of miR-139-3p targeting SPP1 on cisplatin resist-ance in A549 lung cancer cells
2025,40(10):1225-1229 1248 ,DOI: 10.3969/j.issn.1005-3697.2025.10.001
Abstract:
Objective:To explore the effect and mechanism of miR-139-3p targeting secreted phosphoprotein 1(SPP1)on cis-diamminedichloroplatinum(DDP)in A549 lung cancer cells.Methods:A cisplatin(DDP)-resistant A549/DDP cell model was established via gradient concentration induction,validated by resistance index(IR=3.877)and IC50 determination.The re-sistant cells were divided into five groups:blank control,negative control,miR-139-3p mimic,SPP1 overexpression,and SPP1 knockdown.Multi-platform analyses were performed,including qPCR(miR-139-3p and SPP1 expression),Western blot(SPP1,p-AKT,and total AKT protein levels),CCK-8(proliferation and drug sensitivity),flow cytometry(apoptosis),and du-al-luciferase reporter assays(miR-139-3p/SPP1 interaction).Results:The cisplatin-resistant A549/DDP cells exhibited a signifi-cantly higher IC50 value compared to parental A549 cells(P<0.05),with a resistance index(IR)of 3.877.Quantitative PCR(qPCR)analysis revealed that miR-139-3p expression was markedly upregulated in the miR-139-3p mimic group versus the negative control group(P<0.05).Relative to the miR-139-3p mimic group,SPP1 mRNA levels were significantly elevated in the SPP1-overex-pression group and reduced in the SPP1-knockdown group(P<0.05).Western blotting confirmed corresponding changes in SPP1 pro-tein expression across these groups(P<0.05).CCK-8 assays demonstrated that miR-139-3p overexpression enhanced the proliferation inhibition rate and reduced the IC50 value compared to the negative control(P<0.05),whereas SPP1 overexpression reversed these effects,lowering the inhibition rate and increasing IC50(P<0.05).Compared with the miR-139-3p blank control,the luciferase activity of the wild-type SPP13'UTR luciferase reporter plasmid binding to miR-139-3p analog decreased(P<0.05),and the difference in the luciferase activity of the mutant SPP13'UTR luciferase reporter plasmid binding to miR-139-3p analog was not statistically significant(P>0.05).Compared with the negative control group,the protein expression level of p-AKT in miR-139-3p control group was signifi-cantly decreased(P<0.05),compared with the miR-139-3p control group and SPP1 knockdown group,the protein expression level of p-AKT in SPP1 overexpression group was significantly increased(P<0.05).Conclusion:Overexpression of miR-139-3p or knockdown of SPP1 could inhibit the proliferation of A549/DDP cells,enhance their sensitivity to cisplatin,and the mechanism may be related to the AKT signaling pathway.
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Value of lung ultrasound score combined with peripheral blood procalci-tonin,C-reactive protein and interleukin-6 levels on evaluating the disease severity and prognosis of patients with acute respiratory distress syn-drome
Wang Xiyu; Liao Rui; Wang Zhong;
2025,40(10):1244-1248 ,DOI: 10.3969/j.issn.1005-3697.2025.10.005
Abstract:
Objective:To explore the value of lung ultrasound(LUS)score combined with peripheral blood procalcitonin(PCT),C-reactive protein(CRP)and interleukin-6(IL-6)levels on evaluation of disease severity and prognosis of patients with acute respiratory distress syndrome(ARDS).Methods:109 patients with ARDS were selected.The disease severity and progno-sis of patients were grouped according to the Berlin criteria for ARDS.The baseline data were compared among mild group(n=24),moderate group(n=55)and severe group(n=30).According to the prognosis,they were divided into a survival group(n=68)and a death group(n=41).The differences in LUS score,peripheral blood PCT,CRP and IL-6 levels among the three groups and patients with different prognoses were analyzed,and the evaluation value of the above indexes on the disease severity and prognosis was analyzed.Results:There were statistical differences in prognosis and APACHE Ⅱ score among the three groups(P<0.05).The survival rate of patients in severe group was lower than that in moderate group(P<0.05),and the rate in moderate group was lower than that in mild group(P<0.05).The APACHE Ⅱ score of patients in severe group was higher than that in moderate group(P<0.05),and the score in moderate group was higher than that in mild group(P<0.05).The LUS score and peripheral blood PCT,CRP and IL-6 levels revealed statistical differences among ARDS patients with different disease severities(P<0.05).The severe group was higher than the moderate group(P<0.05),and the moder-ate group was higher than the mild group(P<0.05).Spearman analysis showed that LUS score and peripheral blood PCT,CRP and IL-6 in ARDS patients were positively correlated with disease severity(P<0.05).The LUS score,PCT,CRP and IL-6 in death group were higher than those in survival group(P<0.05).After Logistic regression analysis,it was found that A-PACHE Ⅱ score,LUS score,PCT,CRP,IL-6 and disease severity were the influencing factors of prognosis in patients with ARDS(P<0.05).ROC curve results showed that the areas under the ROC curves of LUS score,peripheral blood PCT,CRP and IL-6 and combined detection for predicting the death prognosis in ARDS patients were 0.778,0.781,0.864,0.856 and 0.982,and the sensitivities were 75.61％,78.05％,90.24％,80.49％and 92.68％,respectively.The combined detection value was higher.Conclusion:LUS score and peripheral blood PCT,CRP and IL-6 levels can effectively evaluate the disease severity of ARDS patients,and have reliable predictive value for prognosis of patients.
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Clinical study of Nalbuphine as local anesthetic adjuvant for nerve block analgesia in elderly patients after total hip arthroplasty
Wang Huajuan; Liu Qiyu; Liu Jing; Li Mingming; Song Man; Li Fengjuan; Zheng Xiaozhen;
2025,40(10):1253-1258 ,DOI: 10.3969/j.issn.1005-3697.2025.10.007
Abstract:
Objective:To observe the effect of ultrasound-guided suprainguinal fascia iliaca compartment block(U-SFICB)with ropivacaine combined with different doses of nalbuphine on analgesia in elderly patients after total hip arthroplasty(THA).Methods:101 elderly patients with unilateral THA under general anesthesia were divided into four groups according to different anesthesia regimens:100 mg ropivacaine+normal saline group(R group,n=26),100 mg ropivacaine+10 mg nal-buphine group(LN group,n=25),and 100 mg ropivacaine+15 mg nalbuphine group(MN group,n=25),and 100 mg ropiv-acaine+20mg nalbuphine group(HN group,n=25),all were diluted to 30 mL with 0.9%sodium chloride injection.All four groups underwent U-SFICB in the post-anesthesia care unit(PACU),and sufentanil was used for patient-controlled intravenous analgesia after surgery.Comparison between groups of postoperative 4,8,12,24,and 48 h of resting and motion state of Numer-ical rating scale(NRS).Each group received 12 h preoperatively,12 and 24 h postoperatively,Interleukin 6(IL-6),Tumor nec-rosis factor-α(TNF-α),Interleukin 10(IL-10)concentration,the first start time of postoperative analgesia pump and 48 h of sufentanil use in each group,the early postoperative recovery of each group,and the occurrence of analgesia related adverse reac-tions 48 h after surgery were compared between groups.Results:Compared with R group and LN group,NRS score in MN and HN group was decreased at all time points(P<0.05),IL-6 and TNF-α concentrations were decreased at all time points(P<0.05),IL-10 concentrations were increased at all time points(P<0.05),and the start-up time of postoperative analgesia pump was significantly extended(P<0.05).The amount of sufentanil was significantly decreased 48 hours after operation(P<0.05).Compared with R group,LN group and HN group,the time of first anal exhaust and getting out of bed in MN group were significantly shortened(P<0.05).The incidence of adverse reactions was significantly decreased 48 h after operation(P<0.05).Compared with HN group,the start-up time of postoperative analgesic pump in MN group was significantly short-ened(P<0.05),and the overall incidence of adverse reactions was the lowest at 48 h after surgery(P<0.05).Conclusion:15 mg nalbuphine combined with ropivacaine can significantly prolong the duration of U-SFICB analgesia after THA in elderly pa-tients,and the first postoperative anal exhaust time and the first time of getting out of bed activity are the shortest,and the inci-dence of adverse reactions is the lowest within 48 hours after surgery,which can be clinically promoted and applied.
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Distribution of peripheral blood B cell subsets in patients with systemic lupus erythematosus and their clinical applications
2025,40(10):1285-1290 ,DOI: 10.3969/j.issn.1005-3697.2025.10.014
Abstract:
Objective:To analyze the distribution of peripheral blood B cell subsets in patients with systemic lupus erythe-matosus(SLE)and their clinical applications.Methods:52 SLE patients(SLE group),36 healthy controls(HC group)and 30 rheumatoid arthritis(RA group)patients were included.The percentage of B1,FOB,Breg and ABC cells in total B cells was measured by Flow cytometry.Diagnostic efficacy of individual and combined B cell subsets for SLE was evaluated via ROC curves.Clinical data on SLE activity score(SLEDAI),erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),com-plement C3 and C4,immunoglobulin,and various autoantibodies from SLE patients were collected,and the correlations between the subsets percentage and these indicators were analyzed.Results:Compared to HC group,the percentage of B1,Breg,and ABC were higher in SLE group(P<0.05)and the percentage of FOB was lower(P<0.05).The percentage of B1,and Breg in SLE group were obviosly higher than those in RA group(P<0.05)and FOB was lower(P<0.05).There was no statistically sig-nificant difference in the proportion of cells in each subgroup between the RA group and the HC group(P>0.05).ROC curve analysis showed that all B cell subgroups had a certain diagnostic efficacy for SLE,and the combination of multiple subgroups had a higher diagnostic efficacy for SLE.The area under the curve(AUC)of 11 combinations for diagnosing SLE was 0.756～0.892(P<0.05),with(ABC+B1+Breg+FOB)combined was highest.Results of Assay of correlations showed that the per-centage of ABC cells positively correlated with the level of ESR(P<0.05,r=0.403).The percentage of Breg cells negatively correlated with the level of C3(P<0.05,r=-0.421)and positively correlated with the level of anti-dsDNA(P<0.05,r=0.488),anti-M2(P<0.05,r=0.604),SLEDAI(P<0.05,r=0.353).ABC and FOB were negatively correlated in the SLE group(P<0.05,r=-0.571),while there was no correlation between the two in the HC and RA groups(P>0.05).Conclu-sion:There are differences in the distribution of B cell subsets between SLE patients and the normal population.The combina-tion of single or multiple indicators has good diagnostic efficacy for SLE,and the distribution of subsets is correlated with multi-ple clinical and laboratory indicators,suggesting that it may have value in disease diagnosis and disease assessment.
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Primary study of vortioxetine in inhibiting proliferation and migration of esophageal cancer cells
Roman; Ma Qiang; Zhong Xiaowu; Liao Zhongbo; Xu Lei; Guo Xiaolan;
2025,40(5):545-550 ,DOI: 10.3969/j.issn.1005-3697.2025.05.001
Abstract:
Objective:To investigate the effects and mechanisms of vortioxetine on the proliferation and migration of esophageal squamous cell cancer.Methods:CCK-8 assay was used to detect the viability of Eca109 and KYSE150 cells after treated with vortioxe-tine at different concentrations(0,2,4,6,8,10 μmol/L).Moreover,transwell and scratch assays were employed to evaluate the migra-tion of ESCC cells,plate colony formation assay was used to detect the proliferation.The differentially expressed genes were screened by TCGA database,AutoDock software was used to predict the binding of vortioxetine and cyclooxygenase-2(COX-2)protein.Further-more,the expression of COX-2 mRNA and protein were measured by RT-qPCR and Western blot,respectively.Results:The cell viabil-ity of Eca109 and KYSE150 decreased in a concentration-dependent manner after treatment with different concentrations of vortioxetine(P<0.05),and the semi-inhibitory concentrations(IC50)were 5.086 μmol/L and 5.179 μmol/L,respectively.Compared with the control group,the cell proliferation and migration of the vortioxetine group were reduced(P<0.05).In addition,vortioxetine could in-hibit the expression of COX-2 at mRNA and protein level(P<0.05).Conclusion:Vortioxetine inhibits the proliferation and migration of esophageal cancer cells probablly through COX-2 down-regulation.
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The causal relationship between sleep disorders and gastric ulcer incidence based on two-sample Mendelian randomization
2025,40(6):693-698 ,DOI: 10.3969/j.issn.1005-3697.2025.06.003
Abstract:
Objective:To investigate whether there was a potential two-way causal relationship between sleep disorder(SD)and the incidence of gastric ulcer(GU)by two sample Mendelian randomization(MR)analysis.Methods:The data sets of exposure and outcome were obtained from IEU database,from which suitable Single Nucleotide Poly Morphisms(SNPs)were selected as instrumental variables(IVs),and then inverse variance weighting(IVW),MR-Egger regression,weighted median(WME),simple model and weigh-ted model were used for two-way MR Analysis.Cochran's Q test and MR-Egger intercept test were used for sensitivity analysis.Results:The IVW method showed that there was a significant unidirectional causal relationship between SD and the incidence of GU.When SD were used as exposure factors,the positive MR Analysis had significant statistical significance(OR=1.009,95%CI:1.004～1.015,P=0.001).When GU used as an exposure factor,reverse MR Analysis was not statistically s-ignificant(OR=1.197,95%CI:0.425～3.371,P=0.734).Further sensitivity analysis showed no significant heterogeneity and horizontal pleiotropy.Conclusion:Mendelian randomization analysis found that there is a potential one-way causal relationship between SD and the risk of GU,and SD are risk factors for GU.
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Application value of contrast-enhanced CT histogram parameters in mor-tality risk assessment of severe acute pancreatitis
Taodi; Zhao Zisheng; Chen Junhui; Zhang Xiaoming;
2025,40(5):562-568 ,DOI: 10.3969/j.issn.1005-3697.2025.05.004
Abstract:
Objective:To optimize contrast-enhanced computed tomography(CECT)image quality using Non-Local Means(NLM)denoising technology and construct a mortality risk prediction model for severe acute pancreatitis(SAP)patients based on his-togram parameters.Methods:Retrospective analysis of clinical,laboratory,and CECT data of 74 patients diagnosed with SAP.NLM de-noising was applied to arterial and portal venous phase images at different coefficient levels.Three-dimensional volumes of interest were manually delineated in pancreatic parenchyma on original and denoised images,followed by histogram parameter extraction.After univa-riate Logistic regression feature selection,binary Logistic regression models were built to predict SAP mortality risk and compared with clinical models and conventional CT models.Results:A total of 74 patients with SAP,of whom 27(36.5%)died.After applying NLM-based noise reduction to arterial phase and portal venous phase CECT images,8 histogram prediction models were constructed under dif-ferent noise coefficient levels.There was no significant difference in predictive performance among these histogram models(P＞0.05).Compared to conventional CT models and clinical models,the histogram models demonstrated higher accuracy in predicting mortality risk(P=0.009,P=0.048).The predictive performance of the clinical model and the conventional CT model was similar(P=0.699).Conclusion:CECT histogram parameters show significant clinical value in predicting mortality risk in SAP patients,maintaining stable performance under different noise coefficient levels after NLM denoising.Their noise robustness and quantitative a-nalysis advantages compensate for the limitations of conventional CT.
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Relevant studies that NETs promote the secretion of inflammatory media-tors by alveolar-type epithelial cells
Xu Suqing; Tian Jiamei; Lu Hongyan;
2025,40(5):557-561 ,DOI: 10.3969/j.issn.1005-3697.2025.05.003
Abstract:
Objective:To explore the role of neutrophil extracellular traps(NETs)in promoting the secretion of inflammatory mediators by alveolar type epithelial cells(AECII).Methods:In 30 7 d born SD rats,blood was drawn from neutrophils(NE)and di-vided into control(control group),LPS and LPS+DNase groups according to different treatment methods.NETs formation was detec-ted by immunofluorescence in vitro.NETs and AECII were co-cultured,and divided into control group(control group),NETs group and NETs+DNase group according to cell suspension incubation method.AECII apoptosis was detected by flow method,the content of free deoxyribonucleic acid(cf-DNA)in coculture supernatants in each group was detected by fluorescence microarray,and the coculture su-pernatants of interleukin 6(IL-6),interleukin 8(IL-8)and tumor necrosis factor(TNF-α)in each group were determined using ELISA kit.And,the correlation between cf-DNA and IL-6,IL-8,and TNF-α was evaluated using Pearson correlation analysis.Results:According to immunofluorescence,mouse citrullinated histone H3(CitH3)and myeloperoxidase(MPO)were found in neutrophil in LPS and LPS+DNase group,CitH3 and MPO levels in LPS and LPS+DNase groups were higher than in the control group(P<0.05),and CitH3 and MPO in LPS+DNase group were lower than those in LPS group(P<0.05).After AECII apoptosis with NE and NETs,there was no significant difference between early and late apoptosis in the three groups(P＞0.05).The levels of cf-DNA,IL-6,IL-8,and TNF-α in NETs group and NETs+DNase group were higher than those of the control group(P<0.05),NETs+DNase group was lower than NETs group(P<0.05).The content of cf-DNA in cell culture supernatant was positively correlated with the levels of IL-6,IL-8,and TNF-α(r=0.432,0.387,0.326,P<0.05).Conclusion:AECII with NETs in the supernatants with NE,NETs could promote the secretion of inflammatory mediators such as IL-6,IL-8,and TNF-α.Moreover,the more NETs are generated,the more inflammatory mediators such as IL-6,IL-8,and TNF-α are secreted.
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Correlation between serum brain-derived neurotrophic factor and mono-cyte to HDL-C ratio levels and post-stroke cognitive impairment in pa-tients with acute ischemic stroke
Shen Yongling; Fang Jianwei; Cheng Fei; Sang Xiaoyi; Meng Jun; Ma Cuihong; Gohong Country;
2025,40(5):624-627 ,DOI: 10.3969/j.issn.1005-3697.2025.05.018
Abstract:
Objective:To investigate the correlation between serum brain-derived neurotrophic factor(BDNF),monocyte to HDL-C ratio(MHR)and post-stroke cognitive impairment(PSCI)in patients with acute ischemic stroke(AIS).Methods:96 patients with acute AIS were collected and divided into PSCI group(n=39,<26 points)and non-PSCI group(n=57,≥26 points)accord-ing to the Montreal Cognitive Assessment(MoCA)score at 3 months after stroke.The clinical data such as serum BDNF and MHR lev-els at admission were statistically compared between the two groups,and the receiver operating characteristic(ROC)curve of BDNF and MHR levels in predicting PSCI was drawn.Results:Compared with the non-PSCI group,the PSCI group was older,had shorter years of education,had a higher proportion of hypertension,and had lower serum BDNF and higher MHR levels,the differences were signifi-cant(P<0.05).Logistic regression analysis showed that age(OR=1.530),hypertension(OR=1.335),serum BDNF level(OR=0.755)and MHR(OR=1.645)were risk factors for PSCI in AIS patients(P<0.05).ROC curve analysis showed that the AUC of BDNF and MHR levels in predicting PSCI were 0.833 and 0.745,respectively,and the AUC of the combined diagnosis of the two was 0.882,which was greater than that of a single index(P<0.05).Conclusion:Serum BDNF and MHR are closely related to PSCI in AIS patients.Both indicators can predict the occurrence of PSCI,and the combined detection of the two can improve the predictive abili-ty.
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Effect of evolocumab in patients with early neurological deterioration after noncardiac acute ischemic stroke
Wang Xiaogan; Fan Ying; Fan Lijuan; Zhou Lifei; Gao Yongchao; Liu Minxiao;
2025,40(5):593-596 ,DOI: 10.3969/j.issn.1005-3697.2025.05.011
Abstract:
Objective:To examine the impact of the Evolocumab on early neurological deterioration(END)in patients with non-cardioembolic acute ischemic stroke(NCIS).Methods:A total of 110 patients diagnosed with NCIS and END were recruited as re-search subjects.All patients received standardized and systematic treatment protocols.Based on different therapeutic regimens,the pa-tients were categorized into two groups:the conventional group(n=55),which received standard dual antiplatelet therapy,and the ob-servation group(n=55),which received evolocumab in addition to the standard treatment regimen.The clinical efficacy,cerebral blood flow perfusion indicators[resistance index(RI),cerebral blood volume(CBV),and mean blood flow(Qmean)],levels of nerve func-tion reconstruction factors[nerve growth factor(NGF),gamma aminobutyric acid(GABA),and brain-derived neurotrophic factor(BDNF)],lipid metabolism indicators[apolipoprotein B(ApoB),low-density lipoprotein cholesterol(LDL-C)],and incidence of ad-verse reactions were compared between the two groups.Results:The total clinical efficacy rate in the observation group was higher than that in the conventional group(98.18%vs.85.45%,P<0.05).Post-treatment,the RI value in the observation group was lower than that in the conventional group(P<0.05).Additionally,post-treatment serum levels of CBV and Qmean,as well as NGF,GABA,and BDNF,were higher in the observation group compared to the conventional group(P<0.05).Conversely,post-treatment serum levels of Lp(a),ApoB,and LDL-C,and the total incidence of adverse reactions were lower in the observation group compared to the conventional group(P<0.05).Conclusion:Evolocumab enhances cerebral blood perfusion and facilitates neurological function recovery in NCIS patients with early END.Additionally,it regulates lipid metabolism and decreases the incidence of adverse reactions.
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Effect of esketamine combined with erector spinae plane block on the qual-ity of recovery in patients after thoracoscopic lobectomy under general an-esthesia
Liu Tongxin; Hou Yongchao; He Peng; Zhang Zhenghui; Zhou Shuzhi;
2025,40(6):708-712 ,DOI: 10.3969/j.issn.1005-3697.2025.06.006
Abstract:
Objective:To investigate the effect of intravenous esketamine combined with erector spinae plane block(ESPB)on the postoperative recovery quality in patients undergoing thoracoscopic lobectomy with general anesthesia.Methods:A total of 97 pa-tients who underwent thoracoscopic lobectomy were divided into two groups:group K(n=49)and group C(n=48),according to whether or not they were given esketamine.Patients in K group were given slow intravenous injection of 0.2 mg/kg ketamine after anes-thesia induction and 5 minutes before surgical skin incision,and patients in group C received intravenous injection of physiological sa-line equivalent to group K drugs.Comparison of general indicators,quality of recovery at 24 h postoperatively[40-item Quality of Re-covery Rating Scale(QoR-40)score],cellular immune function before and after 24 h of treatment(NK cells,CD4+T cells,and CD8+T cells),hemodynamics at each time point,and the occurrence of postoperative adverse events between the two groups.Results:Intrao-perative remifentanil dosage was lower in group K than in group C(P＜0.05).The overall QoR-40 score,as well as the subscores for pain,emotional state,and physical comfort in group K were higher than those in group C at 24 hours postoperatively(P＜0.05).Addi-tionally,levels of NK cells,CD4+T cells,and CD8+T cells were higher in the group K than those in group C at 24 hours postoperative-ly(P＜0.05).There were no statistically significant differences in hemodynamics and total incidence of adverse effects between the two groups(P>0.05).Conclusion:The intravenous application of esketamine combined with ESPB for thoracoscopic lobectomy under general anesthesia reduces intraoperative remifentanil dosage,improves the quality of postoperative recovery and improves postoperative cellular immune function in patients without increasing adverse effects.
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Influence of placental morphology and pathological changes on neonatal outcome in patients with premature rupture of membranes
Gold mineralization; Zhong Hui; Long Luwen; Zhong Wenna;
2025,40(6):787-790 ,DOI: 10.3969/j.issn.1005-3697.2025.06.023
Abstract:
Objective:To study the influence of placental morphology and pathological changes on neonatal outcome in patients with premature rupture of membranes(PROM).Methods:100 parturients with PROM were selected as the study group,and 100 nor-mal parturients without PROM during the same period were selected as the control group.HE staining was used to observe the Placental pathological changes and monitor pregnancy outcome.The Placental pathological changes and pregnancy outcome were compared be-tween the two groups.The influencing factors of premature rupture of membranes were analyzed by Single factor and multiple factor Lo-gistic regression analysis.Results:The incidence of placental pathological changes in the study group was higher(P＜0.05).The inci-dence of adverse pregnancy outcome in the study group was higher than that in the control group(23.00%vs.5.00%,P＜0.05).Uni-variate analysis showed that there were no statistically significant difference in the incidence of uterine malformations,cervical dysfunc-tion,polyhydramnios,anemia during pregnancy,placenta previa,and history of induced labor or miscarriage between the two groups(P>0.05).In the study group,gestational diabetes,hypertension,abnormal fetal position,multiple births,reproductive tract infection and unknown causes were all higher(P＜0.05).Logistic regression analysis showed that the abnormal fetal position,multiple births,and reproductive tract infection were all independent risk factors for premature rupture of membranes(P＜0.05).Conclusion:Prema-ture rupture of membranes is affected by abnormal fetal position,multiple pregnancies,and reproductive tract infections,and the placenta of patients with premature rupture of membranes has pathological changes,which will lead to poor neonatal outcome.
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Analysis of prognostic factors in patients with septic cardiomyopathy and development of prediction model
Li Pengfei; Lin Jiafu; Yuan Shun; Jiang Zhi;
2025,40(6):764-769 ,DOI: 10.3969/j.issn.1005-3697.2025.06.018
Abstract:
Objective:To investigate the predictive factors of prognosis in patients with septic cardiomyopathy and building pre-diction model.Methods:A retrospective study was conducted on 156 patients with purulent pericarditis,and they were divided into a model set and a validation set in a 7∶3 ratio.The patients in the model set were divided into survival and death groups according to dif-ferent outcomes.The influence factors on the prognosis of the model set patients were evaluated by multivariate Cox regression analysis,and a ROC curve and decision curve analysis(DCA)were used to validate the model using R software.Results:Multiple COX regres-sion analysis indicated that lactic acid,D-dimer,interleukin-6,APACHE-Ⅱscore and SOFA score were independent risk factors for 28 deaths in hospital with septic cardiomyopathy.ROC curve analysis results showed that the predictive value curve area(AUC)of the model set was 0.90,and the AUC of the validation set was 0.83,indicated that the model has good value in the prognosis of patients with septic cardiomyopathy.The results of the clinical decision curve(DCA curve)suggested that the clinical benefit of the validation set and the model set in this model was superior to the"assume all patients die"(all)and"assume all patients die"(none)curves.Conclusion:The prediction model has a good fit to the actual and correction curves,approaching the ideal curve,and has certain clini-cal practical value for patients with septic cardiomyopathy.
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Curative effect of Zixiandan combined with dipyridamole and its influences on levels of urine immunoglobulin G and β2 microglobulin in children with Henoch-Schonlein purpura
Zhang Lili; Shang Lili; Cui Heqing;
2025,40(5):573-576 ,DOI: 10.3969/j.issn.1005-3697.2025.05.006
Abstract:
Objective:To explore the curative effect of Zixiandan combined with dipyridamole and its influences on levels of u-rine immunoglobulin G and β2 microglobulin in children with Henoch-Schonlein purpura.Methods:According to different treatment methods,80 children with Henoch-Schonlein purpura were divided into control group(n=40,routine comprehensive treatment+dipy-ridamole)and observation group(n=40,Zixiandan on basis of control group),the course of treatment was 7～14 days.Clinical cura-tive effect,scores of TCM syndromes,recovery time of symptoms,renal function and adverse reactions were compared between the two groups.Results:The effective rate of treatment was higher in observation group than in control group(95.00%vs.72.50%,P<0.05).After treatment,scores of TCM syndromes were lower in observation group than control group(P<0.05),disappearance time of lbuminuria,remission time of purpura,length of hospital stay,disappearance time of joint symptoms and remission time of ab-dominal pain were shorter in observation group(P<0.05),levels of urine β2-MG and IgG were lower in observation group(P<0.05).There was no statistically significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion:Zixiandan combined with dipyridamole can improve TCM symptoms,reduce renal injury and promote recovery process in children with Henoch-Schonlein purpura,which has significant curative effect.
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Efficacy of recombinant human interferon α-1b combined with budesonide aerosol inhalation in treating acute bronchitis in children
Wei Qiong; Rao Rui; Xu Wenyi; Yang Luling; Lu Zhili; Li Zhixin;
2025,40(6):774-777 ,DOI: 10.3969/j.issn.1005-3697.2025.06.020
Abstract:
Objective:To explore the clinical efficacy of recombinant human interferon α-1b combined with budesonide and sin-gle budesonide aerosol inhalation in the treatment of acute bronchitis in children.Methods:106 children with acute bronchitis were se-lected as study samples,and were divided into control group and study group by adopting different treatment methods,with 53 cases in each group.The children in the control group were treated with budesonide aerosol inhalation inhalation in addition to conventional treatment,while the children in the study group were combined with budesonide and recombinant human interferon α-1b aerosol inhala-tion inhalation on the basis of conventional treatment,both groups were treated for 1 week.The regression times of clinical symptoms,changes in serum inflammatory factors levels[procalcitonin(PCT),serum C-reactive protein(CRP)],blood routine[white blood cell count(WBC),neutrophil percentage(NEU%)]and lung function indexes[maximum expiratory volume in one second(FEV1),FEV1 to forced vital capacity ratio(FEV1/FVC),and maximum expiratory flow(PEF)]before treatment and after 1 week of treatment and adverse reactions during treatment were compared.Results:The regression times of clinical symptoms in study group were shorter than those in control group(P＜0.05).After 1 week of treatment,the levels of serum PCT,CRP,WBC and NEU%were lower in study group than those in control group(P＜0.05).The FEV1/FVC,PEF and FEV1 in study group were higher compared with those in con-trol group(P＜0.05).During treatment,there were no statistical differences in the incidence rates of adverse reactions between both groups(P>0.05).Conclusion:Recombinant human interferon α-1b combined with budesonide aerosol inhalation inhalation for chil-dren with acute bronchitis can quickly relieve the clinical symptoms,and improve the inflammation level,and it has high safety.
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Effects of iTBS and 1 Hz rTMS on depressive symptoms and cognitive function in elderly patients with depression
Zhou Xueying; Xu Deyi; Shang Fangjie;
2025,40(6):703-707 ,DOI: 10.3969/j.issn.1005-3697.2025.06.005
Abstract:
Objective:To explore the effects of intermittent theta burst magnetic stimulation(iTBS)and 1 Hz repetitive tran-scranial magnetic stimulation(rTMS)on depressive symptoms and cognitive function in elderly patients with depression.Methods:Eld-erly patients with depression were divided into iTBS group(n=30)and 1 Hz rTMS group(n=43)according to the treatment regi-men.On the basis of antidepressant treatment for both groups,the left dorsolateral prefrontal lobe of the iTBS group was given a stimula-tion frequency of 50 Hz from the internal frequency,5 Hz from the inter-frequency and stimulation strength of 110%RMT,with a stimu-lation time of 2 s,an intermission of 8 s,and a repetition of 20 times,with a total of 600 pulses lasting for 200 s.The 1 Hz rTMS group was given a stimulation frequency of 1.0 Hz and a stimulation intensity of 110%RMT in the dorsolateral right prefrontal lobe,and was treated for 20 min per day,with a total of 1,200 pulses per treatment.Patients in both groups were treated 5 times per week at the same fixed time for 4 weeks for a total of 20 treatments.The Hamilton Depression Scale 24(HAMD-24),Geriatric Depression Scale(GDS),Beck Suicidal Ideation Inventory-Chinese Version(BSI-CV),Wisconsin Card Sorting Test,and Part A of the Connection Test were used for assessment before and after treatment,and adverse reactions during treatment were recorded.Results:With the prolongation of treat-ment time,the HAMD-24 and GDS scores of the two groups gradually decreased(P＜0.05),and the HAMD-24 and GDS scores of the iTBS group were lower than those of the 1 Hz rTMS group,and the difference was statistically significant(P＜0.05).After4 weeks of treatment,the number of correct responses of the patients in the iTBS group was higher than that of the 1 Hz rTMS group,and the num-ber of incorrect responses,BSI-CV score suicidal ideation intensity and suicide risk were lower than those of the 1 Hz rTMS group,and the TMT-A time was shorter than that of the 1 Hz rTMS group,with a statistically significant difference between the groups(P＜0.05).During the treatment period,there was no statistically significant difference in the incidence rate of adverse reactions between the two groups(P>0.05).Conclusion:There are significant advantages of iTBS over1 Hz rTMS in treating elderly patients with depression in terms of improving depressive symptoms,reducing suicidal ideation,and improving cognitive performance,and there was no increased treatment safety risk.
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Clinical effect of rigid neuroendoscopy and traditional drilling irrigation and drainage in treating chronic subdural hematoma
Dong Xichao; Wang Fenglu; Zhao Haikang; Rongbo;
2025,40(6):736-739 ,DOI: 10.3969/j.issn.1005-3697.2025.06.012
Abstract:
Objective:To explore the clinical effect of rigid neuroendoscopy and traditional drilling irrigation and drainage in the treatment of chronic subdural hematoma(CSDH).Methods:The clinical data of CSDH patients were retrospectively analyzed.Accord-ing to different treatment methods,they were divided into traditional group(treated with traditional drilling irrigation and drainage)and endoscopic group(treated with rigid neuroendoscopy),48 cases in each group.The differences in perioperative indicators,GCS score,Markwalder grading and NIHSS score were compared between groups,and the complications and recurrence rate in the two groups were counted.Results:The intraoperative blood loss,hospitalization time and immediate postoperative hematoma clearance rate in endoscopic group were better than those in traditional group(P＜0.05),and the total surgical time in traditional group was shorter than that in en-doscopic group(P＜0.05).At 7 days after surgery,the GCS score and Markwalder neurological function grading in the two groups were improved(P＜0.05),but there were no obvious differences between the two groups(P>0.05).Different time points after surgery,the NIHSS score in both groups was reduced compared to before surgery(P＜0.05),and the score was lower in endoscopic group than that in traditional group(P＜0.05).The complications showed no significant differences(P>0.05),and the recurrence rate of hematoma was lower than that in traditional group(P＜0.05).Conclusion:Both rigid neuroendoscopy and traditional drilling irrigation and drainage can effectively treat CSDH,but neuroendoscopy has higher hematoma clearance rate,shorter hospitalization time and lower postoperative recurrence rate,which is worthy of clinical promotion.
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Diagnostic value of multimodal magnetic resonance imaging in grading and Subtype of meningiomas
Ji Xiaoxiao; Lv Hairong; Jia Rongrong; Zhao Feng;
2025,40(6):713-718 ,DOI: 10.3969/j.issn.1005-3697.2025.06.007
Abstract:
Objective:To analyze the diagnostic value of multimodal magnetic resonance imaging(MRI)in grading and Sub-type of meningiomas.Methods:A total of 98 patients with meningiomas were reviewed.They were divided into benign group(n=78)and malignant group(n=20)according to the pathological examination results.All of them underwent conventional MRI scan and mul-timodal MRI examination upon admission.The multimodal MRI parameters[apparent diffusion coefficient(ADC),local cerebral blood flow(rCBF),magnetic sensitive signal intensity(ITSS),N-acetylaspartate(NAA)/choline(Cho),NAA/creatine(Cr),Cho/Cr]of patients with benign and malignant meningiomas,and patients with different subtypes of benign meningiomas were compared.The diag-nostic value of multimodal MRI for meningioma classification and grading were analyzed by receiver operating characteristic(ROC)curves.Results:The ADC,NAA/Cho ratio and NAA/Cr ratio of meningiomas in the malignant group were lower than those in the be-nign group(P＜0.05).The rCBF,ITSS and Cho/Cr ratio were higher than those in the benign group(P＜0.05).The AUC values of three-dimensional arterial spin labeling(3D-ASL),diffusion-weighted imaging(DWI),susceptibility-weighted imaging(SWI)and proton magnetic resonance spectroscopy(1 H-MRS)for grading of meningiomas were 0.723,0.735,0.777 and 0.870.The AUC of com-bined diagnosis was 0.957.There were significant differences in rCBF,ITSS,NAA/Cho ratio,NAA/Cr ratio and Cho/Cr ratio among patients with different subtypes of benign meningiomas(P＜0.05).Conclusion:Multimodal MRI is helpful in clinical grading and Subtype of meningiomas.
Basic Medical Research
Clinical medical research
Basic Medical Research
Clinical medical research
Basic Medical Research
Clinical medical research
Editor in chief：Dai Rongyang
Deputy Editor-in-Chief：Li Zhi
Governed by：The Education Department of Sichuan Province
Sponsored by：North Sichuan Medical College
Phone：0817-2242637
Email：xuebaocby@126.com
ISSN1005-3697
CN51-1254/R
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