Objective:To explore the efficacy of bifidobacterium quadruple viable bacteria combined with micro-feeding in the treatment of feeding intolerance in premature infants and its influence on gastrointestinal function and plasma ghrelin and cholecystoki-nin(CCK)levels.Methods:A total of 120 premature infants with feeding intolerance were included,and were divided into feeding group(n=60,micro-feeding therapy)and viable bacteria group(n=60,bifidobacterium quadruple viable bacteria combined with mi-cro-feeding therapy)by different intervention methods.The clinical efficacy,recovery status(feeding tolerance recovery time,normal body mass recovery time,abdominal distension disappearance time,vomiting disappearance time),gastrointestinal function(motilin,gh-relin,CCK)and liver function[albumin,total bilirubin(TBIL)]before and after treatment and incidence rates of adverse events were compared between the two groups.Results:The total efficacy in viable bacteria group was higher than that in feeding group(96.67％vs.86.67％,P＜0.05).The recovery time of each item was shorter in viable bacteria group than that in feeding group(P＜0.05).Af-ter treatment,the levels of motilin,ghrelin and albumin in the two groups were increased(P＜0.05)while the levels of CCK and TBIL were decreased(P＜0.05).The motilin,ghrelin and albumin levels in viable bacteria group were higher in viable bacteria group(P＜0.05),the levels of CCK and TBIL were lower compared to feeding group(P＜0.05).The total incidence rate of adverse events in via-ble bacteria group was lower than that in feeding group(3.33％vs.15.00％,P＜0.05).Conclusion:Bifidobacterium quadruple via-ble bacteria combined with micro-feeding can effectively treat feeding intolerance in premature infants,improve gastrointestinal function and reduce the incidence rates of adverse events.It is worthy of clinical promotion and application.