Objective:To explore the application effects of tendon incision localization(TI)and anterior medial localization(AM)in anterior cruciate ligament(ACL)reconstruction.Methods:According to different femoral tunnel localization methods,129 patients who underwent elective arthroscopic ACL reconstruction were divided into TI group(tendon incision positioning,n=65)and AM group(anterior medial positioning,n=64).The general surgical condition,angle and length of femoral tunnel,knee function before surgery,1 month and 3 months after surgery,and surgical complications in 3 months after surgery were compared between the two groups.Results:There was no significant difference between the two groups in terms of diameter of the graft,length of hospital stay,the length of femoral tunnel,and the incidence of surgical complications(P＞0.05).The time for tendon retrieval and surgery time in TI group was shorter than that in AM group(P＜0.05).Sagittal and coronal angles were larger than those in AM group(P＜0.05).Com-pared with preoperative scores,Lysholm scores of both groups increased 1 month and 3 months after surgery(P＜0.05).However,there was no significant difference between the groups(P＞0.05).Conclusion:Applying TI localization can shorten the time for tendon re-trieval and surgery time,and obtain a larger femoral tunnel angle.Both localization techniques can achieve satisfactory clinical results within 3 months after surgery,and the incidence rates of surgical complications are comparable.