Objective:To investigate the composition and antibiotic resistance patterns of clinical bacterial isolates from the Affiliated Hospital of North Sichuan Medical College between 2021 and 2024,to provide a scientific basis for the rational use of antimicrobial agents and hospital infection control.Methods:We collected data on clinical bacterial isolates and their antimicrobi-al susceptibility test results.The data were analyzed using the WHONET 5.6 software.Results:A total of 38423 non-duplicate bacterial isolates were identified during the study period,with Gram-positive bacteria accounting for 30.6％and Gram-negative bacteria for 69.4％.The top five specimen types by isolate count were sputum,pus,urine,blood and bronchoalveolar lavage flu-id.The most frequently isolated bacteria were Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,Haemophilus in-fluenzae,and Pseudomonas aeruginosa.The overall detection rates for methicillin-resistant Staphylococcus aureus(MRSA)was 32.0％.Vancomycin resistance was observed in 0.5％of Enterococcus faecalis isolates,with no vancomycin-resistant Entero-coccus faecium detected.The resistance rates to linezolid for Enterococcus faecalis and Enterococcus faecium were 0.5％and 2.8％,respectively.The detection rates for carbapenem-resistant Escherichia coli,Klebsiella pneumoniae,and Enterobacter cloa-cae were 1.4％,7.0％and 5.4％,respectively.The detection rates for carbapenem-resistant Pseudomonas aeruginosa and Acin-etobacter baumannii were 7.4％and 56.6％,respectively.Conclusion:While the composition and resistance rates of the isolated bacteria showed little variation from 2021 to 2024,there was an increase in resistance rates for Enterobacter cloacae to carbapen-ems,Enterococcus faecium to linezolid and Acinetobacter baumannii to minocycline and tigecycline.Continuous surveillance and enhanced management of antimicrobial use and hospital infection control are warranted.