Objective:To investigate the relationship between serum angiopoietin-1(Ang-1)and Clara cell secretory pro-tein-16(CC-16)levels and the condition of neonates with acute respiratory distress syndrome(ARDS)and the predictive value of prognosis.Methods:136 neonates with ARDS were divided into mild group(n=41),moderate group(n=65)and severe group(n=30)according to the severity of the disease.According to the prognosis within 28 days,they were divided into sur-vival group(n=115)and death group(n=21).The differences of serum Ang-1 and CC-16 levels in each group were com-pared.Logistic regression model was used to analyze the influencing factors of 28-day prognosis of ARDS neonates.The receiver operating characteristic(ROC)curve was used to evaluate the clinical value of Ang-1 and CC-16 levels and their combined pre-diction of prognosis.Results:Compared with the survival group,the serum Ang-1 level in the death group was lower(P<0.05),and the CC-16 level was higher(P<0.05).The levels of serum Ang-1 and CC-16 in children with different severity were statistically different(P<0.05).The more severe the ARDS newborn was,the lower the serum Ang-1 level was(P<0.05),and the higher the CC-16 level was(P<0.05).Logistic regression analysis showed that gestational age(OR=0.853),5 min Apgar score(OR=0.802),serum Ang-1(OR=0.702)and CC-16(OR=2.389)were independent influencing factors for 28-day prognosis of ARDS neonates(P<0.05).ROC curve analysis showed that serum Ang-1 and CC-16 levels had certain predictive value for the prognosis of ARDS neonates,with AUC of 0.710 and 0.734,respectively.The AUC of the combination of the two was 0.788,which was higher than that of the single index(P<0.05).Conclusion:Serum Ang-1 and CC-16 are relat-ed to the condition of ARDS neonates and can be used as biomarkers for prognosis prediction.