Objective: To investigate the therapeutic effect of probiotic-assisted basic periodontal therapy in patients with cirrhosis complicated by periodontitis and its regulatory effect on dysregulation of the oral microbiota-gut-liver axis. Methods: 102 patients with liver cirrhosis and periodontitis were enrolled and divided into an observation group and a control group according to different treatment regimens, with 51 patients in each group. Both groups received basic treatment for liver cirrhosis. On this basis, the control group received periodontal treatment, and the observation group received probiotic-assisted basic periodontal treatment. Periodontal status indicators [Plaque Index (PI), Gingival Bleeding Index (GBI), Probing Depth (PD), Clinical Attachment Loss (CAL)], oral flora, intestinal flora, gingival crevicular fluid indicators [Interleukin-6 (IL-6), Interleukin-1β (IL-1β), Tumor Necrosis Factor-α (TNF-α), Matrix Metalloproteinase-9 (MMP-9)], liver function indicators [Total Bilirubin (TBIL), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST)], liver fibrosis indicators [Hyaluronic Acid (HA), Procollagen III (PCⅢ), Collagen IV (Ⅳ-C)], and the occurrence of adverse reactions were compared between the two groups. Results: After treatment, the levels of PI, GBI, PD, and CAL in the observation group were lower than those in the control group (P<0.05). The quantities of Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans in the oral cavity of the observation group after treatment were lower than those in the control group (P<0.05). The quantities of Enterobacter, Enterococcus, Lactobacillus, and Bifidobacterium in the intestinal flora of the observation group after treatment were higher than those in the control group, and the quantity of Candida albicans was lower than that in the control group (P<0.05). The levels of IL-6, IL-1β, TNF-α, and MMP-9 in the gingival crevicular fluid of the observation group after treatment were lower than those in the control group (P<0.05). The levels of serum liver function indicators TBIL, ALT, and AST in the observation group after treatment were lower than those in the control group (P<0.05). There were no statistically significant differences in the serum liver fibrosis indicators HA, PCⅢ, and Ⅳ-C between the two groups after treatment (P>0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: Probiotics as an adjunct to basic periodontal therapy can improve the periodontal condition of patients with cirrhosis and periodontitis, regulate the balance of oral and intestinal flora, reduce the level of inflammatory factors in the gingival crevicular fluid, improve liver function, and exhibit good safety.