Objective: To observe the expression of CD163 and CD105 in colorectal cancer tissues and explore their clinical significance.Methods: 82 pathological specimens of colon cancer and corresponding normal tissues adjacent to the cancer were collected from our hospital following surgical resection. Immunohistochemistry (IHC) was used to detect the expression of CD163 and CD105 in the two tissue types, and the difference in positive high expression rates of CD163 and CD105 was compared. The differences between the different expression patterns of CD163 and CD105 and clinical parameters of patients, as well as the correlation between CD163 and CD105 expression in colorectal cancer tissues, were analyzed.Results: The positive high expression rates of CD163 and CD105 in colorectal cancer tissues were 68.3% and 53.7%, respectively, while those in normal tissues adjacent to the cancer were 51.2% and 0%, respectively, with statistical significance (P0.05). Immunohistochemical quantitative detection confirmed a positive correlation between the expression levels of CD163 and CD105 in colorectal cancer tissues (P<0.05).Conclusion: The high expression of CD163 and CD105 is associated with tumor TNM staging and lymph node metastasis, and their expression in colorectal cancer tissues is positively correlated. This suggests that the combined high expression pattern of CD163 and CD105 is related to the progression of colorectal cancer, and its related molecular mechanisms can be further studied.