Objective: To explore the impact of different Axis-0.25D cylindrical lenses on visual quality in low-myopic eyes.Methods: A cross-sectional study was conducted, enrolling healthy young eyes (374 eyes) with -0.50 to -3.00D myopia and different axis orientations of -0.25 D astigmatism. A repeated-measures design was used, with spherical and sphero-cylindrical corrections applied to obtain refractive prescriptions and best-corrected visual acuity (CDVA, logMAR) for each participant. The optical quality parameters of the two correction methods were measured using a double-pass optical quality analysis system (OQAS), including Objective Scattering Index (OSI), Modulation Transfer Function cutoff (MTF cutoff), Strehl Ratio (SR), and simulated contrast visual acuity-OQAS values (OV). The results were compared within each axis orientation (WTR, ATR, OBL). Results: In 374 participants (374 eyes), except for -0.25D WTR astigmatism, both ATR and OBL astigmatism showed better CDVA with sphero-cylindrical correction (P0.05). For -0.25D ATR astigmatism, sphero-cylindrical correction showed better results in all optical parameters except SR and 9% VA (P<0.05). For -0.25D OBL astigmatism, sphero-cylindrical correction showed better results in all optical parameters except OSI, SR, and 9% VA (P<0.05).Conclusion: Low myopic eyes with -0.25D ATR or OBL astigmatism can benefit from improved optical quality and visual acuity with cylindrical lens correction.