Objective:To investigate the changes of serum vasoactive intestinal peptide(VIP),angiopoietin-2(Ang-2)and nes-fatin-1 in patients with endometriosis(EMS)and the relationship with disease conditions.Methods:A total of 115 patients with EMS confirmed by postoperative pathology after laparoscopic surgery were included in EMS group.According to the different stages of dis-ease,the patients were divided into early group(n=41,stage Ⅰ～Ⅱ)and late group(n=74,stage Ⅲ～Ⅳ).60 healthy women with physical examination were enrolled as healthy group.Baseline data and clinical indicators between EMS group and healthy group and se-rum indicators between early group and late group were compared.Pearson method was used to analyze the correlation between serum indicators and clinical indicators.After 1 year of follow-up,the patients in EMS group were classified into pregnancy group(n=68)and non-pregnancy group(n=47)by means of outcomes,and serum indicators were compared.The clinical value of serum indicators on evaluating prognosis was analyzed by ROC curve.Results:Abortion history and CA125 in EMS group were higher than those in healthy group(P＜0.05),E2,P and FSH were lower than those in healthy group(P＜0.05).The levels of serum indicators were higher in EMS group than those in healthy group(P＜0.05),and were higher in late group than those in early group(P＜0.05).Ser-um indicators were positively correlated with CA125(P＜0.05),and were negatively correlated with E2,P and FSH(P＜0.05).Ser-um indicators levels in non-pregnancy group were higher than those in pregnancy group(P＜0.05).The AUCs of three serum indica-tors were 0.650,0.701 and 0.727 respectively,and the AUC of the combination of the three was 0.798,indicating a higher value of combined diagnosis.Conclusion:Serum VIP,Ang-2 and nesfatin-1 levels are closely related to the pathogenesis and progression of EMS,which can be used as reference indicators for clinical diagnosis,and combined diagnosis has higher value.