Objective:To clarify the differential expression of LINC01006 in Castration-Resistant Prostate Cancer.To prove that LINC01006 mediates the enhanced proliferation and invasiveness of prostate cancer cells.To clarify that LINC01006 is packaged into exosomes and delivered between cells,resulting in enhanced PCa cell proliferation and invasiveness.Methods:Screen the differentially expressed transcriptomes of normal prostate tissue and prostate cancer tissue by analyzing the biological information database.The ex-pression difference of LINC01006 in normal prostate tissue and cancer tissue was verified by RT-qPCR technology.Using the construc-tion of shRNA expression vector plasmid,combined with lentivirus coating technology,LNCaP and 22RV1 cells were transfected.Using the technology of extracting extracellular medium,by adding RNase and changing the permeability of the probe,it was verified that LINC01006 was covered by the membrane structure.PCa cell exosomes were extracted by high-speed ultracentrifugation and verified by Western Blot.The expression difference of LINC01006 in exosomes was verified by RT-qPCR technology.CCK-8 assay and colony for-mation assay were used to verify the changes of PCa cell proliferation and migration ability in the case of LINC01006 knockout.CCK-8 proliferation activity experiment and plate cloning experiment When exosomes derived from homologous and xenogeneic PCa cells were co-cultured,the recovery of PCa cell proliferation and migration ability in the case of LINC01006 knockout.Results:Compared with nor-mal prostate epithelial cells(RWPE-1),the expression of LINC01006 in LNCaP and 22RV1 cells was up-regulated(P＜0.01).The expression of LINC01006 in knockdown LNCaP was down-regulated by 84.7％compared with the control group(P＜0.01),and the expression of LINC01006 in knock-down 22RV1 was down-regated by 80.3％compared with the control group(P＜0.01).There was no significant difference between CM group and CM+RNase group(P＞0.05).Compared with the other two groups,the detection of LINC01006 in the CM+RNase+T group was significantly lower(P＜0.01).The exosomes were successfully extracted by high-speed uLtracentrifugation,and the specific proteins CD63,CD81 and HSP70 were highly expressed(P＜0.05).Exosomal LINC01006 expres-sion derived from LNCaP cells was significantly increased(P＜0.01).The expression of exosome LINC01006 derived from 22RV1 cells was significantly increased(P＜0.01).Compared to the control group(siCtrl),the absorbance of the knockout group(si1006)cells on the 3rd and 4th day was obviously lower than that of the control group(P＜0.01).Compared to the control group(siCtrl),the cells of the knockout group(siCtrl),the number of clones were significantly reduced(P＜0.05).As opposed to the control group(Ctrl),the absorbance of cells in the exosome group(LNCaP exosome)on the 4th day was obviously higher than that in the control group(P＜0.05).As opposed to the control group(Ctrl),the number of cell clones in the exosome group was significantly increased(P＜0.05).Compared with the control group(Ctrl),the absorbance of cells in the exosome group(22RV1 exosome)on the 3rd and 4th day was significantly bigger than which in the control group(P＜0.05).As opposed to the control group(Ctrl),the number of cell clones in the exosome group was significantly increased(P＜0.01).Conclusion:LINC01006 is highly expressed in PCa cell lines and can promote tumor proliferation and migration.LINC01006 is coated with exosomes and secreted into the extracellular space through PCa cells to exert corresponding biological activities.PCa-derived exosomes can induce LINC01006 PCa cells to restore their proliferative ca-pacity.