Objective:This study integrates network pharmacology,molecular docking,and experimental validation to sys-tematically investigate the mechanism of Periplaneta americana extract in treating neuroblastoma(NB).Methods:The core components of Periplaneta americana extract were identified through PubMed and CNKI databases.Potential targets were pre-dicted using the SwissTargetPrediction database,while NB-related targets were analyzed through OMIM,GeneCards,and Dis-GeNET databases,with intersection targets determined using the Weishengxin platform.Protein-protein interaction(PPI)net-works were constructed using the STRING database,followed by GO functional and KEGG pathway enrichment analyses via the DAVID database.Molecular docking validation was performed using AutoDock Tools.The anti-proliferative effects on NB cells were evaluated through CCK-8 and colony formation assays.Apoptosis induction and cell cycle effects were assessed by flow cytometry,with Western blotting employed to detect Bax and Bcl-2 expression levels.Results:17 peptide-based active com-ponents and 323 potential therapeutic targets were identified.Enrichment analysis suggested the extract exerts anti-NB effects through apoptosis-related signaling networks.Molecular docking confirmed stable binding between core components and key targets.CCK-8 assays demonstrated significant inhibition of NB cell proliferation(P＜0.05)without notable cytotoxicity to normal cells.The extract dose-dependently reduced clonogenic capacity and promoted tumor cell apoptosis.Conclusion:Peripla-neta americana extract synergistically inhibits NB cell proliferation and induces apoptosis through multi-target mechanisms and pathway regulation.