Objective:To investigate the expression level of serum microRNA(miR)-101 in the diagnosis of cervical canc-er and its relationship with clinicopathological parameters.Methods:76 patients with cervical cancer(cervical cancer group),50 patients with cervical intraepithelial neoplasia(CIN)(CIN group)and 50 patients with benign uterine lesions(control group)diagnosed were selected.The differences of serum miR-101 expression and general data among the three groups were compared.Logistic regression model was used to analyze the influencing factors of cervical cancer.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficacy of miR-101 for cervical cancer,and its relationship with different clinical path-ological parameters in cervical cancer patients.Results:The relative expression of serum miR-101 in cervical cancer group was(0.48±0.14),which was lower than that in CIN group(0.93±0.17)or control group(1.01±0.19),and the difference was statistically significant(P＜0.05).Logistic regression analysis showed that HR-HPV infection and serum miR-101 level were the influencing factors of cervical cancer(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of serum miR-101 for diagnosing cervical cancer was 0.886(95％CI:0.829～0.929).There was no statistically significant difference in the expression levels of serum miR-101 among cervical cancer patients of different ages,menopausal status,and tumor diameter(P＞0.05),but there was a statistically significant difference in the expression levels among different pathological stages,lymph node metastasis,and vascular infiltration(P＜0.05).ROC curve analysis showed that serum miR-101 had certain diag-nostic value for pathological stage(AUC=0.792),lymph node metastasis(AUC=0.755)and vascular invasion(AUC=0.730)of cervical cancer.Conclusion:miR-101 is lowly expressed in the serum of patients with cervical cancer and is associated with the malignant degree of cervical cancer,which can be used as a screening marker for cervical cancer.