Objective: To explore the clinical efficacy of concentrated growth factor (CGF) filling combined with carbon dioxide (CO2) fractional laser treatment for atrophic scars following subcutaneous separation surgery.Methods: A total of 82 patients with depressed scars were enrolled as study subjects. They were grouped according to treatment modality into a control group (CO2 fractional laser therapy) and an observation group (CO2 fractional laser therapy combined with subcutaneous separation followed by CGF filling), with 41 cases in each group. The efficacy assessed by the Goodman & Baron Scar Grading Scale, Visual Analogue Scale (VAS), treatment satisfaction, Global Aesthetic Improvement Scale (GAIS), Self-Rating Anxiety Scale (SAS), duration of erythema and edema, healing time, incidence of hyperpigmentation, time off work, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), and adverse reactions were recorded and compared between the two groups.Results: At 2 months post-treatment, the observation group exhibited lower Goodman & Baron scar grade scores, pre- and post-treatment difference scores, IL-6, TNF-α, and INF-γ levels compared to the control group (P0.05).Conclusion: Subcutaneous separation followed by CGF filling combined with CO2 fractional laser treatment demonstrates significant efficacy in treating atrophic scars, reducing long-term inflammatory responses and anxiety levels while enhancing patient satisfaction with treatment outcomes.