Objective: To understand the current status and equity of resource allocation in immunization clinics in Chengdu, and to provide a basis for health administrative departments to further optimize the allocation of immunization program resources. Methods: A questionnaire survey was conducted to assess the current status of human resource allocation. The Gini coefficient, agglomeration degree, and spatial autocorrelation analysis were employed to evaluate the equity of resource allocation. Results: In 2024, the number of vaccination personnel per 10,000 population in Chengdu was 1.07. The staff were predominantly female, with an age distribution of (36.60±7.62) years old. The proportions of personnel with official establishment, high academic qualifications, and senior professional titles were relatively low. Overall, the equity of immunization resource allocation based on population was superior to that based on geographical area. The resources in the first and second urban circles were well allocated or even surplus in terms of geographical distribution, while the third circle showed better allocation efficiency when measured by population. In terms of spatial distribution, the high-high clustering mainly radiated to surrounding areas with Qionglai City and Jianyang City in the third circle as the core. And the low-low clustering was primarily concentrated in the first urban circle and the northwestern part of the second circle. Conclusion: The resource allocation in vaccination clinics in Chengdu is irrational and unevenly distributed, necessitating further optimization.