Objective: To investigate the effects of labetalol combined with low-dose aspirin and vitamin D on miR-210, SIRT3 and placental blood perfusion in patients with gestational hypertension. Methods: 90 pregnant women with gestational hypertension were selected as the research subjects. Based on therapeutic regimens, they were allocated to either a control group (n=42) receiving labetalol plus low-dose aspirin, or a combination group (n =48) receiving additional vitamin D supplementation. miR-210, silencing information regulatory factor 3 (SIRT3), placental perfusion parameters \[pulsatility index(PI), resistance index (RI), and peak systolic/end diastolic flow velocity (S/D)\], as well as adverse pregnancy outcomes and adverse reactions were compared between the two groups. Results: The combination group exhibited lower miR-210 expression(P<0.05) and higher SIRT3 mRNA levels(P<0.05) in placental tissues relative to controls. Compared with the control group, the PI, RI, and S/D of placental blood perfusion parameters in combination group were lower(P<0.05). There was no statistically significant difference in the incidence of adverse pregnancy outcomes and adverse reactions between the two groups(P>0.05). Conclusion: Labetalol combined with low-dose aspirin and vitamin D can more effectively regulate the expression imbalance of miR-210 and SIRT3 in placental tissue of patients with hypertensive disorder complicating pregnancy, improve placental blood perfusion, and has good safety.