Ischemia/reperfusion injury is a syndrome characterized by structural and functional changes such as cell swelling and necrosis during rapid blood flow recovery after tissue ischemia, which result in aggravated tissue damage. Ischemia/reperfusion injury is associated with many organs such as heart, liver, brain, kidney. Cardiovascular disease is the most common cause of death in humans, and myocardial ischemia/reperfusion injury is strongly associated with cardiovascular disease. Currently, the research on myocardial ischemia/reperfusion injury has attracted much attention, but the exact mechanism is not fully understood. Hydrogen sulfide is an important gas molecule, it can regulate the equilibrium state of free radicals in the body, combat and alleviate damage caused by free radicals. As a result, hydrogen sulfide plays an important role in the pathophysiological processes of many diseases. In recent years, studies have found that H?S could protect myocardial ischemia/reperfusion injury caused by myocardial infarction, heart failure and other diseases through a variety of molecular mechanisms such as anti-oxidation, anti-apoptosis, anti-inflammation, anti-necrosis, etc., but the specific mechanism was not completely clear, especially the involved signaling pathways. In this paper, the protective mechanism of hydrogen sulfide against myocardial ischemia/reperfusion injury and the involved signaling pathways were reviewed, providing theoretical reference for further research.