Abstract:Objective:To clarify the differential expression of LINC01006 in Castration-Resistant Prostate Cancer.To prove that LINC01006 mediates the enhanced proliferation and invasiveness of prostate cancer cells.To clarify that LINC01006 is packaged into exosomes and delivered between cells,resulting in enhanced PCa cell proliferation and invasiveness.Methods:Screen the differentially expressed transcriptomes of normal prostate tissue and prostate cancer tissue by analyzing the biological information database.The ex-pression difference of LINC01006 in normal prostate tissue and cancer tissue was verified by RT-qPCR technology.Using the construc-tion of shRNA expression vector plasmid,combined with lentivirus coating technology,LNCaP and 22RV1 cells were transfected.Using the technology of extracting extracellular medium,by adding RNase and changing the permeability of the probe,it was verified that LINC01006 was covered by the membrane structure.PCa cell exosomes were extracted by high-speed ultracentrifugation and verified by Western Blot.The expression difference of LINC01006 in exosomes was verified by RT-qPCR technology.CCK-8 assay and colony for-mation assay were used to verify the changes of PCa cell proliferation and migration ability in the case of LINC01006 knockout.CCK-8 proliferation activity experiment and plate cloning experiment When exosomes derived from homologous and xenogeneic PCa cells were co-cultured,the recovery of PCa cell proliferation and migration ability in the case of LINC01006 knockout.Results:Compared with nor-mal prostate epithelial cells(RWPE-1),the expression of LINC01006 in LNCaP and 22RV1 cells was up-regulated(P＜0.01).The expression of LINC01006 in knockdown LNCaP was down-regulated by 84.7％compared with the control group(P＜0.01),and the expression of LINC01006 in knock-down 22RV1 was down-regated by 80.3％compared with the control group(P＜0.01).There was no significant difference between CM group and CM+RNase group(P＞0.05).Compared with the other two groups,the detection of LINC01006 in the CM+RNase+T group was significantly lower(P＜0.01).The exosomes were successfully extracted by high-speed uLtracentrifugation,and the specific proteins CD63,CD81 and HSP70 were highly expressed(P＜0.05).Exosomal LINC01006 expres-sion derived from LNCaP cells was significantly increased(P＜0.01).The expression of exosome LINC01006 derived from 22RV1 cells was significantly increased(P＜0.01).Compared to the control group(siCtrl),the absorbance of the knockout group(si1006)cells on the 3rd and 4th day was obviously lower than that of the control group(P＜0.01).Compared to the control group(siCtrl),the cells of the knockout group(siCtrl),the number of clones were significantly reduced(P＜0.05).As opposed to the control group(Ctrl),the absorbance of cells in the exosome group(LNCaP exosome)on the 4th day was obviously higher than that in the control group(P＜0.05).As opposed to the control group(Ctrl),the number of cell clones in the exosome group was significantly increased(P＜0.05).Compared with the control group(Ctrl),the absorbance of cells in the exosome group(22RV1 exosome)on the 3rd and 4th day was significantly bigger than which in the control group(P＜0.05).As opposed to the control group(Ctrl),the number of cell clones in the exosome group was significantly increased(P＜0.01).Conclusion:LINC01006 is highly expressed in PCa cell lines and can promote tumor proliferation and migration.LINC01006 is coated with exosomes and secreted into the extracellular space through PCa cells to exert corresponding biological activities.PCa-derived exosomes can induce LINC01006 PCa cells to restore their proliferative ca-pacity.

Abstract:Objective:To analyze the expression of CECR2 and the distribution of M2 macrophages in prostate cancer tissues,and to explore the relationship between the expression of CECR2 and the distribution of M2 macrophages in the progression of prostate cancer.Methods:Clinical and tissue specimen data of 98 inpatients who underwent prostate cancer surgery were collected.The expressions of cat-eye syndrome chromosome region candidate gene 2(CECR2)and CD163 antibody in prostate cancer tissues were detected by immunohis-tochemical staining,and the relationship between their expressions and clinicopathological features was analyzed.Results:CECR2 was highly expressed in benign prostate tissues,with a positive rate of 87.76％,while it was lowly expressed in prostate cancer tissues,with a positive rate of 28.57％.The positive rate of CECR2 in prostate cancer tissues was lower than that in adjacent benign prostate tissues(P＜0.05).The low expression of CECR2 in prostate cancer tissues was closely related to the presence of nerve invasion,extraprostatic spread,intraductal carcinoma,tumor grade and T stage(P＜0.05).The median number of CD163-positive macrophages(M2 macropha-ges)in prostate cancer tissues was 21.03 per high-power field,while that in adjacent benign prostate tissues was 4.23 per high-power field.The infiltration density of CD163-positive macrophages in cancer tissues was higher than that in adjacent benign prostate tissues.Further analysis of the relationship between the expression of CECR2 and the distribution of M2 macrophages showed that 47 of the 70 ca-ses with low expression of CECR2 had high-density distribution of M2 macrophages(CD163 positive),and the low expression of CECR2 was positively correlated with high-density distribution of M2 macrophages.Conclusion:There is a significant downregulation of CECR2 expression in prostate cancer tissues,and its low expression is related to the infiltration density of M2 macrophages.CECR2 may induce M2 macrophage polarization,regulate the tumor immune microenvironment,and thereby promote the progression of prostate cancer.

Abstract:Objective:To investigate the protective effect of celecoxib on retinopathy(DR)in diabetic rats based on hypoxia-in-ducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods:30 male Wistar rats were ran-domly divided into normal control group,model group and celecoxib group,with 10 rats in each group.In addition to the normal control group,the other two groups were fed with high-fat diet+streptozotocin intraperitoneal injection to construct DR model.After successful modeling,the celecoxib group was given intragastric administration of celecoxib,while the model group and the normal control group re-ceived equal volumes of solvent once a day for 4 weeks.On the day of drug intervention and 4 weeks after intervention,the body weight and fasting blood glucose of rats were measured.The levels of serum VEGF,interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were measured by enzyme-linked immunosorbent assay.The pathological changes of rat retina were observed by hematoxylin-eosin(HE)staining.The expression of VEGF and HIF-1α protein in rat retina was detected by Western blot.Results:On the day of drug in-tervention and after 4 weeks of intervention,compared with the normal control group,the body weight of the model group and the cele-coxib group decreased significantly(P＜0.05),and the FPG increased significantly(P＜0.05).Compared with the normal control group,the levels of serum VEGF,IL-6 and TNF-α in the model group were significantly increased(P＜0.05).Compared with the mod-el group,the serum levels of VEGF,IL-6 and TNF-α in the celecoxib group were significantly decreased(P＜0.05).HE staining showed that the retinal tissue cells in the normal control group were arranged neatly,the retinal nerve cells in the model group were dis-ordered,and the inner nuclear layer and the outer nuclear layer showed obvious loose changes.Compared with the model group,the ar-rangement of retinal nerve cells in the celecoxib group was closer to normal,and the loose degeneration of the inner nuclear layer and the outer nuclear layer was reduced.Compared with the normal control group,the relative expression of VEGF and HIF-1α protein in the retinal tissue of the model group was significantly increased(P＜0.05).Compared with the model group,the relative expression of VEGF and HIF-1α protein in the retinal tissue of the celecoxib group decreased significantly(P＜0.05).Conclusion:Celecoxib has a protective effect on retinopathy in diabetic rats,and its mechanism may be related to the inhibition of HIF-1α/VEGF signaling pathway activation.

Abstract:Objective:To compare the efficacy of intravitreal injection of ozurdex and aflibercept in the treatment of cystoid mac-ular edema(CME)caused by diabetic retinopathy(DR)and its influence on visual field gray value,macular thickness and inflamma-tory factors.Methods:The case data of 92 patients(164 eyes)with CME caused by DR were collected,and the above patients were di-vided into ozurdex group(n=47,79 eyes)and aflibercept group(n=45,85 eyes)by treatment methods.The ozurdex group was given injection of ozurdex(0.7 mg),while the aflibercept group was given injection of aflibercept(2 mg).The efficacy,visual acuity,visual field,macular parameters,inflammation and biochemical indexes were evaluated after 3 months of treatment,and the incidence rates of adverse events in the two groups during treatment were counted.Results:The efficacy in ozurdex group was better(P＜0.05),and the light sensitivity was higher than that in aflibercept group(P＜0.05),and the BCVA LogMAR value,visual field gray value,central macular thickness(CRT),total macular volume(TMV),interleukin-1 β(IL-1 β),interleukin-23(IL-23)and monocyte chemoattrac-tant protein-1(MCP-1)were lower(P＜0.05).However,the levels of vascular endothelial growth factor(VEGF)and stromal cell-de-rived factor 1(SDF-1)in aflibercept group were lower(P＜0.05).There were no significant differences in the total incidence rates of adverse events(P＞0.05).Conclusion:Compared with aflibercept,ozurdex has better efficacy in the treatment of CME caused by DR.Ozurdex has rapid visual acuity recovery,obvious improvement of visual field and relieved inflammatory response,but the effect of inhib-iting neovascularization is slightly worse than that of aflibercept.

Abstract:Objective:To analyze the relationship between serum lipoprotein-associated phospholipase A2(Lp-PLA2)and ho-mocysteine(Hcy)levels and condition,outcomes in elderly patients with hypertension and acute cerebral infarction(ACI).Methods:A total of 107 elderly patients with hypertension and ACI were selected as the observation group.Meanwhile,100 healthy individuals were selected as the control group.Patients in the observation group were grouped according to the severity and outcome of the disease.The relationship between serum Lp-PLA2,Hcy levels and the severity and outcome of the disease was analyzed.Results:Compared with control group,the levels of Lp-PLA2 and Hcy were higher in the observation group(P＜0.05).The levels of Lp-PLA2 and Hcy in the severe group,the moderate group,and the mild group decreased in order(P＜0.05).The levels of Lp-PLA2 and Hcy in patients with poor outcomes were higher than those in patients with good outcomes(P＜0.05).Serum Lp-PLA2 and Hcy levels were positively corre-lated with the severity and outcome of the disease(P＜0.05).The AUCs of serum Lp-PLA2,Hcy,and their combination for evaluating moderate to severe condition were 0.786,0.861,and 0.916.The AUCs for predicting disease outcomes were 0.767,0.667,and 0.801.Conclusion:Serum Lp-PLA2 and Hcy levels in elderly patients with hypertension and ACI are elevated.The two can well help to eval-uate the patient's condition and predict disease outcome.

Abstract:Objective:To analyze the application value of breast ultrasound artificial intelligence S-Detect technology in the diag-nosis of BI-RADS 4 breast masses.Methods:Totally 102 patients diagnosed with BI-RADS 4 breast masses(102 masses)by conven-tional ultrasound were reviewed.All of them underwent detection using breast ultrasound S-Detect technology.Histopathological results were used as the gold standard to compare the diagnostic efficacy of conventional ultrasound and breast ultrasound S-Detect technology for BI-RADS 4 breast masses.Results:Among the 102 cases enrolled,62 cases were confirmed by pathology,including 33 cases with benign masses and 29 cases with malignant masses.The sensitivity,specificity,accuracy,positive and negative predictive values of breast ultrasound S-Detect technology for diagnosing benign and malignant BI-RADS 4 breast masses(93.10％,96.97％,95.16％,96.43％and 96.12％)were higher than those of conventional ultrasound(72.41％,78.79％,75.81％,75.00％and 76.47％)(P＜0.05).The AUC of breast ultrasound S-Detect technology was 0.874,which was larger than that of conventional ultrasound(0.718)(Z=2.274,P=0.023).Conclusion:Breast ultrasound S-Detect technology has high value in differential diagnosis of benign and malignant BI-RADS 4 breast masse and is worthy of clinical promotion.

Abstract:Objective:To explore the therapeutic effect of transnasal endoscopic sinus opening on patients with chronic sinusitis and nasal polyps and analyze the related factors affecting the recovery of operative cavity mucosa membrane.Methods:Retrospective a-nalysis was performed on the data of 208 patients with chronic sinusitis and nasal polyps who received endoscopic sinus opening.The operative effect and operative cavity mucosa membrane recovery were analyzed.According to the recovery status of operative cavity mu-cosa membrane,the patients were divided into recovery group and non-recovery group,and the independent risk factors affecting the re-covery of operative cavity mucosa membrane were analyzed.Results:Among 208 patients with chronic rhinosinusitis and nasal polyps,152 cases(73.08％)were cured,34 cases(16.35％)were improved,22 cases(10.58％)were ineffective.6 months after operation,152 cases of nasal mucosa were completely restored to normal,56 cases were not completely restored to normal.The proportions of clini-cal type Ⅱ-Ⅲ,nasal mucosa injury after trimming nose hair and asthma,VAS score,Lund-Mackay score,Lund-Kennedy score,peripher-al blood EOS and tissue EOS in incomplete normal recovery group were higher than those in complete normal recovery group(P＜0.05)while the proportions of postoperative regular nasal irrigation and postoperative regular use of hormones were lower than those in complete normal recovery group(P＜0.05).Logistic regression analysis showed that clinical typing,VAS score,Lund-Mackay score,Lund-Kennedy score,tissue EOS,postoperative regular nasal irrigation and postoperative regular use of hormones were independ-ent related factors affecting the recovery of operative cavity mucosa membrane(P＜0.05).Conclusion:Transnasal endoscopic sinus o-pening is effective in the treatment of chronic sinusitis with nasal polyps.The recovery of operative cavity mucosa membrane is affected by a variety of clinical and pathological factors.And postoperative regular nasal irrigation and use of hormones help the nasal mucosa to return to normal.

Abstract:Objective:To investigate the effect of combined treatment of minocycline hydrochloride and cefuroxime axetil tablets on periodontal function in patients with periodontal lesions.Methods:120 patients with pulp and periodontal lesions were divided into a control group(n=60,basic periodontal treatment+cefuroxime axetil treatment)and an observation group(n=60,control group+mi-nocycline hydrochloride)according to different intervention methods,both treated for4 weeks.The therapeutic efficacy,periodontal clin-ical indices,pain(VAS)score,gingival crevicular fluid inflammatory factors(interleukin-1 β,prostaglandin E2(PGE2),and interleu-kin-8(IL-8)],and the incidence of adverse reactions were compared between the two groups.Results:The overall response rate in the observation group was higher than that in the control group(P＜0.05).After 4 weeks of treatment,both groups exhibited a reduction in gingival bleeding index,probing depth,plaque index,VAS scores,IL-1 β,PGE2,and IL-8(P＜0.05),and the observation group was lower than the control group(P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P＞0.05).Conclusion:The combined treatment of minocycline hydrochloride and cefuroxime axetil tablets in patients with periodontal lesions is effective,can effectively improve periodontal function,relieve pain,and reduce inflammatory reactions,and is worth promoting.

Abstract:Objective:To explore the influence of Shenfukang on renal function and levels of transforming growth factor-β1(TGF-β1)and connective tissue growth factor(CTGF)in patients with stage 2～4 chronic kidney disease(CKD)of spleen-kidney deficiency and stasis-toxin internal accumulation.Methods:A total of 98 patients with stage 2～4 CKD were selected,and were divided into control group(n=49)and observation group(n=49)by means of different treatment regimens.The control group was treated with conventional western medicine,whereas the observation group was added with Shenfukang granules on this basis.The observation period was 12 weeks.The renal function indexes[blood urea nitrogen(BUN),serum albumin(ALB),serum creatinine(Scr)and uric acid(UA)],blood lipids[total cholesterol(TC)and triglyceride(TG)],hemorheology indexes[whole blood high-shear viscosity(WHV),whole blood low-shear viscosity(WLV),plasma viscosity(PV)and erythrocyte aggregation index(EAI)]and serum factors(TGF-β1 and CTGF)before and after medication and clinical efficacy were compared between the two groups.Results:Compared with before medication,the levels of BUN,Scr and UA,WHV,WLV,PV and EAI,TGF-β1 and CTGF after 12 weeks of medication were re-duced in both groups(P＜0.05),and the above indexes in the observation group were lower compared with those in the control group(P＜0.05),and there were no significant differences in ALB,TC and TG levels between groups(P＞0.05).After 12 weeks of medica-tion,the total effective rate of treatment was higher in the observation group compared to the control group(91.84％vs.77.55％,P＜0.05).Conclusion:Shenfukang can protect renal function,improve hemorheology and reduce the levels of TGF-β1 and CTGF in patients with stage 2-4 CKD of spleen-kidney deficiency and stasis-toxin internal accumulation.

Abstract:Objective:To explore the correlation between serum homocysteine(Hcy),score of carotid plaque vulnerability and white matter lesion,cognitive dysfunction in elderly patients with cerebral small vascular disease(CSVD).Methods:A total of 126 elderly patients with CSVD were enrolled as the research objects.According to cognitive function level,they were divided into impair-ment group(n=66)and non-impairment group(n=60).The general clinical data,level of serum Hcy,scores of carotid plaque vul-nerability,white matter lesion grading(Fazekas)and cognitive function[Mini-Mental State Examination(MMSE)]in the two groups were compared.According to the Fazekas score of all patients,they were divided into a group with white matter lesions and a group with normal white matter.The serum Hcy levels and carotid plaque vulnerability scores of the two groups were compared,the correlation be-tween level of serum Hcy,score of carotid plaque vulnerability and Fazekas score and MMSE score was analyzed.Results:Compared with non-impairment group,age,proportions of hypertension and dementia family history,levels of serum D-dimer and Hcy,scores of ca-rotid plaque vulnerability and Fazekas were higher in impairment group(P＜0.05),MMSE score was lower(P＜0.05).Compared with the normal white matter group,the level of serum Hcy and score of carotid plaque vulnerability were higher in the white matter le-sion group(P＜0.05).The level of serum Hcy and score of carotid plaque vulnerability were positively correlated with Fazekas score,while negatively correlated with MMSE score(P＜0.05).Conclusion:The level of serum Hey and score of carotid plaque vulnerabili-ty are positively correlated with white matter lesion and cognitive dysfunction in elderly patients with CSVD.

Abstract:Objective:To observe the clinical efficacy of enhanced external counterpulsation(EECP)on community patients with coronary heart disease.Methods:80 patients who were referred back to the community for rehabilitation treatment after treatment in the superior hospital were selected to participate in the study,and were divided into control group and treatment group according to dif-ferent rehabilitation methods,with 40 cases in each group.The control group was given drug combined with routine rehabilitation exer-cise therapy,while the treatment group was treated with EECP rehabilitation therapy on this basis.The differences in cardiac reserve in-dexes,pulmonary reserve indexes and exercise tolerance indexes before and after treatment and differences in quality of life(MLHFQ score)and sleep quality(PSQI score)before treatment and after 1,2 and 3 months of treatment were compared between the two groups.Results:After treatment,the levels of cardiopulmonary reserve indexes and exercise tolerance indexes in both groups were signif-icantly improved compared with those before treatment(P＜0.05),and the left ventricular ejection fraction(LVEF),stroke volume(SV),pulmonary reserve indexes and exercise tolerance indexes in treatment group improved more significantly compared to control group(P＜0.05).After 1,2,and 3 months of treatment,the MLHFQ score and PSQI score were decreased in both groups of patients(P＜0.05),and the scores in treatment group at each time point were lower than those in control group(P＜0.05).Conclusion:EE-CP can effectively improve the cardiopulmonary function and exercise tolerance,enhance the quality of life and sleep quality in commu-nity patients with coronary heart disease,and is worthy of promotion and application.

Abstract:Objective:To investigate the effect of dexamethasone combined with ropivacaine for brachial plexus block on analge-sia in patients with distal radius fractures,and the effect on inflammatory factors.Methods:A total of 110 patients with distal radius fracture were divided into control group and observation group according to the different ways of intervention,each group was 55 cases.Both groups underwent brachial plexus block.The control group was 0.25％ropivacaine,and the observation group was dexamethasone 10 mg+0.25％ropivacaine.The liquid volume of the two groups was 20 mL.The duration of sensory block,postoperative pain score(VAS score),postoperative remedial analgesia rate,postoperative hospitalization time and adverse reactions were recorded in the two groups.The serum interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels were detected.Results:Compared with the control group,the duration of sensory block in the observation group was longer(P＜0.05).The pain scores of the observation group at rest and activity at each time point from 2 to 48 h after operation were decreased(P＜0.05),and the remedial analgesia rate at 48 h after operation was lower(P＜0.05).At 24 h after operation,the serum IL-6 and TNF-α levels in the observation group was lower comparing with the control group(P＜0.05).The postoperative hospital stay in the observation group was shorter comparing with the control group(P＜0.05).There was no statistically significant difference in the occurrence of adverse reactions between the two groups of patients(P＞0.05).Conclusion:Dexamethasone combined with ropivacaine for brachial plexus block can improve the postoperative analgesic effect of patients with distal radius fractures,reduce inflammatory stress,and facilitate postoperative recovery.

Abstract:Objective:To analyze the influencing factors of ultrasound-guided anhydrous ethanol interventional therapy in the treatment of ovarian endometriosis cyst(OEC).Methods:The clinical data of 109 patients with OEC were retrospectively analyzed.Ac-cording to the cyst volume reduction rate and symptom relief status at 6 months after treatment,the patients were divided into effective group(cyst volume reduction ≥ 50％and significant symptom improvement,n=82)and ineffective/partially effective group(cyst vol-ume reduction＜50％or not significant symptom improvement,n=27).Univariate and multivariate Logistic regression analyses were used to screen the influencing factors of the efficacy.Results:Univariate analysis showed that age,cyst diameter,cyst wall thickness,number of cysts,loculated cyst,side of cyst,history of endometriosis surgery,uterine fibroids or infertility,postoperative gonadotropin-re-leasing hormone agonist(GnRH-a)use,postoperative residual mass and preoperative serum CA125 level were related to efficacy(P＜0.05).Multivariate Logistic regression analysis suggested that age,cyst diameter,cyst wall thickness,loculated cyst,cyst side,postoper-ative residual mass and preoperative serum CA1 25 were independent risk factors,while postoperative GnRH-a use was a protective fac-tor(P＜0.05).Conclusion:Older age,thick cyst wall thickness,loculated cyst,postoperative residual mass and elevated preoperative serum CA125 level are risk factors affecting the efficacy of ultrasound-guided anhydrous ethanol interventional therapy for OEC.

Abstract:Objective:To explore the clinical effects of intravenous chemotherapy combined with IPHC on patients with advanced ovarian cancer(AOC).Methods:Totally 100 patients with AOC were divided into two groups by means of different intervention meth-ods.50 patients in the control group were treated with paclitaxel+carboplatin for intravenous chemotherapy,and the other 50 patients in the observation group were combined with IPHC on the basis of the control group.The clinical efficacy,tumor markers[carcinoembryon-ic antigen(CEA),alpha-fetoprotein(AFP),basic fibroblast growth factor(bFGF),human epididymis protein 4(HE4)],immune cell indicators(CD3+T cell,CD4+T cell,CD4+/CD8+T cell ratio),adverse reactions and survival status were compared between the two groups.Results:After treatment,compared with the control group,the total clinical control rate in the observation group was higher(P＜0.05).The levels of CEA,AFP,bFGF and HE4 in the observation group were lower compared with those in the control group(P＜0.05).The levels of CD3+T cell,CD4+T cell and CD4+/CD8+T cell ratio were higher in the observation group(P＜0.05).There was no statistical significance in the incidence of adverse reactions between groups(P＞0.05).After 12 months of follow-up,the survival curve revealed no statistical difference between both groups(P＞0.05).Conclusion:IPHC combined with TC intravenous chemotherapy has certain efficacy on patients with AOC,and it can reduce the tumor markers and enhance the immune function,and it will not additionally increase adverse reactions.

Abstract:Objective:To explore the effectiveness and safety of bupivacaine liposome for fascia iliaca block on postoperative an-algesia in elderly patients undergoing hip surgery.Methods:90 patients were included in the research subjects,and were divided into group A(n=30),group B(n=30),and group C(n=30)according to intervention methods.Fascia iliaca block was performed in all three groups,with 0.5％lidocaine given in group A,0.25％bupivacaine hydrochloride given in group B,and bupivacaine liposome(266 mg)given in group C.The systolic blood pressure(SBP),diastolic blood pressure(DBP),heart rate(HR),pain score(NRS)and pain factors[substance P(SP),prostaglandin E2(PGE2),serotonin(5-HT)]were compared at different time points,and the ad-verse reactions were recorded.Results:The analgesic pump compression frequency and dosage of opioids were lower in groups B and C than those in group A(P＜0.05).The SBP,DBP and HR in the three groups 10 min after anesthesia were reduced compared with those before anesthesia(T0),and the group C had higher DBP than group A(P＜0.05).At 15 min(T2)and 30 min(T3)after an-esthesia,the group C had higher SBP than group A(P＜0.05),and higher HR than groups A and B(P＜0.05).The NRS scores in groups B and C were lower than that in group A at different time points after surgery(P＜0.05).The levels of SP,PGE2 and 5-HT in the three groups after surgery were risen compared with those before surgery(P＜0.05).The SP and 5-HT levels in groups B and C were lower than that in group A(P＜0.05),and the PGE2 level was lower in group C than that in groups A and B(P＜0.05).The in-cidence of nausea and vomiting was lower in group C than that in groups A and B(P＜0.05).Conclusion:Fascia iliaca block with bupivacaine liposome can improve the analgesia effect and reduce the levels of pain factors in elderly patients undergoing hip surgery,and it has good safety and has certain clinical application value.

Abstract:Objective:Based on Academician Tong Xiaolin's academic thought of"differentiation and treatment of state and tar-get",to observe the effect of Xuanfei Pingchuan Decoction in treating COPD with phlegm-turbidity obstructing lung syndrome.Meth-ods:60 patients with COPD were selected and divided into two groups according to different treatment methods,with 30 cases in each group.The control group was treated with conventional Western medicine,and other group was given Xuanfei Pingchuan decoction on this basis.The TCM syndrome score,lung function,blood gas analysis and the mMRC were compared between the two groups before and after treatment.Results:After treatment,the total effective rate of the observation group was higher than that of the control group(P＜0.05).After treatment,traditional Chinese medicine syndrome score,mMRC score,FEV1 and FEV1/FVC indexes of lung function,PaO2 and PaCO2 indexes of blood gas analysis in the observation group were better than those in the control group(P＜0.05).Conclu-sion:According to the"differentiation and treatment of state target",Xuanfei Pingchuan Decoction has a remarkable effect on COPD by modulated targeting.It can obviously reduce the clinical symptoms of patients,increase the activity endurance,improve the quality of life,and delay the progression of the disease.

Abstract:Objective:To analyze the effect of different frequencies of extracorporeal shock wave lithotripsy(EWSL)in the treatment of ureteral calculi.Methods:564 patients with ureteral stones were included and divided into 282 cases each in the intermedi-ate frequency group and the high frequency group according to the different modes of intervention,and were treated with shock waves at frequencies of 35 times/min and 60 times/min,respectively.The stone fragmentation time,3-month postoperative stone removal rate,and stone clearance rate,preoperative and postoperative 24-hour pain level(VAS score),renal blood flow indicators[renal pulsatility index(PI),renal artery resistance index(RI),and maximum renal artery flow velocity(Vmax)],renal function indicators[urea nitrogen(BUN),urinary creatinine(Cr),and glomerular filtration rate(GFR)levels],and incidence of complications within 3 months after surgery were compared between the two groups.Results:The stone removal rate and stone clearance rate in intermediate frequency group were higher than those in high frequency group at 3 months after surgery(P＜0.05),and the lithotripsy time was shorter in high frequency group than that in intermediate frequency group(P＜0.05).At 6 h,1 d,3 d,and 2 w after surgery,the VAS score in the two groups was decreased after surgery(P＜0.05),and the score in intermediate frequency group was lower than that in high frequency group(P＜0.05).24 h affter surgery,both groups of patients showed a decrease in PI and Vmax(P＜0.05),and high frequency group was lower than the intermediate frequency group(P＜0.05).RI increased in both groups(P＜0.05),and the high frequency group was higher than the intermediate frequency group(P＜0.05).The levels of BUN and Cr both increased(P＜0.05),and the high frequency group was higher than the intermediate frequency group(P＜0.05).The GFR levels were all reduced(P＜0.05),and the high frequency group was lower than the intermediate frequency group(P＜0.05).The complications were lower in intermediate frequency group than those in high frequency group(P＜0.05).Conclusion:Shock wave therapy at 35 times/min and 60 times/min have good effects in the treatment of ureteral calculi,but 35 times/min frequency shock wave is better in enhancing the stone clearance rate and relieving the pain degree,and it has smaller influence on renal function and it is safer and more effective.

Abstract:Objective:To explore the effects of pedicle screw placement through posterior median approach,Wiltse intermuscular space approach and percutaneous approach on perioperative indexes and prognosis in patients with single-segment thoracolumbar frac-ture.Methods:According to different surgical methods,90 patients with single-segment thoracolumbar fracture in the hospital were di-vided into posterior median approach group(n=34),Wiltse approach group(n=28)and percutaneous approach group(n=28).The perioperative indexes,bone metabolism indexes,serum muscle enzymes,thoracolumbar recovery and postoperative complications were compared among the three groups.Results:In Wiltse approach group,percutaneous approach group and posterior median approach group,operation time,length of hospital stay and incision length were gradually increased(P＜0.05).At 48 h after surgery,levels of osteocalcin(OC),osteoprotegerin(OPG)and procollagen type Ⅰ N-terminal propeptid(PINP)were gradually decreased in Wiltse ap-proach group,percutaneous approach group and posterior median approach group(P＜0.05).The levels of serum creatine kinase in Wiltse approach group and percutaneous approach group was lower than that in posterior median approach group(P＜0.05).At 3 months and 1 year after surgery,Wiltse approach group had the best recovery effect on thoracolumbar spine(P＜0.05).There was no significant difference in the total incidence of postoperative complications among the three groups(P＞0.05).Conclusion:Among the three methods,curative effect of Wiltse intermuscular space approach is better in single-segment thoracolumbar fracture,with low inci-dence of complications.

Abstract:Objective:To analyze the colonization status of Haemophilus influenzae in nasal cavity and oropharynx of children with Mycoplasma pneumoniae pneumonia(MPP)and its clinical significance.Methods:In 80 children with MPP,multiple polymerase chain reaction(PCR)and NGS sequencing were used to detect the common bacteria in the nasal cavity and oropharynx of the enrolled children,and the colonization status of Haemophilus influenzae in children with MPP of different ages and genders was analyzed.Ac-cording to the results of oropharyngeal bacteria detection,the children were divided into MPP group(negative oropharyngeal bacteria)and MPP+bacteria group(positive oropharyngeal bacteria).The clinical characteristics and treatment methods of the two groups were compared.Results:A total of 452 strains of bacteria were isolated from nasal and oropharyngeal secretions of 80 children with MPP.Haemophilus influenzae was the most common colonized strain,and 163 strains were isolated,accounting for 36.06％.Other major strains included Streptococcus pneumoniae(98 strains,21.68％),Staphylococcus aureus(83 strains,18.36％),Escherichia coli(46 strains,10.18％)and Klebsiella pneumoniae(25 strains,5.53％).The colonization rate of Haemophilus influenzae in children aged 1～3 years was the highest,reaching 76.67％(P＜0.05).The colonization rate of Haemophilus influenzae in boys(64.44％)was higher than that in girls(40.00％)(P＜0.05).The duration of fever,duration of cough and hospitalization time in MPP+bacteria group were significantly longer than those in MPP group(P＜0.05).Compared with the MPP+bacterial group,the duration of antibiot-ic use in the MPP group was significantly shorter(P＜0.05),and the proportion of atomization treatment was lower(P=0.027＜0.05).There were no statistical differences in the use of fiberoptic bronchoscopy and use of oxygen therapy between both groups(P＞0.05).Conclusion:There is a high colonization rate of Haemophilus influenzae in nasal cavity and oropharynx of children with MPP,and the colonization status of children with MPP is closely related to the aggravation of the disease,the prolongation of hospitalization time and the increase of treatment demand.

Abstract:Objective:To explore the clinical effect of endoscopic mucosal resection(EMR)and endoscopic submucosal dissec-tion(ESD)in the treatment of early colorectal cancer and precancerous lesions and its influence on postoperative complications.Meth-ods:Clinical data of 92 patients with early colorectal cancer and precancerous lesions were retrospectively analyzed.According to differ-ent treatment methods,the patients were divided into EMR group and ESD group.Propensity score matching method was applied to elim-inate the confusion bias caused by baseline data differences.Finally,41 pairs of patients with comparable baseline data were obtained from each group.The surgical time,postoperative hospital stay,lesion resection rate(en bloc resection rate,complete resection rate)and postoperative complications,inflammatory response indicators[C-reactive protein(CRP),white blood cell(WBC),interleukin-6(IL-6)]before surgery and at 24 hours after surgery,levels of tumor markers[carcinoembryonic antigen(CEA),carbohydrate antigen 199(CA199),carbohydrate antigen 242(CA242)]and fecal occult blood test before surgery and at 3 months after surgery and postop-erative recurrence,metastasis and survival after 1 year of follow-up were compared between both groups.Results:The surgical time and en bloc resection rate in ESD group were longer or higher than those in EMR group(P＜0.05).The incidence rates of complications were higher in ESD group than those in EMR group(P＜0.05).At 24 hours after surgery,the levels of CRP,WBC and IL-6 in both groups were increased(P＜0.05),but the levels in ESD group at 24 hours after surgery were lower than those in EMR group(P＜0.05).The tumor markers levels and fecal occult blood test were declined in both groups at 3 months after surgery(P＜0.05).After 1 year of follow-up,the total incidence rate of postoperative local recurrence or distant metastasis was 9.76％in ESD group and 2.44％in EMR group(P＞0.05).There was no difference in progression-free survival between the two groups at 12 months after surgery(log rank x2=1.924,P=0.165).Conclusion:The overall effect of ESD and EMR is similar in the treatment of early colorectal cancer and precancerous lesions,and ESD has higher lesion en bloc resection rate and lower recurrence risk,and there is a certain risk of complica-tions and it is necessary to select the appropriate method according to the patient's situation.

Abstract:Objective:To compare the effects of transabdominal laparoscopic nephron sparing surgery(LNSS)and retroperitone-al LNSS on perioperative indicators,renal function indicators,and inflammatory factors.Methods:96 patients with renal tumors who un-derwent LNSS were selected.According to different surgical approaches,the patients were assigned to the transabdominal group(n=46,transabdominal LNSS)and the retroperitoneal group(n=50,retroperitoneal LNSS).The two groups were compared regarding perio-perative indicators,renal function levels,inflammatory factors,and tumor markers.Results:Surgery time,warm ischemia time,intraoper-ative blood loss,and hospital stay of the retroperitoneal group were shorter and less than those of the transabdominal group(P＜0.05).The levels of blood urea nitrogen(BUN)and serum creatinine(Scr)in the retroperitoneal group were lower than those in the transab-dominal group at 1 month and 3 months after surgery(P＜0.05).Serum C-reactive protein(CRP),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels in the retroperitoneal group were lower than those in the transabdominal group(P＜0.05).Serum carbohydrate antigen 50(CA50)and carcinoembryonic antigen(CEA)levels in both groups on day 3 after surgery were lower than preoperative levels(P＜0.05),but there was no significant difference between groups(P＞0.05).There was no significant difference in the total incidence of postoperative complications between groups(P＞0.05).Conclusion:Compared with transabdominal LNSS,ret-roperitoneal LNSS has advantages in shorter surgery time,warm ischemia time,hospital stay,intraoperative blood loss.It also can help to improve renal function and inflammatory factor levels in patients with renal tumors,with a similar incidence of complications of postoper-ative complications.

Abstract:Objective:To compare the effects of manual release assisted arthroscopic surgery in patients with rotator cuff tears and frozen shoulder at different injury times.Methods:A retrospective analysis was conducted on 86 patients with rotator cuff tears and frozen shoulder,patients were divided into two groups based on the time since injury:group A(rotator cuff tear＜3 months,n=44)and group B(3 months ≤ rotator cuff tear ≤6 months,n=42).Both groups underwent MUA-assisted arthroscopic surgery.Surgical in-dicators(operation time and intraoperative blood loss),pain levels before and after treatment,range of motion of the shoulder joint(in-cluding forward flexion and external rotation),University of California Los Angeles(UCLA)Shoulder Score,American Shoulder and El-bow Surgeons(ASES)score,and complications were compared between the two groups.Results:There were no significant differences in surgical indicators between group A and group B(P＞0.05).At 1 and 3 month postoperatively,both groups showed reduced Visual Analog Scale(VAS)pain scores(P＜0.05),increased range of shoulder motion(P＜0.05),and improved UCLA and ASES scores(P＜0.05).Furthermore,at 1 month postoperatively,group A had lower VAS pain scores(P＜0.05),greater range of shoulder motion(P＜0.05),and higher UCLA and ASES scores(P＜0.05)compared to group B.At 3 months postoperatively,there were no signifi-cant differences in VAS pain scores,range of shoulder motion,and UCLA and ASES scores between group A and group B(P＞0.05).No nerve injuries,infections,or complications related to MUA were observed in either group postoperatively.Conclusion:Compared to patients with rotator cuff tears and frozen shoulder who have the injury for 3 to 6 months,those with an injury duration of less than 3 months showed more significant early symptom relief and better shoulder mobility when treated with MUA-assisted arthroscopic surgery,although long-term outcomes tend to be consistent.

Abstract:Objective:To analyze the effects of laparoscopic cholecystectomy(LC)combined with laparoscopic transcystic bile duct exploration and stone removal(LTCBDE)on clinical efficacy and immune function in elderly patients with gallbladder stones(GS)complicated by secondary common bile duct stones(CBDS).Methods:72 elderly patients with GS complicated by CBDS were divided into experimental and control groups according to different treatment methods,with 36 patients in each.The control group under-went LC+common bile duct incision stone removal with T-tube drainage(LCHTD),while the experimental group underwent LC+LTCBDE.The clinical efficacy and the impact of these two surgical methods on patients'immune function and inflammatory indicators were compared between the two groups.Results:There was no statistically significant difference in the total effective rate of treatment between the two groups(P＞0.05).The experimental group showed lower operative time,blood loss,hospital stay,and medical expen-ses compared to the control group(P＜0.05).The immune function indicators(CD3+,CD4+,CD8+T cell percentages and CD4+/CD8+ratio)in both groups were lower 3 days after surgery compared to pre-surgery(P＜0.05),but the experimental group had higher immune function indicators compared to the control group(P＜0.05).The inflammatory indicators(TNF-α,IL-10,CRP levels)in both groups were higher 3 days after surgery compared to pre-surgery(P＜0.05),but the experimental group showed lower inflammato-ry indicators compared to the control group(P＜0.05).Conclusion:LC+LTCBDE is an effective treatment for elderly patients with GS complicated by CBDS,which can effectively shorten hospital stay,reduce medical expenses,mitigate surgical impact on patient im-mune function,and result in a lighter inflammatory response.

Abstract:Objective:To analyze the differences of cerebral cortical neural activity in patients with chronic subjective tinnitus of different genders,explore the relationship between the objective index of cerebral cortical neural activity in patients with chronic subjec-tive tinnitus and the score of subjective THI scale,and establish the clinical value of HbO concentration in evaluating the severity of chronic subjective tinnitus patients.Methods:300 patients with chronic subjective tinnitus were selected as the experimental group,and 300 patients without tinnitus who were matched with the experimental group during the same period as the control group.All subjects were tested for the average concentration of HbO in the cerebral cortex using functional near-infrared spectroscopy(fNIRS).The differ-ences in HbO levels between subjects of different genders and their correlation with THI scale scores were analyzed.Results:In the ex-perimental group,the mean concentration of HbO in the frontal polar cortex(FPC-HbO)was higher than that in the control group,and the mean concentration of HbO in the dorsolateral prefrontal cortex(DPFC-HbO)was lower than that in the control group(P＜0.05).The FPC-HbO of female experimental group was higher than that of male group,and the DPFC-HbO was lower than that of male group(P＜0.05).There was a correlation between HbO and THI in the experimental group of female chronic subjective tinnitus patients with tinnitus duration less than 12 months and tinnitus frequency greater than 4 kHz(P＜0.05).Conclusion:Compared with the non-tinni-tus population,patients with chronic subjective tinnitus have increased FPC neural activity and decreased DPFC neural activity.Neural activity of FPC may be an objective measure of the degree of tinnitus impact in women with chronic subjective tinnitus with a tinnitus duration of less than 12 months and a tinnitus frequency greater than 4 kHz.

Abstract:Objective:To evaluate the therapeutic effect of dual needle bipolar pulsed radiofrequency therapy under ultrasound combined with CT dual guidance for elderly patients with herpes zoster neuralgia.Methods:60 elderly patients with thoracic and lumbar herpes zoster neuralgia were selected and divided into observation group and control group according to different treatment methods,with 30 cases in each group.The observation group was treated with double-needle bipolar pulsed radiofrequency operation,the control group was treated with single-needle unipolar pulsed radiofrequency operation.Pain was evaluated by pain VAS score before operation,the 3 day after operation,the 1 month after operation and the 3 month after operation.GQOL-74 was used to evaluate the quality of life before operation and 3 month after operation.The dosage of gabapentin before operation,the 3 day after operation,the 1 month after operation and the 3 month after operation were counted,and the operation time,the incidence of PHN and the incidence of adverse reactions were also counted.Results:At different time points after operation,the VAS scores and the dosage of gabapentin of the two groups were lower than those before operation(P＜0.05),and the observation group was lower than the control group(P＜0.05).The scores of GQOL-74 in the two groups were higher than those before operation(P＜0.05),and the observation group was higher than the control group(P＜0.05).The incidence of PHN in the observation group was lower than that in the control group(P＜0.05).There was no signifi-cant difference in the incidence of adverse reactions(P＞0.05).Conclusion:The dual needle bipolar pulsed radiofrequency therapy under ultrasound and CT dual guidance has a significant therapeutic effect on elderly patients with herpes zoster neuralgia,with no ad-verse reactions,and is worthy of promotion.

Abstract:Objective:To investigate the diagnostic efficacy of serum tumor markers for esophageal squamous cell carcinoma(ESCC)in northeast Sichuan province.Methods:The expression of miR-222-5p in esophageal tissues(observation group)and serum of 46 ESCC patients and 46 healthy subjects(control group)was detected by fluorescent quantitative PCR,and the relationship between the expression of serum miR-222-5p in ESCC and clinicopathological features was analyzed.Differential expression of miR-222-5p in tumor tissues and serum samples was analyzed by constructing receiver operating characteristic(ROC)curves to assess its diagnostic value for ESCC.Results:The serum level of miR-222-5p in the observation group was higher than that in the control group[(1.76±0.99)vs.(1.24±1.08),t=-2.396,P=0.019].The serum miR-222-5p level in ESCC stage Ⅰ was lower than that in ESCC stageⅡ and ESCC stage Ⅲ[(1.01±0.19)vs.(1.78±0.31)vs.(2.59±0.44),F=44.793,P＜0.001].The level of serum miR-222-5p in ESCC patients with distant metastasis was higher than that in ESCC patients without distant metastasis[(1.02±0.21)vs.(0.60±0.34),t=2.090,P=0.043].The serum miR-222-5p level of ESCC patients with lymph node metastasis was higher than that of ESCC patients without lymph node metastasis[(1.04±0.32)vs.(0.79±0.39),t=2.223,P=0.032].There was no significant difference in serum miR-222-5p levels between male and female ESCC patients[(1.01±1.33)vs.(1.07±1.61),t=-0.123,P=0.903].There was no significant difference in serum miR-222-5p levels between ≤ 65 years old and＞65 years old ESCC patients[(1.09±0.53)vs.(1.06±0.67),t=0.145,P=0.885].The serum miR-222-5p level in the pre-ESCC group was higher than that in the post-ESCC group[(2.46±1.65)vs.(1.85±0.99),t=2.141,P=0.035].ROC curve showed that when serum miR-222-5p was 0.393,the diagnostic efficacy of ESCC was the highest,the sensitivity was 58.70％,and the specificity was 80.60％.When miR-222-5p of tumor tissue was 0.435,the diagnostic efficacy of ESCC was the highest,and the sensitivity was 65.20％and the specificity was 78.30％.Conclusion:miR-222-5p has a certain predictive effect on the occurrence and development of ESCC in northeast Sichuan province,and shows moderate diagnostic efficacy.

Abstract:Objective:To explore the prognostic value of modified lymph node ratio(mLNR)and to construct a model to predict cancer-specific survival(CSS)in patients with non-muscle-invasive bladder cancer(NMIBC)after radical cystectomy(RC).Meth-ods:Eligible NMIBC patients were randomized in a 7∶3 ratio into a training group(n=651)and a validation group(n=280).Univa-riate and multivariate Cox regression analyses were used to screen for independent predictors by stepwise regression and to check for multicollinearity between variables,and then nomograms predicting CSS were created and validated.The developed risk classification system was tested by Kaplan-Meyer survival analysis and log-rank test.Results:Age,marital status,N-staging,radiotherapy and mLNR were independent prognostic factors for CSS in patients with NMBIC.The nomogram C index for the training cohort was 0.706(95％CI:0.661～0.751).In the training cohort,the AUCs for predicting 1,5,and 10 years CSS were 0.799,0.736,and 0.732,respectively.In the validation cohort,the AUCs for predicting 1,5,and 10 year CSS were 0.543,0.703,and 0.668,respectively.Conclusion:The mLNR is a new independent prognostic indicator,and the prognostic model developed based on it predicted 1,5,and 10 year CSS slight-ly better than the AJCC staging system,which is conducive to the enrichment of prognostic indicators for cancer.

Abstract:< positive > 1 case data patient, female, 61 years old. He was admitted to the hospital on March 10, 2023 because of "diarrhea for 3 years and aggravated for half a year". The patient had no obvious cause of diarrhea 3 years ago, presenting yellow watery stools, no mucus, pus and blood, defecating about 10 times / D, no abdominal pain, abdominal distension, nausea and vomiting, no acid regurgitation and heartburn, and the symptoms were relieved after oral administration of "omeprazole, levofloxacin, probiotics, montmorillonite powder", but the diarrhea was repeated after discontinuation. Half a year ago, the diarrhea of the patient was more serious than before, the number of defecation increased to 10-20 times / D, accompanied by acid reflux and heartburn, and the body weight decreased by about 10 kg in recent half a year.

Abstract:Objective:To explore the application effect of Plato theory-based risk control management in the in-hospital safe transport of elderly patients in post-anesthesia care unit(PACU).Methods:160 elderly patients who underwent PACU resuscitation and were transported in the backyard were selected as the research subjects.According to different transportation management methods,the patients were divided into a conventional group and a Pareto group,with 80 patients in each group.The routine group was treated with implement routine transportation management,and the Plato group was treated with risk control transfer management based on Plato's theory.The pre-transport indicators[Bispectral index(BIS)value,modified Aldrete score,saturation of arterial oxygen(SpO2),partial pressure of end-tidal carbon dioxide(PET CO2),mean arterial pressure(MAP)],priority risk values during anesthesia recovery,transport conditions(transport preparation time,transport duration and transfer delay rate),adverse events during transport and patients'satisfaction with management measures were compared between the two groups.Results:The BIS,modified Aldrete score and SpO2 in the Plato group at the time of transferring from PACU were higher than those in the conventional group while the PETCO2 and MAP were lower than those in the conventional group(P＜0.05).The risk values scores,transport preparation time,transport time and transfer delay rate were lower than those in the conventional group,and patients'satisfaction with management was higher than that in the conventional group(P＜0.05).Conclusion:Plato theory-based risk control management can reduce the risk values and transport time of intra-hospital transport of elderly patients in PACU,and it is helpful to reduce the adverse events during transport and strengthen the safety of transport,and it has high patient satisfaction.

Abstract:Objective:To explore the influence of holistic nursing based on evidence-based concept on middle-aged and elderly patients with pelvic floor dysfunction(PFD).Methods:Clinical data of 100 middle-aged and elderly PFD patients were retrospectively analyzed.According to different nursing methods,the above patients were classified into control group(n=47,routine nursing)and ob-servation group(n=53,routine nursing+holistic nursing based on evidence-based concept).The negative emotions scores,pelvic floor muscle strength grading,pelvic floor function and quality of life before and after nursing and nursing satisfaction were compared be-tween the two groups.Results:After nursing,the anxiety scores,depression scores,and the scores of pelvic floor function in the observa-tion group were lower than those in the control group(P＜0.05).Compared with the control group,the pelvic muscle strength grade of the observation group was better,and the life quality score of the observation group was higher(P＜0.05).The total satisfaction of nursing in observation group(93.34％)was higher than that in control group(78.72％)(P＜0.05).Conclusion:Holistic nursing based on evidence-based concept can improve the negative emotions,pelvic floor muscle strength and pelvic floor function of PFD pa-tients,and it has a good rehabilitation effect and can also enhance the quality of life and nursing satisfaction of patients.

Abstract:Objective:To investigate the application effect of positive suggestion in the nursing of patients with acute exacerba-tion of chronic obstructive pulmonary disease(AECOPD).Methods:80 patients with AECOPD were divided into intervention group(n=40)and control group(n=40)according to different nursing methods.The control group was given routine nursing,and the inter-vention group was combined with positive suggestion intervention on the basis of the control group.The intervention time of both groups was 1 week.Respiratory function,anxiety/depression/fear state,sleep quality and quality of life were compared between the two groups before intervention and 1 week after intervention,and patients'nursing satisfaction was investigated before discharge.Results:After 1 week of intervention,forced vital capacity(FVC),forced expiratory volume at the first second(FEV1)and FEV1/FVC in both groups were higher than before intervention(P＜0.05),and those in intervention group were higher than those in control group(P＜0.05).The scores of self-rating anxiety scale(SAS),self-rating depression scale(SDS)and visual analogue scale(VAS)in both groups were lower than before intervention(P＜0.05),and the scores in intervention group were lower(P＜0.05).The scores of symptoms,mobil-ity and life impact of disease on Pittsburgh sleep quality index(PSQI)and Saint George's respiratory questionnaire(SGRQ)of both groups were lower than before intervention(P＜0.05),and intervention group was lower than the control group(P＜0.05).Before discharge,the nursing satisfaction of patients in the intervention group was higher than that in the control group(95.00％vs.80.00％,P＜0.05).Conclusion:The positive suggestion effect can effectively improve the respiratory function of AECOPD patients,relieve their anxiety,depression and fear,and improve their sleep quality and quality of life.